Molecular profiling of multidrug-resistant river water isolates: insights into resistance mechanism and potential inhibitors

Polluted waters are an important reservoir for antibiotic resistance genes and multidrug-resistant bacteria. This report describes the microbial community, antibiotic resistance genes, and the genetic profile of extended spectrum β-lactamase strains isolated from rivers at, Pune, India. ESBL-produci...

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Bibliographic Details
Published in:	Environmental science and pollution research international Vol. 27; no. 22; pp. 27279 - 27292
Main Authors:	Yewale, Priti Prabhakar, Lokhande, Kiran Bharat, Sridhar, Aishwarya, Vaishnav, Monika, Khan, Faisal Ahmad, Mandal, Abul, Swamy, Kakumani Venkateswara, Jass, Jana, Nawani, Neelu
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-08-2020 Springer Nature B.V
Subjects:	Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Antimicrobial resistance Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Bacteria Bacterial Proteins Biotechnology Bioteknik Catchments Communities Drug development Drug resistance Drug Resistance, Multiple, Bacterial - drug effects E coli Earth and Environmental Science Ecotoxicology Efflux Efflux pump inhibitors Enterobacter cloacae Environment Environmental Chemistry Environmental Health Environmental science Escherichia coli Extended spectrum β-lactamase Gene sequencing Genes Genomes Genomic analysis Homology India Inhibitors MacB Microbial Sensitivity Tests Microorganisms Molecular docking Molecular Docking Simulation Multidrug efflux pump Multidrug resistance Multidrug resistant organisms Next-generation sequencing Pseudomonas aeruginosa Rivers Selective media Sustainable Industrial and Environmental Bioprocesses Waste Water Technology Water isolates Water Management Water Pollution Control Water resistance India Antimicrobial resistance Efflux pump inhibitors Extended spectrum β-lactamase Multidrug efflux pump MacB
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Summary:	Polluted waters are an important reservoir for antibiotic resistance genes and multidrug-resistant bacteria. This report describes the microbial community, antibiotic resistance genes, and the genetic profile of extended spectrum β-lactamase strains isolated from rivers at, Pune, India. ESBL-producing bacteria isolated from diverse river water catchments running through Pune City were characterized for their antibiotic resistance. The microbial community and types of genes which confer antibiotic resistance were identified followed by the isolation of antibiotic-resistant bacteria on selective media and their genome analysis. Four representative isolates were sequenced using next generation sequencing for genomic analysis. They were identified as Pseudomonas aeruginosa , Escherichia coli , and two isolates were Enterobacter cloacae . The genes associated with the multidrug efflux pumps, such as tolC , macA , macB , adeL , and rosB, were detected in the isolates. As MacAB-TolC is an ABC type efflux pump responsible for conferring resistance in bacteria to several antibiotics, potential efflux pump inhibitors were identified by molecular docking. The homology model of their MacB protein with that from Escherichia coli K12 demonstrated structural changes in different motifs of MacB. Molecular docking of reported efflux pump inhibitors revealed the highest binding affinity of compound MC207-110 against MacB . It also details the potential efflux pump inhibitors that can serve as possible drug targets in drug development and discovery.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0944-1344 1614-7499 1614-7499
DOI:	10.1007/s11356-019-05738-2