Search Results - "Vaimberg, Emma W."

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities by Sanson, Kendall R., Hanna, Ruth E., Hegde, Mudra, Donovan, Katherine F., Strand, Christine, Sullender, Meagan E., Vaimberg, Emma W., Goodale, Amy, Root, David E., Piccioni, Federica, Doench, John G.

“…The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation…”
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Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool by Donovan, Katherine F, Hegde, Mudra, Sullender, Meagan, Vaimberg, Emma W, Johannessen, Cory M, Root, David E, Doench, John G

“…CRISPR/Cas9 screening has proven to be a versatile tool for genomics research. Based on unexpected results from a genome-wide screen, we developed a…”
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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 by Doench, John G, Fusi, Nicolo, Sullender, Meagan, Hegde, Mudra, Vaimberg, Emma W, Donovan, Katherine F, Smith, Ian, Tothova, Zuzana, Wilen, Craig, Orchard, Robert, Virgin, Herbert W, Listgarten, Jennifer, Root, David E

“…Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and…”
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Orthologous CRISPR–Cas9 enzymes for combinatorial genetic screens by Najm, Fadi J, Strand, Christine, Donovan, Katherine F, Hegde, Mudra, Sanson, Kendall R, Vaimberg, Emma W, Sullender, Meagan E, Hartenian, Ella, Kalani, Zohra, Fusi, Nicolo, Listgarten, Jennifer, Younger, Scott T, Bernstein, Bradley E, Root, David E, Doench, John G

“…Combinatorial CRISPR screens are improved by using Cas9 enzymes from two different organisms. Combinatorial genetic screening using CRISPR–Cas9 is a useful…”
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