Search Results - "Urbauer, J L"

A Regulator That Inhibits Transcription by Targeting an Intersubunit Interaction of the RNA Polymerase Holoenzyme by Gregory, B. D., Nickels, B. E., Garrity, S. J., Severinova, E., Minakhin, L., R. J. Bieber Urbauer, Urbauer, J. L., Heyduk, T., Severinov, K., Hochschild, A., Ptashne, Mark

“…The structures of the bacterial RNA polymerase holoenzyme have provided detailed information about the intersubunit interactions within the holoenzyme…”
Get full text

Design and synthesis of multi-haem proteins by Robertson, Dan E, Farid, Ramy S, Moser, Christopher C, Urbauer, Jeffrey L, Mulholland, Stephen E, Pidikiti, Ravindernath, Lear, James D, Wand, A. Joshua, DeGrado, William F, Dutton, P. Leslie

“…A water-soluble, 62-residue, di-alpha-helical peptide has been synthesized which accommodates two bis-histidyl haem groups. The peptide assembles into a…”
Get full text

Calmodulin interacts with amphiphilic peptides composed of all D-amino acids by Fisher, Phyllis J, Prendergast, Franklyn G, Ehrhardt, Mark R, Urbauer, Jeffrey L, Wand, A. Joshua, Sedarous, Salah S, McCormick, Daniel J, Buckley, Paul J

“…Calmodulin binds to amphiphilic, helical peptides of a variety of amino-acid sequences. These peptides are usually positively charged, although there is…”
Get full text

Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule experiments by Allen, Michael W., Urbauer, Ramona J.Bieber, Zaidi, Asma, Williams, Todd D., Urbauer, Jeffrey L., Johnson, Carey K.

“…We present a method of labeling and immobilizing a low-molecular-weight protein, calmodulin (CaM), by fusion to a larger protein, maltose binding protein…”
Get full text

Metal dependence and thermodynamic characteristics of the beef heart mitochondrial adenosine triphosphatase by Dorgan, L J, Urbauer, J L, Schuster, S M

“…A systematic study was done examining the steady state kinetics of F1-catalyzed nucleotide hydrolysis in the presence of various activating divalent metal…”
Get full text

Metal-nucleotide structural characteristics during catalysis by beef heart mitochondrial F1 by Gruys, K J, Urbauer, J L, Schuster, S M

“…This study examined the nature of the metal-nucleotide complexes which serve as substrates, products, and intermediates in the beef heart mitochondrial ATPase…”
Get full text

Characterization of the Interactions between the Bacteriophage T4 AsiA Protein and RNA Polymerase by Simeonov, Mario F, Bieber Urbauer, Ramona J, Gilmore, Joshua M, Adelman, Karen, Brody, Edward N, Niedziela-Majka, Anita, Minakhin, Leonid, Heyduk, Tomasz, Urbauer, Jeffrey L

“…The anti-σ factor AsiA effects a change in promoter specificity of the Escherichia coli RNA polymerase via interactions with two conserved regions of the σ70…”
Get full text

Conserved regions 4.1 and 4.2 of sigma(70) constitute the recognition sites for the anti-sigma factor AsiA, and AsiA is a dimer free in solution by Urbauer, J L, Adelman, K, Urbauer, R J, Simeonov, M F, Gilmore, J M, Zolkiewski, M, Brody, E N

“…The association of the bacteriophage T4-encoded AsiA protein with the final sigma(70) subunit of the Escherichia coli RNA polymerase is one of the principal…”
Get full text

Solution Structure and Stability of the Anti-Sigma Factor AsiA: Implications for Novel Functions by Urbauer, Jeffrey L., Simeonov, Mario F., Ramona J. Bieber Urbauer, Adelman, Karen, Gilmore, Joshua M., Brody, Edward N.

