Quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice (Oryza sativa)

Blast is an economically important disease of rice. To map genes controlling blast resistance, recombinant inbred lines (RIL) were developed from Khao Dawk Mali 105, an aromatic, blast-susceptible cultivar and the blast resistance donor, CT 9993-5-10-M (CT). A linkage map encompassing 2112 cM was co...

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Bibliographic Details
Published in:	DNA research Vol. 9; no. 3; pp. 79 - 88
Main Authors:	SIRITHUNYA, Pattama, TRAGOONRUNG, Somvong, VANAVICHIT, Apichart, PA-IN, Nathinee, VONGSAPROM, Chanakarn, TOOJINDA, Theerayut
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 30-06-2002
Subjects:	Biological and medical sciences Chromosome Mapping Chromosomes - genetics Classical genetics, quantitative genetics, hybrids Crosses, Genetic DNA, Plant Fundamental and applied biological sciences. Psychology Genes, Plant Genetic Linkage Genetics of eukaryotes. Biological and molecular evolution Genome, Plant Genotype Immunity, Innate Oryza - genetics Oryza sativa Phenotype Plant Diseases - genetics Plant Leaves Polymorphism, Restriction Fragment Length Pteridophyta, spermatophyta Quantitative Trait, Heritable Recombination, Genetic Vegetals Thailand Monocotyledones Quantitative character Cereal crop Quantitative trait loci Resistance Oryza sativa Gramineae Angiospermae Spermatophyta Inbred Recombinant protein Polymorphism Rice
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Summary:	Blast is an economically important disease of rice. To map genes controlling blast resistance, recombinant inbred lines (RIL) were developed from Khao Dawk Mali 105, an aromatic, blast-susceptible cultivar and the blast resistance donor, CT 9993-5-10-M (CT). A linkage map encompassing 2112 cM was constructed from 141 RILs using 90 restriction fragment length polymorphisms (RFLPs) and 31 simple sequence repeats (SSR). Virulent isolates of blast fungus were identified by screening differential host sets against 87 single-spore isolates collected from the north and northeast of Thailand. Fifteen virulent blast isolates were selected for leaf blast screening. Neck blast was evaluated both under natural conditions and controlled inoculations. Quantitative trait loci (QTLs) for broad resistance spectrum (BRS) to leaf blast were located on chromosomes 7 and 9. In particular, the QTL(ch9) was mapped near the Pi5(t) locus. The QTL(ch7) was located close to a previously mapped partial resistance QTL. Both loci showed significant allelic interaction. Genotypes having CT alleles at both QTL(ch7) and QTL(ch9) were the most resistant. Two neck-blast QTLs were mapped on chromosomes 5 and 6. The inconsistent map locations between the leaf and neck blast QTLs indicate the complexity of fixing both leaf and neck blast resistance. The coincidence of BRS and field resistance QTLs on chromosome 7 supports the idea that BRS may reflect the broad resistance spectrum to leaf blast in rice. These findings laid the foundation for the development of a marker-assisted scheme for improving Khoa Dawk Mali 105 and the majority of aromatic Thai rice varieties that are susceptible to blast.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1340-2838 1756-1663
DOI:	10.1093/dnares/9.3.79