The Kinetics and Mechanism of Inhibition of Horseradish Peroxidase Activity by N-(Phosphonomethyl)glycine

The Kinetics and Mechanism of Inhibition of Horseradish Peroxidase Activity by N-(Phosphonomethyl)glycine

The inhibition of horseradish peroxidase activity by N-(phosphonomethyl)glycine, the active ingredient of the Roundup herbicide (glyphosate), which is the most common herbicide in agricultural practice in the world, was investigated. It has been shown that N-(phosphonomethyl)glycine inhibited the ac...

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Bibliographic Details
Published in:Biophysics (Oxford) Vol. 68; no. 3; pp. 347 - 355
Main Authors: Avdeeva, L. V., Vakhterova, Ya. V., Saratovskikh, E. A.
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 01-06-2023
Springer Nature B.V
Subjects:
Biological and Medical Physics
Biophysics
Free radicals
Glycine
Glyphosate
Herbicides
Horseradish peroxidase
Molecular Biophysics
Physics
Physics and Astronomy
enzyme inhibition
Roundup (glyphosate)
horseradish peroxidase
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Summary:The inhibition of horseradish peroxidase activity by N-(phosphonomethyl)glycine, the active ingredient of the Roundup herbicide (glyphosate), which is the most common herbicide in agricultural practice in the world, was investigated. It has been shown that N-(phosphonomethyl)glycine inhibited the activity of peroxidase from horseradish roots by a competitive type, K i = 0.1004 mM. Michaelis constant was K m = 1.776 mM and the half-maximal inhibitory concentration was I 50 = 0.302 mM. The data we obtained indicated that N-(phosphonomethyl)glycine can block the work of the antioxidant systems and can cause the accumulation of free radicals in warm-blooded organisms, which is an addition to the available literature data on biological targets of Roundup.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350923030028