Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

In situ 3D bioprinting is a new emerging therapeutic modality for treating human skin diseases. The tissue spheroids have been previously suggested as a powerful tool in rapidly expanding bioprinting technology. It has been demonstrated that the regenerative potential of human dermal fibroblasts cou...

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Bibliographic Details
Published in:Cell and tissue research Vol. 390; no. 3; pp. 453 - 464
Main Authors: Koudan, Elizaveta V., Zorina, Alla I., Levin, Aleksandr A., Pereira, Frederico D. A. S., Petrov, Stanislav V., Karshieva, Saida Sh, Kasyanov, Vladimir A., Manturova, Natalya E., Ustyugov, Andrey Yu, Potekaev, Nikolay N., Parfenov, Vladislav A., Karalkin, Pavel A., Khesuani, Yusef D., Bulanova, Elena A., Kopnin, Pavel B., Isaev, Artur A., Mironov, Vladimir A., Zorin, Vadim L.
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-12-2022
Springer
Springer Nature B.V
Subjects:
Biomedical and Life Sciences
Biomedicine
Cell culture
Fibroblasts
Health aspects
Human Genetics
Mechanical properties
Molecular Medicine
Proteomics
Regular Article
Skin
Skin diseases
Spheroids
Dermal fibroblasts
Clonogenicity
Tissue spheroids
Regenerative potential
Proliferation
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Summary:In situ 3D bioprinting is a new emerging therapeutic modality for treating human skin diseases. The tissue spheroids have been previously suggested as a powerful tool in rapidly expanding bioprinting technology. It has been demonstrated that the regenerative potential of human dermal fibroblasts could be quantitatively evaluated in 2D cell culture and confirmed after implantation in vivo. However, the development of unbiassed quantitative criteria of the regenerative potential of 3D tissue spheroids in vitro before their in situ bioprinting remains to be investigated. Here it has been demonstrated for the first time that specific correlations exist between the regenerative potential of human dermal fibroblasts cultured in vitro as 2D cell monolayer with biological properties of 3D tissue spheroids fabricated from these fibroblasts. In vitro assessment of biological properties included diameter, spreading and fusion kinetics, and biomechanical properties of 3D tissue spheroids. This comprehensive characterization could be used to predict tissue spheroids’ regenerative potential in vivo.
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ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-022-03690-1