Search Results - "Uotila, Sanna T.H"

Introduction of histidine residues into avidin subunit interfaces allows pH-dependent regulation of quaternary structure and biotin binding by Nordlund, Henri R, Hytönen, Vesa P, Laitinen, Olli H, Uotila, Sanna T.H, Niskanen, Einari A, Savolainen, Janne, Porkka, Eevaleena, Kulomaa, Markku S

“…In order to turn the subunit association and biotin binding of avidin into pH-sensitive phenomena, we have replaced individually three amino acid residues in…”
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Construction of a Dual Chain Pseudotetrameric Chicken Avidin by Combining Two Circularly Permuted Avidins by Nordlund, Henri R, Laitinen, Olli H, Hytönen, Vesa P, Uotila, Sanna T H, Porkka, Eevaleena, Kulomaa, Markku S

“…Two distinct circularly permuted forms of chicken avidin were designed with the aim of constructing a fusion avidin containing two biotin-binding sites in one…”
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Rational Design of an Active Avidin Monomer by Laitinen, Olli H., Nordlund, Henri R., Hytönen, Vesa P., Uotila, Sanna T.H., Marttila, Ari T., Savolainen, Janne, Airenne, Kari J., Livnah, Oded, Bayer, Edward A., Wilchek, Meir, Kulomaa, Markku S.

“…Homotetrameric chicken avidin that binds four molecules of biotin was converted to a monomeric form (monoavidin) by mutations of two interface residues:…”
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Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces by Nordlund, Henri R, Laitinen, Olli H, Uotila, Sanna T H, Nyholm, Thomas, Hytönen, Vesa P, Slotte, J Peter, Kulomaa, Markku S

“…In this study we showed that tetrameric chicken avidin can be stabilized by introducing intermonomeric disulfide bridges between its subunits. These covalent…”
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Production of Hev b5 as a fluorescent biotin-binding tripartite fusion protein in insect cells by Nordlund, Henri R., Laitinen, Olli H., Uotila, Sanna T.H., Kulmala, Minna, Kalkkinen, Nisse, Kulomaa, Markku S.

“…The presented green fluorescent protein and streptavidin core-based tripartite fusion system provides a simple and efficient way for the production of proteins…”
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Enhancing the Thermal Stability of Avidin by Nordlund, Henri R., Laitinen, Olli H., Uotila, Sanna T.H., Nyholm, Thomas, Hytönen, Vesa P., Slotte, J. Peter, Kulomaa, Markku S.

“…In this study we showed that tetrameric chicken avidin can be stabilized by introducing intermonomeric disulfide bridges between its subunits. These covalent…”
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