Identification of differential resistance to six Fusarium oxysporum f. sp. cepae isolates in commercial onion cultivars through the development of a rapid seedling assay

Identification of differential resistance to six Fusarium oxysporum f. sp. cepae isolates in commercial onion cultivars through the development of a rapid seedling assay

Fusarium oxysporum isolates collected from onions in the UK and other countries were characterized using sequences of the transfer elongation factor 1‐α (TEF) gene and compared with published sequence data for 10 other isolates. Isolates associated with diseased onion bulbs in the UK formed two clad...

Full description

Saved in:
Bibliographic Details
Published in:Plant pathology Vol. 62; no. 1; pp. 103 - 111
Main Authors: Taylor, A, Vagany, V, Barbara, D. J, Thomas, B, Pink, D. A. C, Jones, J. E, Clarkson, J. P
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01-02-2013
Blackwell
Wiley Subscription Services, Inc
Subjects:
aggression
Agronomy. Soil science and plant productions
Allium cepa
Basal rot
Biological and medical sciences
Bulb rot
Bulbs
cultivars
Data processing
Elongation
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
fusarium basal rot
Fusarium oxysporum
Fusarium oxysporum f. sp. cepae
genes
Genetics and breeding of economic plants
onion
onions
Pathogenicity
Phytopathology. Animal pests. Plant and forest protection
seedling assay
Seedlings
tef gene
United Kingdom
Monocotyledones
Plant pathology
Plant pathogen
Plant juvenile growth stage
onion
Identification
Fungi
Assay
cepae
Rapid technique
Angiospermae
seedling assay
Development
Developmental stage
Fungi Imperfecti
sp
Allium cepa
Fusarium oxysporum
fusarium basal rot
Resistance
Fusarium oxysporum f
Liliaceae
Vegetable crop
Rot
Isolate
Spermatophyta
Cultivar
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fusarium oxysporum isolates collected from onions in the UK and other countries were characterized using sequences of the transfer elongation factor 1‐α (TEF) gene and compared with published sequence data for 10 other isolates. Isolates associated with diseased onion bulbs in the UK formed two clades. Isolates from both clades were selected for pathogenicity testing and to develop a rapid seedling assay to screen commercial onion cultivars for resistance to F. oxysporum f. sp. cepae (FOC), the cause of basal rot. Differences in the levels of aggressiveness between isolates were observed and isolates from both clades were pathogenic. Differences in resistance/susceptibility were also observed amongst 10 commercial onion cultivars, with cvs Ailsa Craig Prizewinner and White Lisbon showing the highest levels of resistance. The results from the seedling assay were supported by those from a subsequent onion bulb rot assay. Thus, this study reports the development of a rapid, simple and repeatable seedling assay that can be used to screen large numbers of onion cultivars for resistance to FOC and which is indicative of resistance at the bulb stage.
Bibliography:http://dx.doi.org/10.1111/j.1365-3059.2012.02624.x
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0032-0862
1365-3059
DOI:10.1111/j.1365-3059.2012.02624.x