Search Results - "VanDusen, J"

A reference map of murine cardiac transcription factor chromatin occupancy identifies dynamic and conserved enhancers by Akerberg, Brynn N., Gu, Fei, VanDusen, Nathan J., Zhang, Xiaoran, Dong, Rui, Li, Kai, Zhang, Bing, Zhou, Bin, Sethi, Isha, Ma, Qing, Wasson, Lauren, Wen, Tong, Liu, Jinhua, Dong, Kunzhe, Conlon, Frank L., Zhou, Jiliang, Yuan, Guo-Cheng, Zhou, Pingzhu, Pu, William T.

“…Mapping the chromatin occupancy of transcription factors (TFs) is a key step in deciphering developmental transcriptional programs. Here we use biotinylated…”
Get full text

Analysis of Cardiac Myocyte Maturation Using CASAAV, a Platform for Rapid Dissection of Cardiac Myocyte Gene Function In Vivo by Guo, Yuxuan, VanDusen, Nathan J, Zhang, Lina, Gu, Weiliang, Sethi, Isha, Guatimosim, Silvia, Ma, Qing, Jardin, Blake D, Ai, Yulan, Zhang, Donghui, Chen, Biyi, Guo, Ang, Yuan, Guo-Cheng, Song, Long-Sheng, Pu, William T

“…RATIONALE:Loss-of-function studies in cardiac myocytes (CMs) are currently limited by the need for appropriate conditional knockout alleles. The factors that…”
Get full text

Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation by VanDusen, Nathan J., Lee, Julianna Y., Gu, Weiliang, Butler, Catalina E., Sethi, Isha, Zheng, Yanjiang, King, Justin S., Zhou, Pingzhu, Suo, Shengbao, Guo, Yuxuan, Ma, Qing, Yuan, Guo-Cheng, Pu, William T.

“…The forward genetic screen is a powerful, unbiased method to gain insights into biological processes, yet this approach has infrequently been used in vivo in…”
Get full text

CRISPR-Cas9-mediated homology-directed repair for precise gene editing by Liao, Hongyu, Wu, Jiahao, VanDusen, Nathan J., Li, Yifei, Zheng, Yanjiang

“…CRISPR-Cas9-mediated homology-directed repair (HDR) is a versatile platform for creating precise site-specific DNA insertions, deletions, and substitutions…”
Get full text

Massively Parallel Reporter Assays for High-Throughput In Vivo Analysis of Cis-Regulatory Elements by Zheng, Yanjiang, VanDusen, Nathan J

“…The rapid improvement of descriptive genomic technologies has fueled a dramatic increase in hypothesized connections between cardiovascular gene expression and…”
Get full text

Hierarchical and stage-specific regulation of murine cardiomyocyte maturation by serum response factor by Guo, Yuxuan, Jardin, Blake D., Zhou, Pingzhu, Sethi, Isha, Akerberg, Brynn N., Toepfer, Christopher N, Ai, Yulan, Li, Yifei, Ma, Qing, Guatimosim, Silvia, Hu, Yongwu, Varuzhanyan, Grigor, VanDusen, Nathan J., Zhang, Donghui, Chan, David C., Yuan, Guo-Cheng, Seidman, Christine E., Seidman, Jonathan G., Pu, William T.

“…After birth, cardiomyocytes (CM) acquire numerous adaptations in order to efficiently pump blood throughout an animal’s lifespan. How this maturation process…”
Get full text

Hand2 Is an Essential Regulator for Two Notch-Dependent Functions within the Embryonic Endocardium by VanDusen, Nathan J., Casanovas, Jose, Vincentz, Joshua W., Firulli, Beth A., Osterwalder, Marco, Lopez-Rios, Javier, Zeller, Rolf, Zhou, Bin, Grego-Bessa, Joaquim, De La Pompa, José Luis, Shou, Weinian, Firulli, Anthony B.

“…The basic-helix-loop-helix (bHLH) transcription factor Hand2 plays critical roles during cardiac morphogenesis via expression and function within myocardial,…”
Get full text

Author Correction: Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation by VanDusen, Nathan J., Lee, Julianna Y., Gu, Weiliang, Butler, Catalina E., Sethi, Isha, Zheng, Yanjiang, King, Justin S., Zhou, Pingzhu, Suo, Shengbao, Guo, Yuxuan, Ma, Qing, Yuan, Guo-Cheng, Pu, William T.

Get full text

Precise genome-editing in human diseases: mechanisms, strategies and applications by Zheng, Yanjiang, Li, Yifei, Zhou, Kaiyu, Li, Tiange, VanDusen, Nathan J., Hua, Yimin

“…Precise genome-editing platforms are versatile tools for generating specific, site-directed DNA insertions, deletions, and substitutions. The continuous…”
Get full text

Fkbp1a controls ventricular myocardium trabeculation and compaction by regulating endocardial Notch1 activity by Chen, Hanying, Zhang, Wenjun, Sun, Xiaoxin, Yoshimoto, Momoko, Chen, Zhuang, Zhu, Wuqiang, Liu, Jijia, Shen, Yadan, Yong, Weidong, Li, Deqiang, Zhang, Jin, Lin, Yang, Li, Baiyan, VanDusen, Nathan J, Snider, Paige, Schwartz, Robert J, Conway, Simon J, Field, Loren J, Yoder, Mervin C, Firulli, Anthony B, Carlesso, Nadia, Towbin, Jeffrey A, Shou, Weinian

“…Trabeculation and compaction of the embryonic myocardium are morphogenetic events crucial for the formation and function of the ventricular walls. Fkbp1a…”
Get full text

Loss of Hand2 in a population of Periostin lineage cells results in pronounced bradycardia and neonatal death by VanDusen, Nathan J., Vincentz, Joshua W., Firulli, Beth A., Howard, Marthe J., Rubart, Michael, Firulli, Anthony B.

