Search Results - "VITZTHUM, Frank"

HUPO Plasma Proteome Project specimen collection and handling: Towards the standardization of parameters for plasma proteome samples by Rai, Alex J., Gelfand, Craig A., Haywood, Bruce C., Warunek, David J., Yi, Jizu, Schuchard, Mark D., Mehigh, Richard J., Cockrill, Steven L., Scott, Graham B. I., Tammen, Harald, Schulz-Knappe, Peter, Speicher, David W., Vitzthum, Frank, Haab, Brian B., Siest, Gerard, Chan, Daniel W.

“…There is a substantial list of pre‐analytical variables that can alter the analysis of blood‐derived samples. We have undertaken studies on some of these…”
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Plasma factor VII-activating protease is increased by oral contraceptives and induces factor VII activation in-vivo by Sidelmann, Johannes J, Skouby, Sven O, Kluft, Cornelis, Winkler, Ulrich, Vitzthum, Frank, Schwarz, Herbert, Gram, Jørgen, Jespersen, Jørgen

“…Abstract Oral contraceptive (OC) use influences the hemostatic system significantly and is a risk factor for development of cardiovascular disease. Factor…”
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A Quantitative Fluorescence-Based Microplate Assay for the Determination of Double-Stranded DNA Using SYBR Green I and a Standard Ultraviolet Transilluminator Gel Imaging System by Vitzthum, Frank, Geiger, Georg, Bisswanger, Hans, Brunner, Herwig, Bernhagen, Jürgen

“…Various assays are available for quantification of DNA in solution, but none has been described that is both sensitive and specific for double-stranded (ds)…”
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Treatment of simulated wastewater containing toxic amides by immobilized Rhodococcus rhodochrous NHB-2 using a highly compact 5-stage plug flow reactor by CHAND, Duni, KUMAR, Harish, UMA DUTT SANKHIAN, KUMAR, Dinesh, VITZTHUM, Frank, TEK CHAND BHALLA

“…Biodegradation of toxic amides by immobilized Rhodococcus rhodochrous NHB-2 has been studied to generate data for future development of reactors for the…”
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Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications by Zipper, Hubert, Brunner, Herwig, Bernhagen, Jürgen, Vitzthum, Frank

“…The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA…”
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Effects of preanalytical variables on peptide and protein measurements in human serum and plasma: implications for clinical proteomics by Rai, Alex J, Vitzthum, Frank

“…There is a wealth of knowledge in the field of in vitro diagnostics with regard to preanalytical variables and their impact on the determination of peptide and…”
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Development of High-Throughput Chemical Extraction Techniques and Quantitative HPLC-MS/MS (SRM) Assays for Clinically Relevant Plasma Proteins by Barton, Chris, Kay, Richard G, Gentzer, Wolfgang, Vitzthum, Frank, Pleasance, Steve

“…The clinical application of targeted plasma protein analysis by selective reaction monitoring of peptides using LC-MS/MS requires the development of robust,…”
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Proteomics:  From Basic Research to Diagnostic Application. A Review of Requirements & Needs by Vitzthum, Frank, Behrens, Fritz, Anderson, N. Leigh, Shaw, John H

“…For several years proteomics research has been expected to lead to the finding of new markers that will translate into clinical tests applicable to samples…”
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Factor VII-activating protease: sex-related association with coronary artery calcification by Ramanathan, Ramshanker, Gram, Jørgen B, Sand, Niels Peter R, Nørgaard, Bjarne L, Diederichsen, Axel C P, Vitzthum, Frank, Schwarz, Herbert, Sidelmann, Johannes J

“…: Factor VII-activating protease (FSAP) may regulate development of cardiovascular disease (CVD). We evaluated sex differences in FSAP measures and examined…”
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Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens: Systematic variation between sample types and calibration of mass spectrometry data by Haab, Brian B., Geierstanger, Bernhard H., Michailidis, George, Vitzthum, Frank, Forrester, Sara, Okon, Ryan, Saviranta, Petri, Brinker, Achim, Sorette, Martin, Perlee, Lorah, Suresh, Shubha, Drwal, Garry, Adkins, Joshua N., Omenn, Gilbert S.

