Effect of traditionally used herb Pedalium murex L. and its active compound pedalitin on urease expression – For the management of kidney stone

Effect of traditionally used herb Pedalium murex L. and its active compound pedalitin on urease expression – For the management of kidney stone

Pedalium murex L. is a medicinal herb that has been used for the treatment of diseases related to kidney in the traditional system of medicine. The current study aims to study the effect of ethyl acetate extract of P. murex (EAEP) and its fractionated compound pedalitin against urease production and...

Full description

Saved in:
Bibliographic Details
Published in:Saudi journal of biological sciences Vol. 27; no. 3; pp. 833 - 839
Main Authors: Ramadevi, S., Kaleeswaran, B., Ilavenil, S., Upgade, Akilesh, Tamilvendan, D., Rajakrishnan, R., Alfarhan, A.H., Kim, Y.-O., Kim, H.-J.
Format: Journal Article
Language:English
Published: Saudi Arabia Elsevier B.V 01-03-2020
Elsevier
Subjects:
Pedalitin
Pedalium murex
Proteus mirabilis
qPCR
UreC
Pedalium murex
Pedalitin
Proteus mirabilis
qPCR
UreC
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pedalium murex L. is a medicinal herb that has been used for the treatment of diseases related to kidney in the traditional system of medicine. The current study aims to study the effect of ethyl acetate extract of P. murex (EAEP) and its fractionated compound pedalitin against urease production and UreC gene expression in Proteus mirabilis. The selected reference strain Proteus mirabilis (MTCC 425) and the isolates culture of Proteus mirabilis were subjected to study the antibacterial efficacy of P. murex. Expression analysis of P. mirabilis urease gene was successfully done by QPCR. The ethyl acetate extract effectively inhibit the reference Proteus mirabilis and bacterial isolates of Proteus mirabilis in the clinical samples studied. EAEP has showed more potent activity (56.7%) against urease enzyme and pedalitin also exhibited potent activity (30.1%). Using qPCR, the expression of UreC gene of P. mirabilis was controlled by EAEP and also its bioactive compound pedalitin. The present study clearly demonstrated the potency of P. murex in controlling the growth of pathogenic P. mirabilis and to control the expression of urease enzyme production as well as to restrict the urease gene expression in P. mirabilis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1319-562X
2213-7106
DOI:10.1016/j.sjbs.2020.01.014