“…Anti-sigma factors regulate prokaryotic gene expression through interactions with specific sigma factors. The bacteriophage T4 anti-sigma factor AsiA is a…”
Get full text

Structural Analysis of a Novel Interaction by Calmodulin: High-Affinity Binding of a Peptide in the Absence of Calcium by Urbauer, Jeffrey L, Short, James H, Dow, Linda K, Wand, A. Joshua

“…The interaction of apocalmodulin (apoCaM) with a peptide (Neurop) based on the primary sequence of the calmodulin-binding domain of neuromodulin has been…”
Get full text

Determination of the Chemical Mechanism of Malic Enzyme by Isotope Effects by Edens, William A, Urbauer, Jeffrey L, Cleland, W. W

“…Carbon-13 isotope effects have been determined for all four carbons of l-malate as a substrate for chicken liver malic enzyme, using either NADP or…”
Get full text

Energetics and dynamics of molecular recognition by calmodulin by Ehrhardt, Mark R, Urbauer, Jeffrey L, Wand, A. Joshua

“…Amide hydrogen exchange has been used to examine the structural dynamics and energetics of the interaction of a peptide corresponding to the calmodulin binding…”
Get full text

Determination of the Kinetic and Chemical Mechanism of Malic Enzyme Using (2R,3R)-erythro-Fluoromalate as a Slow Alternate Substrate by Urbauer, Jeffrey L, Bradshaw, Debra E, Cleland, W. W

“…(2R,3R)-erythro-Fluoromalate, but not the threo isomer, is a slow substrate for chicken liver malic enzyme with either NADP or 3-acetylpyridine-NADP (APADP) as…”
Get full text

Structural water in oxidized and reduced horse heart cytochrome c by Qi, Phoebe Xiurong, Urbauer, Jeffrey L, Fuentes, Ernesto J, Leopold, Mary Frances, Wand, A. Joshua

“…The existence of structural water in the interior of both oxidized and reduced horse-heart cytochrome c in solution is demonstrated using nuclear magnetic…”
Get full text

Resonance assignment strategies for the analysis of NMR spectra of proteins by LEOPOLD, M. F, URBAUER, J. L, WAND, A. J

“…Determination of the high resolution solution structure of a protein using nuclear magnetic resonance (NMR) spectroscopy requires that resonances observed in…”
Get full text

Synthesis of (2R,3R)-erythro- and (2R,3S)-threo-Fluoromalate Using Malic Dehydrogenase; Stereoselectivity of Malic Dehydrogenase by Urbauer, Jeffrey L, Bradshaw, Debra E, Cleland, W. W

“…3-Fluorooxalacetate is a substrate for malic dehydrogenase. When enzymatic reduction is slower than the rate of epimerization of the two enantiomers, only…”
Get full text

Main-chain NMR assignments for AsiA by Urbauer, J L, Adelman, K, Brody, E N

Get full text

Multiple isotope effects with alternative dinucleotide substrates as a probe of the malic enzyme reaction by Weiss, Paul M, Gavva, Sandhya R, Harris, Ben G, Urbauer, Jeffrey L, Cleland, W. W, Cook, Paul F

“…Deuterium isotope effects and 13C isotope effects with deuterium- and protium-labeled malate have been obtained for both NAD- and NADP-malic enzymes by using a…”
Get full text

Mechanistic studies of phosphoenolpyruvate carboxylase from Zea mays with (Z)- and (E)-3-fluorophosphoenolpyruvate as substrates by Janc, James W, Urbauer, Jeffrey L, O'Leary, Marion H, Cleland, W. W

“…The catalytic mechanism of phosphoenolpyruvate (PEP) carboxylase from Zea mays has been studied using (Z)- and (E)-3-fluorophosphoenolpyruvate (F-PEP) as…”
Get full text

Mechanism of Calmodulin Recognition of the Binding Domain of Isoform 1b of the Plasma Membrane Ca super(2+)-ATPase: Kinetic Pathway and Effects of Methionine Oxidation by Slaughter, B D, Urbauer, RJB, Urbauer, J L, Johnson, C K

“…Calmodulin (CaM) binds to a domain near the C-terminus of the plasma membrane Ca-ATPase (PMCA), causing the release of this domain and relief of its…”
Get full text