Poly(methacrylic Acid)-Induced Liposome Aggregation for Measuring Drug Entrapment

This report characterizes a procedure for rapidly and accurately determining the entrapment of vincristine or other water-soluble drugs in polyethylene glycol-derivatized liposomes. Rapid liposome aggregation with poly(methacrylic acid) and pelleting by mild centrifugation separates liposome-associa...

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Bibliographic Details
Published in:	Analytical biochemistry Vol. 229; no. 1; pp. 106 - 111
Main Authors:	Oto, E.K., Zalipsky, S., Quinn, Y.P., Zhu, G.Z., Uster, P.S.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 20-07-1995
Subjects:	Centrifugation Evaluation Studies as Topic Liposomes - chemistry Polyethylene Glycols Polymethacrylic Acids Reproducibility of Results Spectrophotometry, Ultraviolet Vincristine - analysis
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Summary:	This report characterizes a procedure for rapidly and accurately determining the entrapment of vincristine or other water-soluble drugs in polyethylene glycol-derivatized liposomes. Rapid liposome aggregation with poly(methacrylic acid) and pelleting by mild centrifugation separates liposome-associated vincristine from unentrapped drug. After collecting the supernatant fraction, the pellet is resuspended and solubilized in isopropyl alcohol. Quantitative uv spectrophotometry determines vincristine mass in both fractions. The mean accuracy (recovery) is 100.6% over an entrapped drug range from 70 to 99%. Within and between day precision of the method has a relative standard deviation of 0.76% for a single measurement.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-2697 1096-0309
DOI:	10.1006/abio.1995.1385