Search Results - "Van den Heuvel, Joop"

Baculovirus-free insect cell expression system for high yield antibody and antigen production by Korn, Janin, Schäckermann, Dorina, Kirmann, Toni, Bertoglio, Federico, Steinke, Stephan, Heisig, Janyn, Ruschig, Maximilian, Rojas, Gertrudis, Langreder, Nora, Wenzel, Esther Veronika, Roth, Kristian Daniel Ralph, Becker, Marlies, Meier, Doris, van den Heuvel, Joop, Hust, Michael, Dübel, Stefan, Schubert, Maren

“…Antibodies are essential tools for therapy and diagnostics. Yet, production remains expensive as it is mostly done in mammalian expression systems. As most…”
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A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells by Baser, Bahar, Spehr, Johannes, Büssow, Konrad, van den Heuvel, Joop

“…•Presentation of a protocol to establish a binary RMCE expression system.•Generation of two binary RMCE stable CHO Lec3.2.8.1 cell pools.•Cumulative and…”
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Cryo-EM structure of Hepatitis C virus IRES bound to the human ribosome at 3.9-Å resolution by Quade, Nick, Boehringer, Daniel, Leibundgut, Marc, van den Heuvel, Joop, Ban, Nenad

“…Hepatitis C virus (HCV), a widespread human pathogen, is dependent on a highly structured 5′-untranslated region of its mRNA, referred to as internal ribosome…”
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Enzyme‐Activated, Chemiluminescent Siderophore‐Dioxetane Probes Enable the Selective and Highly Sensitive Detection of Bacterial Pathogens by Peukert, Carsten, Popat Gholap, Sachin, Green, Ori, Pinkert, Lukas, Heuvel, Joop, Ham, Marco, Shabat, Doron, Brönstrup, Mark

“…The sensitive detection of bacterial infections is a prerequisite for their successful treatment. The use of a chemiluminescent readout was so far hampered by…”
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Meteorin-like promotes heart repair through endothelial KIT receptor tyrosine kinase by Reboll, Marc R, Klede, Stefanie, Taft, Manuel H, Cai, Chen-Leng, Field, Loren J, Lavine, Kory J, Koenig, Andrew L, Fleischauer, Jenni, Meyer, Johann, Schambach, Axel, Niessen, Hans W, Kosanke, Maike, van den Heuvel, Joop, Pich, Andreas, Bauersachs, Johann, Wu, Xuekun, Zheng, Linqun, Wang, Yong, Korf-Klingebiel, Mortimer, Polten, Felix, Wollert, Kai C

“…Effective tissue repair after myocardial infarction entails a vigorous angiogenic response, guided by incompletely defined immune cell-endothelial cell…”
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Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate by Kleinboelting, Silke, Diaz, Ana, Moniot, Sebastien, van den Heuvel, Joop, Weyand, Michael, Levin, Lonny R., Buck, Jochen, Steegborn, Clemens

“…cAMP is an evolutionary conserved, prototypic second messenger regulating numerous cellular functions. In mammals, cAMP is synthesized by one of 10 homologous…”
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Design, Synthesis, and Pharmacological Evaluation of Second-Generation Soluble Adenylyl Cyclase (sAC, ADCY10) Inhibitors with Slow Dissociation Rates by Miller, Michael, Rossetti, Thomas, Ferreira, Jacob, Ghanem, Lubna, Balbach, Melanie, Kaur, Navpreet, Levin, Lonny R., Buck, Jochen, Kehr, Maria, Coquille, Sandrine, van den Heuvel, Joop, Steegborn, Clemens, Fushimi, Makoto, Finkin-Groner, Efrat, Myers, Robert W., Kargman, Stacia, Liverton, Nigel J., Huggins, David J., Meinke, Peter T.

“…Soluble adenylyl cyclase (sAC: ADCY10) is an enzyme involved in intracellular signaling. Inhibition of sAC has potential therapeutic utility in a number of…”
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Identifying parameters to improve the reproducibility of transient gene expression in High Five cells by Bleckmann, Maren, Schürig, Margitta, Endres, Michelle, Samuels, Anke, Gebauer, Daniela, Konisch, Nadine, van den Heuvel, Joop

“…Virus-free, transient gene expression (TGE) in High Five cells was recently presented as an efficient protein production method. However, published TGE…”
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Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli by DRESLER, Karolin, VAN DEN HEUVEL, Joop, MÜLLER, Rolf-Joachim, DECKWER, Wolf-Dieter

“…The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6)…”
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Discovery of LRE1 as a specific and allosteric inhibitor of soluble adenylyl cyclase by Ramos-Espiritu, Lavoisier, Kleinboelting, Silke, Navarrete, Felipe A, Alvau, Antonio, Visconti, Pablo E, Valsecchi, Federica, Starkov, Anatoly, Manfredi, Giovanni, Buck, Hannes, Adura, Carolina, Zippin, Jonathan H, van den Heuvel, Joop, Glickman, J Fraser, Steegborn, Clemens, Levin, Lonny R, Buck, Jochen

