Search Results - "VERHAERT, Raymond M. D"

Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila by Bonzom, Cyrielle, Hüttner, Silvia, Mirgorodskaya, Ekaterina, Chong, Sun-Li, Uthoff, Stefan, Steinbüchel, Alexander, Verhaert, Raymond M. D., Olsson, Lisbeth

“…Heterologous protein production is widely used in industrial biotechnology. However, using non-native production hosts can lead to enzymes with altered…”
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Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia pastoris by Gidijala, Loknath, Uthoff, Stefan, van Kampen, Sebastiaan J, Steinbüchel, Alexander, Verhaert, Raymond M D

“…The yeast Komagataella phaffii, better known as Pichia pastoris, is a commonly used host for recombinant protein production. Here expression vectors are…”
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The Aspergillus niger multicopper oxidase family: analysis and overexpression of laccase-like encoding genes by Ramos, Juan A Tamayo, Barends, Sharief, Verhaert, Raymond M D, de Graaff, Leo H

“…Many filamentous fungal genomes contain complex groups of multicopper oxidase (MCO) coding genes that makes them a good source for new laccases with potential…”
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Peptide display on live MS2 phage: restrictions at the RNA genome level by van Meerten, Dico, Olsthoorn, Rene C. L, van Duin, Jan, Verhaert, Raymond M. D

“…Leiden Institute of Chemistry, Department of Biochemistry, Gorlaeus Laboratories, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands 1 Author for…”
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Phage display selects for amylases with improved low pH starch-binding by Verhaert, Raymond M.D, Beekwilder, Jules, Olsthoorn, René, van Duin, Jan, Quax, Wim J

“…Directed evolution of secreted industrial enzymes is hampered by the lack of powerful selection techniques. We have explored surface display to select for…”
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Binding of phage displayed Bacillus subtilis lipase A to a phosphonate suicide inhibitor by DRÖGE, Melloney J, RÜGGEBERG, Carsten J, VAN DER SLOOT, Almer M, SCHIMMEL, Judith, DIJKSTRA, Dolf Swaving, VERHAERT, Raymond M. D, REETZ, Manfred T, QUAX, Wim J

“…Phage display can be used as a protein engineering tool to select proteins with desirable binding properties from a library of randomly constructed mutants…”
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Processing and functional display of the 86 kDa heterodimeric penicillin G acylase on the surface of phage fd by VERHAERT, Raymond M. D., DUIN, Jan VAN, QUAX, Wim J.

“…The large heterodimeric penicillin G acylase from Alcaligenes faecalis was displayed on the surface of phage fd. We fused the coding sequence (α…”
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Description of enzyme kinetics in reversed micelles by VERHAERT, Raymond M. D., HILHORST, Riet, VERMUË, Marian, SCHAAFSMA, Tjeerd J., VEEGER, Cees

“…In the literature measurements of kinetic data of enzymes in reversed micelles have been interpreted in two ways. In the first, all enzyme parameters are…”
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Molecular cloning and analysis of the gene encoding the thermostable penicillin G acylase from Alcaligenes faecalis by VERHAERT, R. M. D, RIEMENS, A. M, VAN DER LAAN, J.-M, VAN DUIN, J, QUAX, A. W. J

“…Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon…”
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Directed evolution of a glutaryl acylase into an adipyl acylase by Sio, Charles F., Riemens, Anja M., van der Laan, Jan‐Metske, Verhaert, Raymond M.D., Quax, Wim J.

“…Semi‐synthetic cephalosporin antibiotics belong to the top 10 of most sold drugs, and are produced from 7‐aminodesacetoxycephalosporanic acid (7‐ADCA)…”
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Description of enzyme kinetics in reversed micelles. 1. Theory by Verhaert, R M, Hilhorst, R, Vermuë, M, Schaafsma, T J, Veeger, C

“…In the literature measurements of kinetic data of enzymes in reversed micelles have been interpreted in two ways. In the first, all enzyme parameters are…”
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Characterization of protein-containing reversed micelles by Hilhorst, R, Verhaert, R M, Visser, A J

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Enzyme kinetics in reversed micelles by TYRAKOWSKA, Bozena, VERHAERT, Raymond M. D., HILHORST, Riet, VEEGER, Cees

“…The kinetic parameters of 20β‐hydroxysteroid dehydrogenase were determined in aqueous solutions and in reversed micellar media composed with either an anionic,…”
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Enzyme kinetics in reversed micelles. 3. Behaviour of 20 beta-hydroxysteroid dehydrogenase by Tyrakowska, B, Verhaert, R M, Hilhorst, R, Veeger, C

“…The kinetic parameters of 20 beta-hydroxysteroid dehydrogenase were determined in aqueous solutions and in reversed micellar media composed with either an…”
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Enzyme kinetics in reversed micelles by VERHAERT, Raymond M. D., TYRAKOWSKA, Bożena, HILHORST, Riet, SCHAAFSMA, Tjeerd J., VEEGER, Cees

“…Enoate reductase (EC 1.3.1.31) can stereospecificially reduce a variety of α,β‐unsaturated carboxylates. Its use was extended to apolar media by incorporating…”
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Enzyme kinetics in reversed micelles. 2. Behaviour of enoate reductase by Verhaert, R M, Tyrakowska, B, Hilhorst, R, Schaafsma, T J, Veeger, C

“…Enoate reductase (EC 1.3.1.31) can stereospecifically reduce a variety of alpha,beta-unsaturated carboxylates. Its use was extended to apolar media by…”
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