Freeze-dried formulation for direct 99mTc-labeling ior-egf/r3 MAb: additives, biodistribution, and stability

Monoclonal antibodies (MAbs) have been useful for immunoscintigraphic applications in clinical diagnosis since they were introduced in nuclear medicine practice. The MAb ior egf/r3 developed at the Center of Molecular Immunology (Havana, Cuba) is a murine antibody that recognizes the human epidermal...

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Bibliographic Details
Published in:	Nuclear medicine and biology Vol. 26; no. 6; pp. 717 - 723
Main Authors:	Morales, Alejo A.Morales, Núñez-Gandolff, Gilda, Pérez, Niuvis Pérez, Véliz, Belkis Chico, Caballero-Torres, Idania, Ducongé, Jorge, Fernández, Eduardo, Crespo, Francisco Zayas, Veloso, Ana, Iznaga-Escobar, Normando
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-08-1999 Elsevier
Subjects:	Animals Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - pharmacokinetics Biodistribution Biological and medical sciences Drug Stability Female Freeze Drying Freeze-dried Glucose Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Miscellaneous. Technology Monoclonal antibodies Radiolabeling Radionuclide investigations Radiopharmaceuticals - chemistry Radiopharmaceuticals - pharmacokinetics Rats Rats, Wistar Technetium - pharmacokinetics Technetium-99m Tissue Distribution Monoclonal antibodies Radiolabeling Biodistribution Freeze-dried Technetium-99m Radionuclide study Freeze dried product Stability Rat Rodentia Technetium Monoclonal antibody Scintigraphy Vertebrata Mammalia Epidermal growth factor Efficiency Animal Preparation Distribution Medical imagery Pharmacokinetics Immune system
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Summary:	Monoclonal antibodies (MAbs) have been useful for immunoscintigraphic applications in clinical diagnosis since they were introduced in nuclear medicine practice. The MAb ior egf/r3 developed at the Center of Molecular Immunology (Havana, Cuba) is a murine antibody that recognizes the human epidermal growth factor receptor (EGF-R) and has been used widely in the radioimmunodiagnosis of tumors of epithelial origin. Based on the direct Schwarz method, the present report describes the preparation of a freeze-dried formulation for radiolabeling the MAb ior egf/r3 with 99mTc for immunoscintigraphic applications. Radiolabeling efficiency, effects on immunoreactivity, biodistribution, pharmacokinetic, and stability of the formulation are reported. The study demonstrated that the freeze-dried formulation can be labeled with 99mTc at high yield. The resulting 99mTc-labeled ior egf/r3 MAb can be used to visualize in vivo human tumors of epithelial origin by immunoscintigraphy studies. The kit does not need any other addition or purification at the time of tagging other than the requisite amount of pertechnetate (40–50 mCi). Because the contents of the kit are lyophilized, no special storage or transportation is required.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0969-8051 1872-9614
DOI:	10.1016/S0969-8051(99)00038-4