Vouchering DNA-barcoded specimens: test of a nondestructive extraction protocol for terrestrial arthropods

Vouchering DNA-barcoded specimens: test of a nondestructive extraction protocol for terrestrial arthropods

Morphology-based keys support accurate identification of many taxa. However, identification can be difficult for taxa that are either not well studied, very small, members of cryptic species complexes, or represented by immature stages. For such cases, DNA barcodes may provide diagnostic characters....

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Bibliographic Details
Published in:Molecular ecology notes Vol. 7; no. 6; pp. 915 - 924
Main Authors: Rowley, D.L, Coddington, J.A, Gates, M.W, Norrbom, A.L, Ochoa, R.A, Vandenberg, N.J, Greenstone, M.H
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-11-2007
Blackwell Publishing Ltd
Subjects:
Acarina
animal morphology
animal taxonomy
Arachnida
Araneae
Arthropoda
arthropods
barcode
COI gene
Coleoptera
cytochrome oxidase I
cytochrome-c oxidase
Diptera
DNA barcodes
DNA barcoding
DNA extraction
extraction
Hymenoptera
Insecta
mitochondrial DNA
molecular systematics
nondestructive methods
taxonomy
type collections
voucher
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Summary:Morphology-based keys support accurate identification of many taxa. However, identification can be difficult for taxa that are either not well studied, very small, members of cryptic species complexes, or represented by immature stages. For such cases, DNA barcodes may provide diagnostic characters. Ecologists and evolutionary biologists deposit museum vouchers to document the species studied in their research. If DNA barcodes are to be used for identification, then both the DNA and the specimen from which it was extracted should be vouchered. We describe a protocol for the nondestructive extraction of DNA from terrestrial arthropods, using as examples members of the orders Acarina, Araneae, Coleoptera, Diptera, and Hymenoptera chosen to represent the ranges in size, overall sclerotization, and delicacy of key morphological characters in the group. We successfully extracted sequenceable DNA from all species after 1-4 h of immersion in extraction buffer. The extracted carcasses, processed and imaged using protocols standard for the taxon, were distinguishable from closely related species, and adequate as morphological vouchers. We provide links from the carcasses and DNA vouchers to image (MorphBank) and sequence (GenBank) databases.
Bibliography:http://hdl.handle.net/10113/14100
http://dx.doi.org/10.1111/j.1471-8286.2007.01905.x
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ArticleID:MEN1905
These authors contributed equally to this research.
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ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2007.01905.x