Search Results - "Van Blokland, Rik"

single transgene locus triggers both transcriptional and post-transcriptional silencing through double-stranded RNA production by Mourrain, Philippe, Blokland, Rik van, Kooter, Jan M, Vaucheret, Hervé

“…Silencing of a target locus by an unlinked silencing locus can result from transcription inhibition (transcriptional gene silencing; TGS) or mRNA degradation…”
Get full text

Various Expression-Augmenting DNA Elements Benefit from STAR-Select, a Novel High Stringency Selection System for Protein Expression by Otte, Arie P., Kwaks, Ted H. J., Van Blokland, Rik J. M., Sewalt, Richard G. A. B., Verhees, John, Klaren, Vincent N. A., Siersma, Tjalling K., Korse, Hans W. M., Teunissen, Nannette C., Botschuijver, Sara, Van Mer, Charl, Man, Sue Y.

“…The creation of highly productive mammalian cell lines often requires the screening of large numbers of clones, and even then expression levels are often low…”
Get full text

Transcriptional and posttranscriptional gene silencing are mechanistically related by Sijen, Titia, Vijn, Irma, Rebocho, Alexandra, van Blokland, Rik, Roelofs, Dick, Mol, Joseph N.M., Kooter, Jan M.

“…Two distinct gene-silencing phenomena are observed in plants: transcriptional gene silencing (TGS), which involves decreased RNA synthesis because of promoter…”
Get full text

Identification of anti-repressor elements that confer high and stable protein production in mammalian cells by Otte, Arie P, Kwaks, Ted H.J, Barnett, Phil, Hemrika, Wieger, Siersma, Tjalling, Sewalt, Richard G.A.B, Satijn, David P.E, Brons, Janynke F, van Blokland, Rik, Kwakman, Paul, Kruckeberg, Arle L, Kelder, Angèle

“…The expression of transgenic proteins is often low and unstable over time, a problem that may be due to integration of the transgene in repressed chromatin. We…”
Get full text

Post‐transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats by Stam, Maike, Bruin, Rob, Kenter, Susan, Hoorn, Renier A.L., Blokland, Rik, Mol, Joseph N.M., Kooter, Jan M.

“…Summary To induce post‐transcriptional silencing of flower pigmentation genes by homologous sense transgenes in transgenic petunias, it is not necessary for…”
Get full text

Various expression-augmenting DNA elements benefit from STAR-select, a novel high stringency selection system for protein expression by OTTE, Arie P, KWAKS, Ted H. J, VAN MER, Charl, MAN, Sue Y, VAN BLOKLAND, Rik J. M, SEWALT, Richard G. A. B, VERHEES, John, KLAREN, Vincent N. A, SIERSMA, Tjalling K, KORSE, Hans W. M, TEUNISSEN, Nannette C, BOTSCHUIJVER, Sara

Get full text

The Use of a Stringent Selection System Allows the Identification of DNA Elements that Augment Gene Expression by Hoeksema, Femke, van Blokland, Rik, Siep, Michel, Hamer, Karien, Siersma, Tjalling, den Blaauwen, Jan, Verhees, John, Otte, Arie P.

“…The use of high stringency selection systems often results in the induction of very few recombinant mammalian cell lines, which limits the ability to isolate a…”
Get full text

Distinct features of post-transcriptional gene silencing by antisense transgenes in single copy and inverted T-DNA repeat loci by Stam, M, Bruin, R. de, Blokland, R. van, Hoorn, R.A.L. van der, Mol, J.N.M, Kooter, J.M

“…Summary The application of antisense transgenes in plants is a powerful tool to inhibit gene expression. The underlying mechanism of this inhibition is still…”
Get full text

Erratum: Identification of anti-repressor elements that confer high and stable protein production in mammalian cells by Kwaks, Ted HJ, Barnett, Phil, Hemrika, Wieger, Siersma, Tjalling, Sewalt, Richard GAB, Satijn, David PE, Brons, Janynke F, van Blokland, Rik, Kwakman, Paul, Kruckeberg, Arle, Kelder, Angèle, Otte, Arie P

Get full text

Corrigenda: Identification of anti-repressor elements that confer high and stable protein production in mammalian cells by Ted HJ Kwaks, Barnett, Phil, Hemrika, Wieger, Siersma, Tjalling, Richard GAB Sewalt, David PE Satijn, Brons, Janynke F, Rik van Blokland, Kwakman, Paul, Kruckeberg, Arle, Ang? Kelder, Otte, Arie P

Get full text

Developmental abnormalities associated with deoxyadenosine methylation in transgenic tobacco by Van Blokland, Rik, Ross, Simon, Corrado, Giandomenico, Scollan, Claire, Meyer, Peter

“…Summary As in other higher eukaryotes, DNA methylation in plants is predominantly found at deoxycytosine residues, while deoxyadenosine residues are not…”
Get full text

Identification of anti-repressor elements that confer high and stable protein production in mammalian cells by Kwaks, Ted HJ, Barnett, Phil, Hemrika, Wieger, Siersma, Tjalling, Sewalt, Richard GAB, Satijn, David PE, Brons, Janynke F, van Blokland, Rik, Kwakman, Paul, Kruckeberg, Arle, Kelder, Angèle, Otte, Arie P

Get full text

Chalcone Synthase Promoters in Petunia Are Active in Pigmented and Unpigmented Cell Types by Koes, Ronald E., van Blokland, Rik, Quattrocchio, Francesca, van Tunen, Arjen J., Mol, Joseph N. M.

Get full text

Gibberellic acid regulates chalcone synthase gene transcription in the corolla of Petunia hybrida by Weiss, D. (Vrije Universiteit, Amsterdam, Netherlands), Blokland, R. van, Kooter, J.M, Mol, J.N.M, Tunen, A.J. van

“…The pigmentation of Petunia hybrida corollas is regulated by gibberellic acid (GA3). It controls the increase of flavonoid enzyme levels and their…”
Get full text

Gibberellic Acid Regulates Chalcone Synthase Gene Transcription in the Corolla of Petunia hybrida 1 by Weiss, David, van Blokland, Rik, Kooter, Jan M., Mol, Joseph N. M., van Tunen, Arjen J.

“…The pigmentation of Petunia hybrida corollas is regulated by gibberellic acid (GA 3 ). It controls the increase of flavonoid enzyme levels and their…”
Get full text

Chalcone synthase promoters in petunia are active in pigmented and unpigmented cell types by Koes, Ronald E., van Blokland, Rik, Quattrocchio, Francesca, van Tunen, Arjen J., Joseph N. M. Mol

“…Chalcone synthase (CHS) catalyzes the first step in the biosynthesis of flavonoids that function in flower pigmentation, protection against stress, and…”
Get full text