Search Results - "Uyeda, Atsuko"
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Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system
Published in Journal of bioscience and bioengineering (01-02-2022)“…Attempts to create complex molecular systems that mimic parts of cellular systems using a bottom-up approach have become important in the field of biology…”
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Construction of an in Vitro Gene Screening System of the E. coli EmrE Transporter Using Liposome Display
Published in Analytical chemistry (Washington) (20-12-2016)“…Liposome display is a method that enables the directed evolution of membrane proteins in vitro. The method is based on the syntheses of membrane proteins using…”
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Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine‐5′‐Monophosphate
Published in Chembiochem : a European journal of chemical biology (02-12-2019)“…Polyphosphate kinase 2 (PPK2) transfer phosphate from inorganic polyphosphate to nucleotides. According to their activity, PPK2 enzymes are classified into…”
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An Immune-Stimulatory Helix–Loop–Helix Peptide: Selective Inhibition of CTLA-4–B7 Interaction
Published in ACS chemical biology (21-02-2020)“…Molecular-targeting peptides and mini-proteins are promising alternatives to antibodies in a wide range of applications in bioscience and medicine. We have…”
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Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation
Published in Chembiochem : a European journal of chemical biology (17-08-2015)“…Methanosarcina species pyrrolysyl‐tRNA synthetase (PylRS) attaches Pyl to its cognate amber suppressor tRNA. The introduction of two mutations (Y384F and…”
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Different protein localizations on the inner and outer leaflet of cell-sized liposomes using cell-free protein synthesis
Published in Synthetic biology (Oxford University Press) (01-01-2018)“…Abstract Membranes of living cells possess asymmetry. The inner and outer leaflets of the membrane consist of different phospholipid compositions, which are…”
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In vivo protein delivery to human liver-derived cells using hepatitis B virus envelope pre-S region
Published in Journal of bioscience and bioengineering (01-07-2008)“…Human hepatocyte-specific delivery of green fluorescent protein was succeeded in the mouse xenograft model by fusion with hepatitis B virus surface antigen…”
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Regulatory expression of MDP77 protein in the skeletal and cardiac muscles
Published in FEBS letters (09-10-2002)“…The mdp77 gene was first cloned from the cDNA library of denervated chick muscles, while its role(s) in vivo was unknown. In the present study, using specific…”
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Computational design of transmembrane pores
Published in Nature (London) (03-09-2020)“…Transmembrane channels and pores have key roles in fundamental biological processes 1 and in biotechnological applications such as DNA nanopore sequencing 2 –…”
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Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaA
Published in Journal of the American Chemical Society (04-12-2019)“…The bacterial cell surface structure has important roles for various cellular functions. However, research on reconstituting bacterial cell surface structures…”
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Generation of molecular-targeting helix-loop-helix peptides for inhibition of the interaction between cytotoxic T-lymphocyte-associated protein 4 and B7 in the dog
Published in Journal of Veterinary Medical Science (2022)“…Blocking the interaction between CD28 and B7 by cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is a potent immune checkpoint that prevents damage to host…”
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HSP90 Has Neurite-Promoting Activityin Vitrofor Telencephalic and Spinal Neurons of Chick Embryos
Published in Biochemical and biophysical research communications (01-12-1998)“…We purified a protein from the extract of denervated chick muscle. The protein had neurite-promoting activityin vitrofor the chick telencephalic neurons and…”
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Long-term effects of muscle-derived protein with molecular mass of 77 kDa (MDP77) on nerve regeneration
Published in Journal of neuroscience research (01-09-2005)“…The long‐term effects of the 77‐kDa muscle‐derived protein (MDP77) on motor and sensory nerve regeneration were examined in vivo. Fourteen‐millimeter bridge…”
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Neurocrescin is specifically cleaved after the sequence DESD in a caspase-3-independent manner
Published in Cellular and molecular neurobiology (01-12-2004)“…1. A neurite outgrowth factor, neurocrescin (NC), which we previously identified from an extract of denervated skeletal muscle, was endoproteolytically…”
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Muscle-specific protein MDP77 specifically promotes motor nerve regeneration in rats
Published in Neuroscience letters (29-04-2004)“…This study has examined the effects of recombinant human MDP77 (rhMDP77) on sciatic motor nerve regeneration in vivo. We carried out bridge grafting (14 mm)…”
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Identification of Active Regions for Neurite Outgrowth Activity of Neurocrescin
Published in Biochemical and biophysical research communications (02-03-2001)“…We previously identified and cloned a neurite outgrowth promoting protein, Neurocrescin (NC), from the extract of the chick denervated leg muscles. In this…”
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HSP90 has neurite-promoting activity in vitro for telencephalic and spinal neurons of chick embryos
Published in Biochemical and biophysical research communications (18-12-1998)“…We purified a protein from the extract of denervated chick muscle. The protein had neurite-promoting activity in vitro for the chick telencephalic neurons and…”
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714 Functional analysis of neurite-outgrowth factor, neurocrescin
Published in Neuroscience research (1997)Get full text
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MDP77: A Novel Neurite-Outgrowth-Promoting Protein Predominantly Expressed in Chick Muscles
Published in Biochemical and biophysical research communications (16-03-2000)“…A 4.7 kb chick cDNA clone that coded for the novel muscle-derived protein, MDP77, was isolated from a cDNA library of the denervated crus muscles using an…”
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Functional Analysis and Modeling of a Conformationally Constrained Arg-Gly-Asp Sequence Inserted into Human Lysozyme
Published in Biochemistry (Easton) (04-10-1994)“…To examine the effect of a conformational constraint introduced into the Arg-Gly-Asp (RGD) sequence on cell adhesion activity, we have constructed mutant…”
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