Search Results - "VERNèDE, Xavier"

Structural Insights into the Mechanism of the Radical SAM Carbide Synthase NifB, a Key Nitrogenase Cofactor Maturating Enzyme by Fajardo, Ana Sosa, Legrand, Pierre, Payá-Tormo, Lucı́a, Martin, Lydie, Pellicer Martı́nez, Maria Teresa, Echavarri-Erasun, Carlos, Vernède, Xavier, Rubio, Luis M, Nicolet, Yvain

“…Nitrogenase is a key player in the global nitrogen cycle, as it catalyzes the reduction of dinitrogen into ammonia. The active site of the nitrogenase MoFe…”
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Oxygen-Sensitive Metalloprotein Structure Determination by Cryo-Electron Microscopy by Cherrier, Mickaël V, Vernède, Xavier, Fenel, Daphna, Martin, Lydie, Arragain, Benoit, Neumann, Emmanuelle, Fontecilla-Camps, Juan C, Schoehn, Guy, Nicolet, Yvain

“…Metalloproteins are involved in key cell processes such as photosynthesis, respiration, and oxygen transport. However, the presence of transition metals…”
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Advances in spectroscopic methods for biological crystals. 1. Fluorescence lifetime measurements by Royant, Antoine, Carpentier, Philippe, Ohana, Jérémy, McGeehan, John, Paetzold, Bernhard, Noirclerc-Savoye, Marjolaine, Vernède, Xavier, Adam, Virgile, Bourgeois, Dominique

“…Synchrotrons are now producing thousands of macromolecular structures each year. The need for complementary techniques available on site has progressively…”
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Crystal Structure of the Free Radical Intermediate of Pyruvate: Ferredoxin Oxidoreductase by Chabrière, Eric, Vernède, Xavier, Guigliarelli, Bruno, Charon, Marie-Hélène, Hatchikian, E. Claude, Fontecilla-Camps, Juan C.

“…In anaerobic organisms, the decarboxylation of pyruvate, a crucial component of intermediary metabolism, is catalyzed by the metalloenzyme pyruvate: ferredoxin…”
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High-resolution crystallographic analysis of Desulfovibrio fructosovorans [NiFe] hydrogenase by Volbeda, Anne, Montet, Yaël, Vernède, Xavier, Hatchikian, E.Claude, Fontecilla-Camps, Juan C.

“…Two 1.8 A ̊ resolution crystal structures of an oxidised form of the [NiFe] hydrogenase of Desulfovibrio ( D.) fructosovorans are reported. The high data…”
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Methods to Screen for Radical SAM Enzyme Crystallization Conditions by Martin, Lydie, Vernède, Xavier, Nicolet, Yvain

“…Radical S-adenosyl-L-methionine proteins most probably belong to the widest superfamily of metalloenzymes. Thanks to their ability to catalyze difficult…”
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REACH: Robotic Equipment for Automated Crystal Harvesting using a six-axis robot arm and a micro-gripper by Heidari Khajepour, Mohammad Yaser, Vernede, Xavier, Cobessi, David, Lebrette, Hugo, Rogues, Pierrick, Terrien, Maxime, Berzin, Christophe, Ferrer, Jean-Luc

“…In protein crystallography experiments, only two critical steps remain manual: the transfer of crystals from their original crystallization drop into the…”
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Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase by Darnault, Claudine, Volbeda, Anne, Kim, Eun Jin, Legrand, Pierre, Vernède, Xavier, Lindahl, Paul A, Fontecilla-Camps, Juan C

“…The crystal structure of the tetrameric alpha2beta2 acetyl-coenzyme A synthase/carbon monoxide dehydrogenase from Moorella thermoacetica has been solved at 1.9…”
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UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops by Vernede, Xavier, Lavault, Bernard, Ohana, Jeremy, Nurizzo, Didier, Joly, Jacques, Jacquamet, Lilian, Felisaz, Franck, Cipriani, Florent, Bourgeois, Dominique

“…Structural proteomics has promoted the rapid development of automated protein structure determination using X‐ray crystallography. Robotics are now routinely…”
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Ni-Zn-[Fe 4 -S 4 ] and Ni-Ni-[Fe 4 -S 4 ] clusters in closed and open α subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase by Fontecilla-Camps, Juan C, Darnault, Claudine, Volbeda, Anne, Kim, Eun Jin, Legrand, Pierre, Vernède, Xavier, Lindahl, Paul A

“…The crystal structure of the tetrameric alpha sub(2) beta sub(2) acetylcoenzyme A synthase/carbon monoxide dehydrogenase from Moorella thermoacetica has been…”
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Crystallographic and FTIR Spectroscopic Evidence of Changes in Fe Coordination Upon Reduction of the Active Site of the Fe-Only Hydrogenase from Desulfovibrio desulfuricans by NICOLET, Yvain, DE LACEY, Antonio L., VERNèDE, Xavier, FERNANDEZ, Victor M., HATCHIKIAN, E. Claude, FONTECILLA-CAMPS, Juan C.

