Oral administration of deuterium-labelled polyamines to sucking rat pups: luminal uptake, metabolic fate and effects on gastrointestinal maturation

Non-physiological amounts of oral polyamines have been reported to induce precocious gut maturation in rat pups. The aim of the present study was to investigate organ distribution and metabolic fate of orally administered stable-isotopically labelled polyamines in rat pups. Pups received tetradeuter...

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Bibliographic Details
Published in:	British journal of nutrition Vol. 78; no. 4; pp. 639 - 654
Main Authors:	Dorhout, Bernard, Van Faassen, Anet, Van Beusekom, Christien M., Kingma, Anneke W., De Hoog, Elly, Nagel, Gijs T., Karrenbeld, Arend, Boersma, E. Rudy, Muskiet, Frits A. J.
Format:	Journal Article
Language:	English
Published:	Cambridge, UK Cambridge University Press 01-10-1997
Subjects:	Amine Oxidase (Copper-Containing) - metabolism Animals Animals, Suckling - anatomy & histology Animals, Suckling - growth & development Animals, Suckling - metabolism Biological and medical sciences Cecum - metabolism Colon - metabolism Deuterium Disaccharidases - metabolism Erythrocytes - metabolism Fundamental and applied biological sciences. Psychology General Nutrition Gut maturation Heart - anatomy & histology Ileum - anatomy & histology Ileum - growth & development Ileum - metabolism Intestine. Mesentery Kidney - anatomy & histology Liver - anatomy & histology Metabolism Organ Size Polyamines Polyamines - administration & dosage Polyamines - metabolism Putrescine - administration & dosage Putrescine - metabolism Rats Rats, Wistar Spermidine - administration & dosage Spermidine - metabolism Spermine - administration & dosage Spermine - metabolism Vertebrates: digestive system Polyamines Metabolism Gut maturation Polyamine Rat Digestive system Rodentia Gut Oral administration Suckling animal Ripening Vertebrata Mammalia Polycation Newborn animal Body compartment Supplementation
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Summary:	Non-physiological amounts of oral polyamines have been reported to induce precocious gut maturation in rat pups. The aim of the present study was to investigate organ distribution and metabolic fate of orally administered stable-isotopically labelled polyamines in rat pups. Pups received tetradeuterium-labelled putrescine (Pu-d4; 3 μmol), spermidine (Sd-d4; 5 μmol), spermine (Sp-d4; 3 μmol), or physiological saline twice daily on postnatal days 7–10 or 12–15. They were killed on days 10 and 15. We determined activities of ileal lactase (EC 3.2.1.23), maltase (EC 3.2.1.20), sucrase (EC 3.2.1.48) and diamine oxidase (EC 1.4.3.6) and established villus and crypt lengths. Polyamines and their labelling percentages in organs were determined by GC and mass fragmentography. Treatments did not affect growth rate, but caused lower weights of liver, kidneys and heart. Maltase activity increased, lactase decreased, whereas sucrase and diamine oxidase did not change. Villus and crypt lengths increased. Organ polyamine pools were labelled to different extents. Irrespective of the orally administered polyamine, all organs contained Pu-d4, Sd-d4 and Sp-d4. Administered Pu-d4 and Sd-d4 were recovered mainly as Sd-d4, whereas Sp-d4 was recovered as Sp-d4 and Sd-d4. Total polyamines in a caecum, colon and erythrocytes increased, but increases were only to a minor extent with regard to labelled polyamines. Our data confirm precocious gut maturation by exogenous polyamines. Putrescine appears to be the limiting factor. The exogenous polyamines were distributed among all investigated organs. They are not only used for the synthesis of higher polyamines, but also retroconverted to their precursors. Changes in erythrocyte polyamine contents suggest precocious stimulation of erythropoiesis.
Bibliography:	PII:S0007114597001670 ark:/67375/6GQ-1XCCLC12-M ArticleID:00167 istex:2F68D6AEB2A09FA9A09C3B5162CBBC22CF97BE99 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0007-1145 1475-2662
DOI:	10.1079/BJN19970180