“…The Periostin Cre (Postn-Cre) lineage includes endocardial and neural crest derived mesenchymal cells of the cardiac cushions, neural crest-derived components…”
Get full text

Cardiac Regeneration: Lessons From Development by Galdos, Francisco X, Guo, Yuxuan, Paige, Sharon L, VanDusen, Nathan J, Wu, Sean M, Pu, William T

“…Palliative surgery for congenital heart disease has allowed patients with previously lethal heart malformations to survive and, in most cases, to thrive…”
Get full text

Cardiac Regeneration by Galdos, Francisco X, Guo Yuxuan, Paige, Sharon L, VanDusen, Nathan J, Wu, Sean M, Pu, William T

“…Palliative surgery for congenital heart disease has allowed patients with previously lethal heart malformations to survive and, in most cases, to thrive…”
Get full text

Efficient In Vivo Homology-Directed Repair Within Cardiomyocytes by Zheng, Yanjiang, VanDusen, Nathan J., Butler, Catalina E., Ma, Qing, King, Justin S., Pu, William T.

Get full text

In Vivo Dissection of Chamber-Selective Enhancers Reveals Estrogen-Related Receptor as a Regulator of Ventricular Cardiomyocyte Identity by Cao, Yangpo, Zhang, Xiaoran, Akerberg, Brynn N., Yuan, Haiyun, Sakamoto, Tomoya, Xiao, Feng, VanDusen, Nathan J., Zhou, Pingzhu, Sweat, Mason E., Wang, Yi, Prondzynski, Maksymilian, Chen, Jian, Zhang, Yan, Wang, Peizhe, Kelly, Daniel P., Pu, William T.

“…Cardiac chamber-selective transcriptional programs underpin the structural and functional differences between atrial and ventricular cardiomyocytes (aCMs and…”
Get full text

Dynamic changes in P300 enhancers and enhancer-promoter contacts control mouse cardiomyocyte maturation by Zhou, Pingzhu, VanDusen, Nathan J., Zhang, Yanchun, Cao, Yangpo, Sethi, Isha, Hu, Rong, Zhang, Shuo, Wang, Guangyu, Ye, Lincai, Mazumdar, Neil, Chen, Jian, Zhang, Xiaoran, Guo, Yuxuan, Li, Bin, Ma, Qing, Lee, Julianna Y., Gu, Weiliang, Yuan, Guo-Cheng, Ren, Bing, Chen, Kaifu, Pu, William T.

“…Cardiomyocyte differentiation continues throughout murine gestation and into the postnatal period, driven by temporally regulated expression changes in the…”
Get full text

Inhibition of Calcium Oxalate Crystal Growth in vitro by Uropontin: Another Member of the Aspartic Acid-Rich Protein Superfamily by Shiraga, H., Min, W., VanDusen, W. J., Clayman, M. D., Miner, D., Terrell, C. H., Sherbotie, J. R., Foreman, J. W., Przysiecki, C., Neilson, E. G., Hoyer, J. R.

“…The majority of human urinary stones are primarily composed of calcium salts. Although normal urine is frequently supersaturated with respect to calcium…”
Get full text

CASAAV: A CRISPR-Based Platform for Rapid Dissection of Gene Function In Vivo by VanDusen, Nathan J, Guo, Yuxuan, Gu, Weiliang, Pu, William T

“…In vivo loss-of-function studies are currently limited by the need for appropriate conditional knockout alleles. CRISPR/Cas9 is a powerful tool commonly used…”
Get more information

Green fluorescent protein in Saccharomyces cerevisiae: Real-time studies of the GAL1 promoter by Li, Jincai, Wang, Shu, VanDusen, William J., Schultz, Loren D., George, Hugh A., Herber, Wayne K., Chae, Hee Jeong, Bentley, William E., Rao, Govind

“…Green fluorescent protein (GFP) was used to study the regulation of the galactose‐inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was…”
Get full text

Hand2 Loss-of-Function in Hand1-Expressing Cells Reveals Distinct Roles in Epicardial and Coronary Vessel Development by Barnes, Ralston M, Firulli, Beth A, VanDusen, Nathan J, Morikawa, Yuka, Conway, Simon J, Cserjesi, Peter, Vincentz, Joshua W, Firulli, Anthony B

“…RATIONALE:The basic helix–loop–helix (bHLH) transcription factors Hand1 and Hand2 are essential for embryonic development. Given their requirement for…”
Get full text