Search Results - "VIDUGIRIENE, J"
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187 Bioluminescent Assays to Monitor NAD(P)/NAD(P)H Levels and Reactive Oxygen Species in Live Cells
Published in European journal of cancer (1990) (01-11-2012)Get full text
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Characterization of Caveolin-Rich Membrane Domains Isolated from an Endothelial-Rich Source: Implications for Human Disease
Published in The Journal of cell biology (01-07-1994)“…Caveolae are 50-100-nm membrane microdomains that represent a subcompartment of the plasma membrane. Previous morphological studies have implicated caveolae in…”
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Miniaturized GPCR signaling studies in 1536-well format
Published in Journal of biomolecular techniques (01-09-2008)“…G protein-coupled receptors (GPCRs) are involved in various physiological processes, such as behavior changes, mood alteration, and regulation of immune-system…”
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Early Lipid Intermediates in Glycosyl-Phosphatidylinositol Anchor Assembly Are Synthesized in the ER and Located in the Cytoplasmic Leaflet of the ER Membrane Bilayer
Published in The Journal of cell biology (01-06-1993)“…Glycosylated phosphoinositides serve as membrane anchors for numerous eukaryotic cell surface glycoproteins. Recent biochemical and genetic studies indicate…”
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The GPI Anchor of Cell-Surface Proteins Is Synthesized on the Cytoplasmic Face of the Endoplasmic Reticulum
Published in The Journal of cell biology (01-10-1994)“…Glycosylphosphatidylinositol (GPI) membrane protein anchors are synthesized from sugar nucleotides and phospholipids in the ER and transferred to newly…”
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Soluble constituents of the ER lumen are required for GPI anchoring of a model protein
Published in The EMBO journal (02-10-1995)“…Transfer of a glycosylphosphatidylinositol (GPI) anchor to proteins carrying a C‐terminal GPI‐directing signal sequence occurs after protein translocation…”
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Glycosylphosphatidylinositols: biosynthesis and intracellular transport
Published in Biochemical Society transactions (01-08-1997)Get more information
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Topology of GPI biosynthesis in the endoplasmic reticulum
Published in Brazilian journal of medical and biological research (01-02-1994)“…Glycosylphosphatidylinositol (GPI) anchors are constructed in the endoplasmic reticulum (ER) through the action of at least seven unique enzymes. Using…”
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Phosphatidylinositol 4-OH Kinase Is a Downstream Target of Neuronal Calcium Sensor-1 in Enhancing Exocytosis in Neuroendocrine Cells
Published in The Journal of biological chemistry (21-02-2003)“…Neuronal calcium sensor-1 (NCS-1), the mammalian orthologue of frequenin, belongs to a family of EF-hand-containing Ca 2+ sensors. NCS-1/frequenin has been…”
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Evaluating the utility of a bioluminescent ADP-detecting assay for lipid kinases
Published in Assay and drug development technologies (01-12-2009)“…The lipid second messengers phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) and sphingosine 1-phosphate (S1P) are well recognized to play important…”
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Neuronal calcium sensor‐1 facilitates neuronal exocytosis through phosphatidylinositol 4‐kinase
Published in Journal of neurochemistry (01-02-2005)“…This work tested the theory that neuronal calcium sensor‐1 (NCS‐1) has effects on neurotransmitter release beyond its actions on membrane channels. We used…”
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A bioluminescent-based, HTS-compatible assay to monitor G-protein-coupled receptor modulation of cellular cyclic AMP
Published in Assay and drug development technologies (01-04-2007)“…We have developed a novel assay for monitoring changes in intracellular cyclic AMP (cAMP) concentration with high sensitivity (30 +/- 5 fmol [mean +/- standard…”
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cell-free assay for glycosylphosphatidylinositol anchoring in African trypanosomes. Demonstration of a transamidation reaction mechanism
Published in The Journal of biological chemistry (04-06-1999)“…We established an in vitro assay for the addition of glycosyl-phosphatidylinositol (GPI) anchors to proteins using procyclic trypanosomes engineered to express…”
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Influenza A H1N1: A Rapid Approach to Cloning and Protein Expression
Published in Journal of biomolecular techniques (01-09-2010)“…RP-88 In May 2009, Swine-derived Influenza A H1N1 virus emerged as a serious threat to global health. With the threat came the need for the virology community…”
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Segregation of Glycosylphosphatidylinositol Biosynthetic Reactions in a Subcompartment of the Endoplasmic Reticulum
Published in The Journal of biological chemistry (21-05-1999)“…Glycosylphosphatidylinositols (GPIs) are synthesized in the endoplasmic reticulum (ER) via the sequential addition of monosaccharides, fatty acid, and…”
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Cell Surface Display and Intracellular Trafficking of Free Glycosylphosphatidylinositols in Mammalian Cells
Published in The Journal of biological chemistry (10-03-2000)“…In addition to serving as membrane anchors for cell surface proteins, glycosylphosphatidylinositols (GPIs) can be found abundantly as free glycolipids in…”
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Endoplasmic reticulum proteins involved in glycosylphosphatidylinositol-anchor attachment: photocrosslinking studies in a cell-free system
Published in European journal of biochemistry (01-04-2001)“…Assembly of glycosylphosphatidylinositol (GPtdIns)-anchored proteins requires translocation of the nascent polypeptide chain across the endoplasmic reticulum…”
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Biosynthesis of glycosylphosphatidylinositol anchors
Published in Methods in enzymology (1995)Get more information
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Soluble constituents of the ER lumen are required for GPI anchoring of a model protein
Published in Parasitology today (Regular ed.) (01-12-1995)Get full text
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On protein translocation across bacterial cytoplasmic membranes
Published in Antonie van Leeuwenhoek (01-02-1992)Get full text
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