“…Fe-only hydrogenases, as well as their NiFe counterparts, display unusual intrinsic high-frequency IR bands that have been assigned to CO and CN(-) ligation to…”
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A microspectrophotometer for UV-visible absorption and fluorescence studies of protein crystals by Bourgeois, Dominique, Vernede, Xavier, Adam, Virgile, Fioravanti, Emanuela, Ursby, Thomas

“…Absorption microspectrophotometry has been shown to be of considerable help to probe crystalline proteins containing chromophores, metal centres, or coloured…”
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Gas access to the active site of Ni-Fe hydrogenases probed by X-ray crystallography and molecular dynamics by Montet, Yaël, Amara, Patricia, Volbeda, Anne, Vernede, Xavier, Hatchikian, E. Claude, Field, Martin J, Frey, Michel, Fontecilla-Camps, Juan C

“…The 2.54 A resolution structure of Ni-Fe hydrogenase has revealed the existence of hydrophobic channels connecting the molecular surface to the active site. A…”
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Crystal structure of Escherichia coli UvrB C-terminal domain, and a model for UvrB-UvrC interaction by Sohi, Maninder, Alexandrovich, Alexander, Moolenaar, Geri, Visse, Rob, Goosen, Nora, Vernede, Xavier, Fontecilla-Camps, Juan C., Champness, John, Sanderson, Mark R.

“…A crystal structure of the C-terminal domain of Escherichia coli UvrB (UvrB′) has been solved to 3.0 Å resolution. The domain adopts a helix-loop-helix fold…”
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Automated robot-based systems for crystallography on beamlines and in laboratories, and other developments performed on FIP-BM30A at the ESRF by Ferrer, Jean-Luc, Vernede, Xavier, Sallaz-Damaz, Yoann, Berzin, Christophe, Jacquet, Philippe, Budayova-Spano, Monika, Cobessi, David, Borel, Franck

“…Abstract only…”
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A method to stabilize reduced and/or gas-treated protein crystals by flash-cooling under a controlled atmosphere by VERNEDE, X, FONTECILLA-CAMPS, J. C

“…A customized glove box for protein crystallization under a controlled atmosphere is described along with a cryogenic technique adapted to freeze protein…”
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FIP-BM30A at the ESRF: an automated beamline for protein crystallography with unique features by Ferrer, Jean-Luc, Sallaz-Damaz, Yoann, Vernede, Xavier, Pirocchi, Michel, Berzin, Christophe, Budayova-Spano, Monika, Israel-Gouy, Pascale, Borel, Franck, Cobessi, David

“…Abstract only…”
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Progress of robotized systems for crystallography for beamlines and laboratories by Ferrer, Jean-Luc, Vernede, Xavier, Sallaz-Damaz, Yoann, Berzin, Christophe, Pirocchi, Michel, Borel, Franck, Larive, Nathalie

“…Abstract only Development of 6-axis robotic arm based systems for protein crystallography automation is now expending rapidly. From the seminal work…”
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A geometrical approach for semi-automated crystal centering and in situ X-ray diffraction data collection by Heidari Khajepour, Mohammad Yaser, Lebrette, Hugo, Vernede, Xavier, Rogues, Pierrick, Ferrer, Jean-Luc

“…High‐throughput protein crystallography projects pushed forward the development of automated crystallization platforms that are now commonly used. This created…”
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Simultaneous Measurements of Solvent Dynamics and Functional Kinetics in a Light-Activated Enzyme by Durin, Guillaume, Delaunay, Aude, Darnault, Claudine, Heyes, Derren J., Royant, Antoine, Vernede, Xavier, Hunter, C. Neil, Weik, Martin, Bourgeois, Dominique

“…Solvent fluctuations play a key role in controlling protein motions and biological function. Here, we have studied how individual steps of the reaction…”
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