Modeling of the controlled expression of a harmful protein by a three-plasmid harboring system

A genetically structured mathematical model was developed and used to evaluate the influence of molecular parameters involved in the expression of a harmful recombinant protein (SPA::EcoRI). The system consists of the controlled expression of the endonuclease EcoRI cloned in the plasmid pMTC48. The...

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Bibliographic Details
Published in:	Biotechnology and bioengineering Vol. 80; no. 5; pp. 544 - 551
Main Authors:	Unzaga, T. V., Díaz-Ricci, J. C., Rhee, J. I., Hernández, M. R., Schügerl, K.
Format:	Journal Article
Language:	English
Published:	New York Wiley Subscription Services, Inc., A Wiley Company 05-12-2002
Subjects:	Computer Simulation Deoxyribonuclease EcoRI - biosynthesis Deoxyribonuclease EcoRI - genetics DNA Damage DNA-Binding Proteins endonuclease Escherichia coli - classification Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Regulation, Bacterial - genetics Genetic Engineering - methods kinetic modeling Kinetics methylase Models, Biological Models, Chemical Models, Genetic Plasmids - genetics Protein Biosynthesis recombinant DNA Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Repressor Proteins - genetics Repressor Proteins - metabolism Reproducibility of Results Sensitivity and Specificity Species Specificity Staphylococcal Protein A - biosynthesis Staphylococcal Protein A - genetics Staphylococcus - genetics Transcription, Genetic Viral Proteins Viral Regulatory and Accessory Proteins
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Summary:	A genetically structured mathematical model was developed and used to evaluate the influence of molecular parameters involved in the expression of a harmful recombinant protein (SPA::EcoRI). The system consists of the controlled expression of the endonuclease EcoRI cloned in the plasmid pMTC48. The control is exerted by the lambda CI repressor expressed from the plasmid pRK248cIts. The deleterious effect of the activity of the enzyme EcoRI on the host DNA is prevented by the action of the EcoRI methylase that is expressed constitutively from a third plasmid, pEcoR4. The model includes molecular mechanisms involved in the regulation of the expression of these genes and is used to determine cultural conditions that maximize the production of the recombinant protein. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 544–551, 2002.
Bibliography:	ark:/67375/WNG-Q49D62MD-0 Alexander von Humboldt Foundation This article is dedicated to the memory of James E. Bailey. ArticleID:BIT10412 istex:95EA10FDD1DC123524B08BDB059B8D1C45928CD6 FundacióN Antorchas ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0006-3592 1097-0290
DOI:	10.1002/bit.10412