Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes

Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes

Substrate elasticity or stiffness can influence the phenotypic and functional characteristics of chondrocytes. This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and func...

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Bibliographic Details
Published in:International journal of biological macromolecules Vol. 104; no. Pt B; pp. 1925 - 1935
Main Authors: V Thomas, Lynda, VG, Rahul, D Nair, Prabha
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-11-2017
Subjects:
Animals
Articular chondrocytes
Biocompatible Materials
Cartilage, Articular - cytology
Cell Survival
Chitosan
Chitosan - chemistry
Chondrocytes - cytology
Glycosaminoglycans - chemistry
Hyaluronic acid
Hyaluronic Acid - chemistry
Hydrogels - chemistry
Injectable hydrogels
Mechanical Phenomena
Microscopy, Confocal
Rabbits
Spectroscopy, Fourier Transform Infrared
Substrate stiffness
Thermogravimetry
Tissue Scaffolds
Substrate stiffness
Injectable hydrogels
Chitosan
Hyaluronic acid
Articular chondrocytes
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Summary:Substrate elasticity or stiffness can influence the phenotypic and functional characteristics of chondrocytes. This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes. Three different ratios of the gel were prepared (10:1,10:3 and 10:5 CH-HDA) and characterized. The stiffness of the gels was evaluated from the force displacement curves using force spectroscopy AFM analysis. Rabbit articular chondrocytes were harvested and the cells from Passage 2 to 4 were used for the encapsulation study. The viability and ECM production of encapsulated chondrocytes were assessed at 7day, 14day and 28day post culture. The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time. Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels. These findings indicate that gel stiffness strongly impacts the chondrocyte microenvironment both in maintenance of phenotypic integrity and ECM production.
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content type line 23
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2017.05.116