C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells

We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format th...

Full description

Saved in:

Bibliographic Details
Published in:	PloS one Vol. 9; no. 3; p. e90570
Main Authors:	Zevnik, Branko, Uyttersprot, Nathalie C, Perez, Ana V, Bothe, Gerald W M, Kern, Heidrun, Kauselmann, Gunther
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 05-03-2014 Public Library of Science (PLoS)
Subjects:	Agouti Signaling Protein - genetics Albinism Animals Base Sequence Biology Cells, Cultured Chimera - genetics Cloning Embryo Embryo Transfer Embryonic Stem Cells - physiology Embryos Female Genetic Loci Genomes Hair Color - genetics House mouse Laboratories Male Mice, Inbred BALB C Mice, Inbred C57BL Mice, Transgenic Monophenol Monooxygenase - genetics Mutation Offspring Retroelements Rodents Sperm Stem cells Superovulation Veterinary Science
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceived and designed the experiments: BZ NCU AVP GWMB GK. Performed the experiments: HK. Analyzed the data: BZ NCU AVP GWMB HK GK. Contributed reagents/materials/analysis tools: BZ NCU AVP GWMB HK GK. Wrote the paper: BZ AVP GK. Competing Interests: Taconic Farms Inc. is commercial sponsor of this research. N.U., A.P., G.B., H.K. and G.K. are affiliated as employees of Taconic Farms Inc., and B.Z. acts as consultant of Taconic Farms Inc. The authors declare that this does not alter their adherence to all the PLOS ONE policies on sharing data and materials.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0090570