Parainfluenza Virus Type 1 Induces Epithelial IL-8 Production via p38-MAPK Signalling

Human parainfluenza virus type 1 (HPIV-1) is the most common cause of croup in infants. The aim of this study was to describe molecular mechanisms associated with IL-8 production during HPIV-1 infection and the role of viral replication in MAPK synthesis and activation. An in vitro model of HPIV-1 i...

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Bibliographic Details
Published in:	Journal of immunology research Vol. 2014; no. 2014; pp. 1 - 12
Main Authors:	Valencia Maqueda, Elba, Valle Peralta, Leticia, Mejía Nepomuceno, Fidencio, Cabello Gutiérrez, Carlos, Galván Morales, Miguel Ángel, Manjarrez Zavala, María Eugenia
Format:	Journal Article
Language:	English
Published:	Cairo, Egypt Hindawi Publishing Corporation 01-01-2014 Hindawi Limited
Subjects:	Biomedical research Cell division Cell Line Epithelial Cells - metabolism Epithelial Cells - virology Epithelium - metabolism Epithelium - virology Gene Expression Herpes viruses Humans Immunology Infections Interleukin-8 - biosynthesis Interleukin-8 - genetics JNK Mitogen-Activated Protein Kinases - metabolism Kinases Larynx p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors p38 Mitogen-Activated Protein Kinases - metabolism Parainfluenza virus Parainfluenza Virus 1, Human - physiology Pathogenesis Phosphorylation Protein Kinase Inhibitors - pharmacology Proteins Signal transduction Signal Transduction - drug effects Studies Ultraviolet Rays Virus Inactivation - radiation effects Virus Replication - drug effects
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Summary:	Human parainfluenza virus type 1 (HPIV-1) is the most common cause of croup in infants. The aim of this study was to describe molecular mechanisms associated with IL-8 production during HPIV-1 infection and the role of viral replication in MAPK synthesis and activation. An in vitro model of HPIV-1 infection in the HEp-2 and A549 cell lines was used; a kinetic-based ELISA for IL-8 detection was also used, phosphorylation of the mitogen-activated protein kinases (MAPKs) was identified by Western blot analysis, and specific inhibitors for each kinase were used to identify which MAPK was involved. Inactivated viruses were used to assess whether viral replication is required for IL-8 production. Results revealed a gradual increase in IL-8 production at different selected times, when phosphorylation of MAPK was detected. The secretion of IL-8 in the two cell lines infected with the HPIV-1 is related to the phosphorylation of the MAPK as well as viral replication. Inhibition of p38 suppressed the secretion of IL-8 in the HEp-2 cells. No kinase activation was observed when viruses were inactivated.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Academic Editor: Michael H. Kershaw
ISSN:	2314-8861 2314-7156
DOI:	10.1155/2014/515984