“…Four different immunoassay and antibody microarray methods performed at four different sites were used to measure the levels of a broad range of proteins (N =…”
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Mechanisms underlying the impact of humic acids on DNA quantification by SYBR Green I and consequences for the analysis of soils and aquatic sediments by Zipper, Hubert, Buta, Christiane, Lämmle, Katrin, Brunner, Herwig, Bernhagen, Jürgen, Vitzthum, Frank

“…DNA quantification of soils and sediments is useful for the investigation of microbial communities and for the acquisition of their genomes that are exploited…”
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Tests for the measurement of factor VII-activating protease (FSAP) activity and antigen levels in citrated plasma, their correlation to PCR testing, and utility for the detection of the Marburg I-polymorphism of FSAP by Stephan, Sina, Schwarz, Herbert, Borchert, Anja, Bussfeld, Delia, Quak, Elfriede, Simshaeuser-Knaub, Beate, Teigelkamp, Stefan, Behrens, Fritz, Vitzthum, Frank

“…The single nucleotide Marburg I (MRI) polymorphism of the factor VII-activating protease (FSAP) gene, the prourokinase-activating activity of FSAP, and antigen…”
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Quantification of coagulation factor XIII activity by a thio-NADH based assay using factor XIII immuno-depleted plasma as a diluent for calibration by Kappel, Andreas, Stephan, Sina, Christ, Gerlinde, Pechmann, Lena, Duwe, Christa, Fischer, Bodo, Hahn, Martin, Althaus, Harald, Ehm, Matthias, Schwarz, Herbert, Vitzthum, Frank

“…Accurate determination of factor XIII (FXIII) activity is crucial for replacement therapy. FXIII activity is typically determined using a coupled enzymatic…”
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Factor VII-activating protease in patients with acute deep venous thrombosis by Sidelmann, Johannes J, Vitzthum, Frank, Funding, Eva, Münster, Anna-Marie B, Gram, Jørgen, Jespersen, Jørgen

“…Abstract Factor VII-activating protease (FSAP) is involved in haemostasis and inflammation. FSAP cleaves single chain urokinase-type plasminogen activator…”
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Direct chromogenic substrate immuno-capture activity assay for testing of factor VII-activating protease by Stephan, Sina, Schwarz, Herbert, Haude-Barten, Anja, Sidelmann, Johannes J, Fischer, Bodo, Althaus, Harald, Hahn, Martin, Kappel, Andreas, Ehm, Matthias, Vitzthum, Frank

“…The Marburg I (MRI) single nucleotide polymorphism (SNP) of the factor VII-activating protease (FSAP) gene has been associated with thrombophilia and…”
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Qualitative detection of the Marburg I alloenzyme of factor VII-activating protease by an immunoassay and its comparison to PCR testing by Schwarz, Herbert, Stephan, Sina, Sidelmann, Johannes J, Fischer, Bodo, Althaus, Harald, Hahn, Martin, Kappel, Andreas, Ehm, Matthias, Vitzthum, Frank

“…The Marburg I (MRI) single nucleotide polymorphism (SNP) of the factor VII-activating protease (FSAP) gene has been associated with thrombophilia,…”
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A proteomic study of the HUPO Plasma Proteome Project's pilot samples using an accurate mass and time tag strategy by Adkins, Joshua N., Monroe, Matthew E., Auberry, Kenneth J., Shen, Yufeng, Jacobs, Jon M., Camp II, David G., Vitzthum, Frank, Rodland, Karin D., Zangar, Richard C., Smith, Richard D., Pounds, Joel G.

“…Characterization of the human blood plasma proteome is critical to the discovery of routinely useful clinical biomarkers. We used an accurate mass and time…”
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Coagulation assays based on the Luminescent Oxygen Channeling Immunoassay technology 1 by Kappel, Andreas, Stephan, Sina, Christ, Gerlinde, Haude-Barten, Anja, Dahm, Madeleine, Schwarz, Herbert, Fischer, Bodo, Hahn, Martin, Althaus, Harald, Ehm, Matthias, Vitzthum, Frank

“…The Luminescent Oxygen Channeling Immunoassay (LOCI) technology is a well-established homogeneous assay format that allows for fast, accurate, and highly…”
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A Proteomic Study of HUPO’s Plasma Proteome Project Pilot Samples using an Accurate Mass and Time Tag Strategy by Adkins, Joshua N., Monroe, Matthew E., Auberry, Kenneth J., Shen, Yufeng, Jacobs, Jon M., Camp, David G., Vitzthum, Frank, Rodland, Karin D., Smith, Richard D., Pounds, Joel G.

“…Characterization of the human blood plasma proteome is critical to the discovery of routinely useful clinical biomarkers. We used an Accurate Mass and Time…”
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Metrological sharp shooting for plasma proteins and peptides: The need for reference materials for accurate measurements in clinical proteomics and in vitro diagnostics to generate reliable results by Vitzthum, Frank, Siest, Gérard, Bunk, David M., Preckel, Tobias, Wenz, Christian, Hoerth, Patric, Schulz-Knappe, Peter, Tammen, Harald, Adamkiewicz, Juergen, Merlini, Giampaolo, Anderson, N. Leigh

“…Reliable study results are necessary for the assessment of discoveries, including those from proteomics. Reliable study results are also crucial to increase…”
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