In vitro propagation of mahogany tree (Swietenia macrophylla King) from axillary buds

The mahogany tree (Swietenia macrophylla King) is a forest tree with a great commercial value mainly due to its wood quality. Unfortunately, this species is being threatened by the effect of intensive timber exploitation, its low capacity to regenerate, and the attack of the Meliaceae shoot borer (H...

Full description

Saved in:

Bibliographic Details
Published in:	Acta científica venezolana Vol. 55; no. 1; p. 7
Main Authors:	Tacoronte, Melángel, Vielma, María, Mora, Argenis, Valecillos, Carle
Format:	Journal Article
Language:	Spanish
Published:	Venezuela 2004
Subjects:	Benzyl Compounds Culture Techniques - methods Kinetin - pharmacology Meliaceae - drug effects Meliaceae - growth & development Naphthaleneacetic Acids - pharmacology Plant Growth Regulators - pharmacology Plant Roots - drug effects Plant Roots - growth & development Purines Regeneration - drug effects
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The mahogany tree (Swietenia macrophylla King) is a forest tree with a great commercial value mainly due to its wood quality. Unfortunately, this species is being threatened by the effect of intensive timber exploitation, its low capacity to regenerate, and the attack of the Meliaceae shoot borer (Hypsipylla grandella Zeller) (Lepidoptera Pyralidae). To increase population, large-scale propagation of mahogany by in vitro culture was developed. To obtain plantlets, nodal segments were cultured in a half-strength MS medium supplemented with different combinations of naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), specified by the Central Compositional Rotable Statistical Design Method, within a range of 0-3 mg/L for both hormones. Favourable incubation conditions were: 16 h light, and 40-45 micromol x m(-2) x s(-1) at 25 degrees C. The derived response surface showed an optimal axillary bud elongation on a medium containing 1.94 mg BA/L and 0.38 mg NAA/L. No significant differences were found neither applying Orellana Method nor modified Orellana method. Mahogany plantlets obtained were successfully acclimated.
ISSN:	0001-5504