“…Editorial summary A high-throughput screen using a mass-spectrometry-based assay results in the identification of LRE1 as an inhibitor of HCO 3 − /Ca 2+…”
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Synthetic studies of cystobactamids as antibiotics and bacterial imaging carriers lead to compounds with high in vivo efficacy by Testolin, Giambattista, Cirnski, Katarina, Rox, Katharina, Prochnow, Hans, Fetz, Verena, Grandclaudon, Charlotte, Mollner, Tim, Baiyoumy, Alain, Ritter, Antje, Leitner, Christian, Krull, Jana, van den Heuvel, Joop, Vassort, Aurelie, Sordello, Sylvie, Hamed, Mostafa M, Elgaher, Walid A M, Herrmann, Jennifer, Hartmann, Rolf W, Müller, Rolf, Brönstrup, Mark

“…There is an alarming scarcity of novel chemical matter with bioactivity against multidrug-resistant Gram-negative bacterial pathogens. Cystobactamids, recently…”
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Screening of Membrane Protein Production by Comparison of Transient Expression in Insect and Mammalian Cells by Kaipa, Jagan Mohan, Krasnoselska, Ganna, Owens, Raymond J, van den Heuvel, Joop

“…Membrane proteins are difficult biomolecules to express and purify. In this paper, we compare the small-scale production of six selected eukaryotic integral…”
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Systematical assessment of the impact of single spike mutations of SARS-CoV-2 Omicron sub-variants on the neutralization capacity of post-vaccination sera by Katzmarzyk, Maeva, Clesle, Denise Christine, van den Heuvel, Joop, Hoffmann, Markus, Garritsen, Henk, Pöhlmann, Stefan, Jacobsen, Henning, Čičin-Šain, Luka

“…The evolution of novel SARS-CoV-2 variants significantly affects vaccine effectiveness. While these effects can only be studied retrospectively, neutralizing…”
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ER intrabody-mediated inhibition of interferon α secretion by mouse macrophages and dendritic cells by Büssow, Konrad, Themann, Philipp, Luu, Sabine, Pentrowski, Paul, Harting, Claudia, Majewski, Mira, Vollmer, Veith, Köster, Mario, Grashoff, Martina, Zawatzky, Rainer, Van den Heuvel, Joop, Kröger, Andrea, Böldicke, Thomas

“…Interferon α (IFNα) counteracts viral infections by activating various IFNα-stimulated genes (ISGs). These genes encode proteins that block viral transport…”
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Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells by Bleckmann, Maren, Schürig, Margitta, Chen, Fang-Fang, Yen, Zen-Zen, Lindemann, Nils, Meyer, Steffen, Spehr, Johannes, van den Heuvel, Joop

“…The Baculovirus Expression Vector System (BEVS) is widely used to produce high amounts of recombinant proteins. Nevertheless, generating recombinant…”
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Extending the Host Range of Listeria monocytogenes by Rational Protein Design by Wollert, Thomas, Pasche, Bastian, Rochon, Maike, Deppenmeier, Stefanie, van den Heuvel, Joop, Gruber, Achim D., Heinz, Dirk W., Lengeling, Andreas, Schubert, Wolf-Dieter

“…In causing disease, pathogens outmaneuver host defenses through a dedicated arsenal of virulence determinants that specifically bind or modify individual host…”
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Genomic Analysis and Isolation of RNA Polymerase II Dependent Promoters from Spodoptera frugiperda by Bleckmann, Maren, Fritz, Markus H-Y, Bhuju, Sabin, Jarek, Michael, Schürig, Margitta, Geffers, Robert, Benes, Vladimir, Besir, Hüseyin, van den Heuvel, Joop

“…The Baculoviral Expression Vector System (BEVS) is the most commonly used method for high expression of recombinant protein in insect cells. Nevertheless,…”
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Investigations on the mode of action of gephyronic acid, an inhibitor of eukaryotic protein translation from myxobacteria by Muthukumar, Yazh, Münkemer, Johanna, Mathieu, Daniel, Richter, Christian, Schwalbe, Harald, Steinmetz, Heinrich, Kessler, Wolfgang, Reichelt, Joachim, Beutling, Ulrike, Frank, Ronald, Büssow, Konrad, van den Heuvel, Joop, Brönstrup, Mark, Taylor, Richard E, Laschat, Sabine, Sasse, Florenz

“…The identification of inhibitors of eukaryotic protein biosynthesis, which are targeting single translation factors, is highly demanded. Here we report on a…”
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Baculovirus-Free SARS-CoV-2 Virus-like Particle Production in Insect Cells for Rapid Neutralization Assessment by Jaron, Marcel, Lehky, Michael, Zarà, Marta, Zaydowicz, Chris Nicole, Lak, Aidin, Ballmann, Rico, Heine, Philip Alexander, Wenzel, Esther Veronika, Schneider, Kai-Thomas, Bertoglio, Federico, Kempter, Susanne, Köster, Reinhard Wolfgang, Barbieri, Silvia Stella, van den Heuvel, Joop, Hust, Michael, Dübel, Stefan, Schubert, Maren

“…Virus-like particles (VLPs) resemble authentic virus while not containing any genomic information. Here, we present a fast and powerful method for the…”
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Multi-host expression system for recombinant production of challenging proteins by Meyer, Steffen, Lorenz, Carmen, Baser, Bahar, Wördehoff, Mona, Jäger, Volker, van den Heuvel, Joop

“…Recombinant production of complex eukaryotic proteins for structural analyses typically requires a profound screening process to identify suitable constructs…”
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