Search Results - "V. G. Bogush"
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Tissue Engineered Neural Constructs Composed of Neural Precursor Cells, Recombinant Spidroin and PRP for Neural Tissue Regeneration
Published in Scientific reports (28-02-2019)“…We have designed a novel two-component matrix (SPRPix) for the encapsulation of directly reprogrammed human neural precursor cells (drNPC). The matrix is…”
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Fermentation optimization of a Saccharomyces cerevisiae strain producing 1F9 recombinant spidroin
Published in Applied biochemistry and microbiology (01-12-2015)“…The fermentation of a Saccharomyces cerevisiae strain producing recombinant spidroin IF9 was optimized. A simplified two-stage scheme of the process was…”
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Effect of Shear on Structural Organization of Various Recombinant Spidroins
Published in Macromolecular symposia. (01-08-2022)“…To follow the structural reorganization of spidroin during its spinning, the rheological behavior of low‐concentration solution of recombinant spidroins rS1/9…”
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The Development of Bioadhesives Based on Recombinant Analogues of Spider Web Proteins
Published in Applied biochemistry and microbiology (01-12-2022)“…It has been shown that recombinant spidroins rS1/9 and rS2/12 that we previously developed exhibit adhesive properties with respect to both inorganic and…”
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Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells
Published in Doklady. Biological sciences (01-12-2023)“…The effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries…”
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Directed Neuronal Differentiation of SH-SY5Y Human Neuroblastoma Cells on 2D Matrices Containing Recombinant Spidroins Modified with Cell Adhesion Peptides
Published in Molecular genetics, microbiology and virology (01-09-2023)“…The use of efficient and inexpensive substrates (2D matrices) for cultivation and differentiation of nerve cells in vitro is important for the creation of…”
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Recombinant Spidroin Films Attenuate Individual Markers of Glucose Induced Aging in NIH 3T3 Fibroblasts
Published in Biochemistry (Moscow) (01-07-2020)“…The effect of bioresorbable materials on aging in cultured mouse NIH 3T3 fibroblasts treated with elevated glucose concentration was investigated. The cells…”
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formation of a quaternary structure by recombinant analogs of spider silk proteins
Published in Molecular biology (New York) (01-02-2010)“…The morphology of the fibers formed by recombinant analogs of dragline spider silk proteins, spidroins 1 and 2, was studied. It has been shown that the…”
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In vitro and in vivo biocompatibility studies of a recombinant analogue of spidroin 1 scaffolds
Published in Journal of biomedical materials research. Part A (01-01-2011)“…The goal of this study was to generate porous scaffolds from the genetically engineered protein, an analogue of Nephila clavipes spidroin 1 (rS1/9) and to…”
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Treatment of chronic liver disease using cell‑engineered constructs: morphofunctional characteristics
Published in Vestnik transplantologii i iskusstvennykh organov (01-12-2022)“…Objective: to study the effectiveness of correcting the morphofunctional characteristics of the liver in an experimental model of chronic liver disease (CLD),…”
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Photocurable Films Based on Fibroin and Gelatin for Skin Regeneration
Published in Moscow University biological sciences bulletin (2020)“…One of the current problems in regenerative medicine is the search for new approaches for skin restoration. A promising area is the use of biocompatible…”
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Photocurable Hydrogels Containing Spidroin or Fibroin
Published in Moscow University biological sciences bulletin (2018)“…Photocurable biocompatible hydrogels were fabricated from methacrylated gelatin and silk proteins (a recombinant analogue of spidroin from Nephila clavipes…”
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Novel 3D-microcarriers from recombinant spidroin for regenerative medicine
Published in Doklady. Biochemistry and biophysics (01-07-2015)“…Microcarriers generated from recombinant spidroin 1F9 are suitable for use as an injection material. The microcarriers were a heterogeneous mixture of microgel…”
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Recombinant 1F9 spidroin microgels for murine full-thickness wound repairing
Published in Doklady. Biochemistry and biophysics (2016)“…The study of the stimulating effect of the microgels (MGs) based on recombinant 1F9 spidroin on the regeneration of the deep skin wound in mice was carried…”
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Characterization of biodegradable cell micro and macro carriers based on recombinant spidroin
Published in Applied biochemistry and microbiology (01-12-2014)“…Gels, microgels, and matrices were prepared based on a previously developed recombinant spidroin, 1F9, produced by Saccharomyces cerevisiae yeast strain; their…”
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Use of buckwheat seed protease inhibitor gene for improvement of tobacco and potato plant resistance to biotic stress
Published in Biochemistry (Moscow) (01-03-2009)“…The possibility to use agrobacterial transformation of leaf discs to produce resistance to bacterial infections in tobacco and potato plants by introduction of…”
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COMPARATIVE ANALYSIS OF THREE-DIMENSIONAL NANOSTRUCTURE OF POROUS BIOCOMPATIBLE SCAFFOLDS MADE OF RECOMBINANT SPIDROIN AND SILK FIBROIN FOR REGENERATIVE MEDICINE
Published in Vestnik transplantologii i iskusstvennykh organov (26-05-2015)“…Aim. To perform a comparison of three-dimensional nanostructure of porous biocompatible scaffolds made of fibroin Bombix mori and recombinant spidroin rS1/9…”
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Construction of Synthetic Genes for Analogs of Spider Silk Spidroin 1 and Their Expression in Tobacco Plants
Published in Molecular biology (New York) (01-07-2003)“…To obtain transgenic tobacco plants expressing recombinant analogs of spider dragline silk spidroin 1, artificial 1f5 and 1f9 coding for spidroin 1 analogs…”
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Three-dimensional scaffold made from recombinant spider silk protein for tissue engineering
Published in Doklady. Biochemistry and biophysics (01-06-2009)“…The natural polymers (biopolymers) and their derivatives alginates, collagen, gelatin, chitosan, hyaluronic acid, and polyesters of bacterial origin are now…”
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Creation of a Producent, Optimization of Expression, and Purification of Recombinant Yersinia Pseudotuberculosis L-Asparaginase
Published in Bulletin of experimental biology and medicine (01-12-2011)“…Recombinant E. coli strain producing Y. pseudotuberculosis Q66CJ2 (YpA) L-asparaginase II was created. Gene ansB homologue encoding Y. pseudotuberculosis IP…”
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