Search Results - "Uznański, B"
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The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets
Published in Acta biochimica polonica (1994)“…To examine whether the epidermal growth factor (EGF)-like domain Pro47-Asp87 is involved in the interaction of tissue plasminogen activator (t-PA) with…”
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2
Synthesis and expression in E. coli of the gene for human tumor necrosis factor (h alpha TNF)
Published in Archivum Immunologiae et Therapiae Experimentalis (1991)“…A gene coding for human tumor necrosis factor (h alpha TNF) has been assembled by ligating short oligodeoxyribonucleotides and cloning into plasmid vectors…”
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3
Expression of multivalent pre-S1 antigen of hepatitis B virus in Escherichia coli (synthetic oligodeoxynucleotides, surface antigens, recombinant DNA, fusion protein, beta-galactosidase)
Published in Archivum Immunologiae et Therapiae Experimentalis (1991)“…The nucleotide sequence encoding 30 amino acids (aa) of the pre-S1 envelope region of the human hepatitis B virus has been constructed from twenty chemically…”
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4
Expression of the Rz gene and the overlapping Rz1 reading frame present at the right end of the bacteriophage lambda genome
Published in Gene (15-07-1993)“…The Rz lysis gene of bacteriophage lambda was cloned into the expression vectors, pT7-3 and pT7-7. The recombinant plasmids expressed either a protein of an…”
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5
Antisense oligonucleotides: Stereocontrolled synthesis of phosphorothioate oligonucleotides
Published in Pure and applied chemistry (01-01-1993)Get full text
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6
Activity of acid deoxyribonuclease towards diastereoisomers of thymidyl 3'-(4-nitrophenyl phosphorothioate). Stereochemistry of transnucleotidylation reaction
Published in The Journal of biological chemistry (15-01-1986)“…The (Rp)- and (Sp)-diastereoisomers of thymidyl 3'-(4-nitrophenyl phosphorothioate) (1) were found to act as unusual substrates for acid deoxyribonuclease…”
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Diastereomers of thymidine 3'-O-(methanephosphonothioate): synthesis, absolute configuration and reaction with 3'-methoxyacetylthymidine under conditions of triester approach to oligonucleotide synthesis
Published in Acta biochimica polonica (1987)“…Diastereomers of the title compound were obtained and absolute configuration was assigned by means of stereochemical correlation. Their reaction with…”
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8
Synthesis of a gene encoding bovine substance P precursors and its expression in Escherichia coli
Published in Gene (15-08-1992)“…Using an efficient Escherichia coli expression system, we have been able to obtain the precursor of substance P, alpha-preprotachykinin (alpha PPT). The alpha…”
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9
Separation of [Sp]-3'-O-(5'-dimethoxytritylthymidyl)-5'-O-(3'camphanoyl thymidyl)-O-methyl phosphite
Published in Nucleic acids symposium series (1979) (1991)“…First isolation of diastereoisomerically enriched (d.p. 95%) [Sp]-3'-O-(5'-dimethoxytrityl tjymidyl)-5'-O-(3'-camphanoyl thymidyl)-O-methyl phosphite (1)…”
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10
A stereospecifically 18O-labelled deoxydinucleoside phosphate block for incorporation into an oligonucleotide
Published in Nucleic acids research (25-10-1983)“…Fully protected diastereoisomers of deoxyguanylyl (3' leads to 5') deoxyadenosine stereospecifically labelled on phosphorus with oxygen-18 have been…”
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11
Novel route to oligo(deoxyribonucleoside phosphorothioates). Stereocontrolled synthesis of P-chiral oligo(deoxyribonucleoside phosphorothiates)
Published in Nucleic acids research (11-11-1991)“…The synthesis and separation of diastereoisomerically pure 5'-O-DMT-nucleoside 3'-O-(2-thio-1,3,2-oxathiaphospholane) allows their use as synthons in…”
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12
Detection of tumor virus infections with HPV 16, 18, and 33 from cytologic material in the uterine neck using the PCR method
Published in Ginekologia polska (01-07-1993)“…A method for detection of oncogenic viruses HPV 16, 18, 33 based on enzymatic amplification (PCR) of specific viral DNA sequences is described. This technique…”
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13
Stereochemistry of base-catalyzed ring opening of 1,3,2-oxathiaphospholanes. Absolute configuration of 2-{N-[(Rc)-1-(.alpha.-naphthyl)ethyl]amino}-2-thiono-1,3,2-oxathiaphospholanes and O,S-dimethyl N-[(Rc)-1-(.alpha.-naphthyl)ethyl]phosphoramidothioates
Published in Journal of the American Chemical Society (01-12-1992)Get full text
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14
Duplex stabilities of phosphorothioate, methylphosphonate, and RNA analogs of two DNA 14-mers
Published in Nucleic acids research (11-06-1991)“…The duplex stabilities of various phosphorothioate, methylphosphonate, RNA and 2'-OCH3 RNA analogs of two self-complementary DNA 14-mers are compared…”
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15
Reversed-phase high-performance liquid chromatographic separation of diastereomeric phosphorothioate analogues of oligodeoxyribonucleotides and other backbone-modified congeners of DNA
Published in Journal of chromatography (19-06-1985)“…Diastereomeric phosphorothioate analogues of oligodeoxyribonucleotides, which were synthesized by an automated, solid-phase, phosphoramidite-coupling method,…”
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16
The isopropoxyacetic group for convenient base protection during solid-support synthesis of oligodeoxyribonucleotides and their triester analogs
Published in Nucleic acids research (26-06-1989)“…Isopropoxyacetic anhydride was successfully used for protection of exoaminofunctions of 2'-deoxyadenosine, -guanosine and -cytidine. N-isopropoxyacetylated…”
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17
The isopropoxyacetic group for convenient base protection during solid-support synthesis of oligodeoxy-ribonucleotides and their triester analogs
Published in Nucleic acids research (01-01-1989)“…Isopropoxyacetic anhydride was successfully used for protection of exoaminofunctions of 2'-deoxyadenosine, -guanosine and -cytidine. N-isopropoxyacetylated…”
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18
solid-phase synthesis of 2-5-linked oligoriboadenylates containing 8-bromoadenine
Published in Applied biochemistry and biotechnology (01-07-1997)“…To increase the accessibility of 8-bromo-2′,5′-oligoadenylates, we developed a synthesis of 2′-5′-linked oligoriboadenylates containing varying numbers of…”
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19
Stereochemistry of base-catalyzed ring opening of 1,3,2-oxathiaphospholanes. Absolute configuration of 2-{N-[(Rc)-1-(α-naphthyl)ethyl]amino}-2-thiono-1,3,2-oxathio-phospholanes and O,S-dimethyl N-[(Rc)-1-(α-naphthyl)ethyl]phosphoramidothiolates
Published in Journal of the American Chemical Society (1992)Get full text
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20
A stereospecifically super(18)O-labelled deoxydinucleoside phosphate block for incorporation into an oligonucleotide
Published in Nucleic acids research (01-01-1983)“…The DNA restriction endonucleases a group of more than 350 sequence specific nucleases, remain as yet unexplored with respect to their stereochemical…”
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