Microbial community composition in Brazilian stored diesel fuel of varying sulfur content, using high-throughput sequencing

•The highest interfacial biomass was detected in UHSD during simulated storage.•Firmicutes, Bacteroidetes, Actinobacteria and Ascomycota were the most abundant phyla.•Bacterial diversity is inversely proportional to the sulfur content in diesel fuel.•NMR spectrosc...

Full description

Saved in:

Bibliographic Details
Published in:	Fuel (Guildford) Vol. 189; pp. 340 - 349
Main Authors:	de Azambuja, Aline Oliboni, Bücker, Francielle, de Quadros, Patrícia Dörr, Zhalnina, Kateryna, Dias, Raquel, Vacaro, Bernardo Brito, Correa, Camila, Ferrão, Marco Flôres, de Oliveira Camargo, Flávio Anastácio, Triplett, Eric, Bento, Fátima Menezes
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 01-02-2017 Elsevier BV
Subjects:	Ascomycota Biomass Bioremediation Chain branching Communities Community composition Community structure Contamination Control methods Diesel Diesel fuels Environmental impact Flasks Fuel tanks Fungi High-throughput sequencing analysis Microbial community Microbial contamination Microbiomes Microcosms Microorganisms Next-generation sequencing NMR Nuclear fuels Nuclear magnetic resonance Reduction rRNA 16S Spores Storage Storage conditions Storage tanks Sulfur Sulfur content ULSD Sulfur content Storage High-throughput sequencing analysis Microbial community ULSD
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	•The highest interfacial biomass was detected in UHSD during simulated storage.•Firmicutes, Bacteroidetes, Actinobacteria and Ascomycota were the most abundant phyla.•Bacterial diversity is inversely proportional to the sulfur content in diesel fuel.•NMR spectroscopy revealed higher branching in the hydrocarbon chains of ULSD.•Reduction of sulfur in diesel fuel per se do not increase microbial contamination. The reduction of sulfur content in diesel is a worldwide trend that seeks to minimize the environmental impact arising from the combustion of this fuel in large cities. However, there is no reliable information on the effect of such a reduction on the formation of mainly aerobic microbial biomass in vehicle and storage tanks. The objective of this study was to assess the microbial community structure using next generation sequencing technique, and to evaluate the microbial biomass in pure diesel containing various sulfur concentrations. Storage conditions were simulated in glass flasks containing mineral medium and pure diesel, with varying sulfur content: UHSD (⩽1800ppm sulfur); HSD (⩽500ppm sulfur); LSD (⩽50ppm sulfur) and ULSD (⩽10ppm sulfur). Were assessed two microbial contamination levels in the diesel: low contamination (∼103CFUL−1) and medium contamination (∼105 bacterial cells and fungal spores mL−1). The flasks were incubated at 30°C and monitored every 10days over 40days. Microbial biomass formation was observed in all microcosms, but the highest biomass level at all times was in UHSD with medium contamination (p<0.05). Results from biomass production and proton nuclear magnetic resonance showed that the ULSD, was not prone to microbial contamination probably because has highly branched carbon chain. Overall, 36 different phyla were identified by sequencing of 16S rDNA and four by 18S rDNA, with Firmicutes (∼49%), Bacteroidetes (∼25%), Actinobacteria (∼17%) and Ascomycota (>99%) being the most abundant. Results suggested that bacterial diversity is increased when low sulfur concentrations are present. Our results reveal a complex microbial diversity from 16S rDNA and confirm the existence of the major fungal Phyla (Ascomycota, Basidiomycota, Chytridiomycota and Glomeromycota) with a large fraction of microorganisms as yet undescribed, reinforcing the importance of understanding the fuel environment in establishing control methods of microbial growth in storage tanks and in bioremediation programs for environments contaminated by fuel.
ISSN:	0016-2361 1873-7153
DOI:	10.1016/j.fuel.2016.10.108