HSP70 as a marker of heat and humidity stress in Tarai buffalo

HSP70 as a marker of heat and humidity stress in Tarai buffalo

Heat and humidity stress is a constant challenge to buffalo rearing under tropical climatic conditions. Heat shock proteins (HSPs) constitute a ubiquitous class of highly conserved proteins that contribute to cell survival during different conditions of stress. The present study was carried out in T...

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Bibliographic Details
Published in:Tropical animal health and production Vol. 47; no. 1; pp. 111 - 116
Main Authors: Manjari, Rao, Yadav, Mrigakshi, Ramesh, Kandasamy, Uniyal, Sarveshwa, Rastogi, Sunil Kumar, Sejian, Veerasamy, Hyder, Iqbal
Format: Journal Article
Language:English
Published: Dordrecht Springer-Verlag 01-01-2015
Springer Netherlands
Springer Nature B.V
Subjects:
Animals
biomarkers
Biomarkers - metabolism
Biomedical and Life Sciences
buffaloes
Buffaloes - genetics
cell viability
Climatic conditions
climatic factors
correlation
Female
gene expression
heart rate
heat
heat stress
heat-shock protein 70
Heat-Shock Response
Hot Temperature
HSP70 Heat-Shock Proteins - blood
HSP70 Heat-Shock Proteins - genetics
Humidity
Lactation - physiology
Leukocytes, Mononuclear - metabolism
Life Sciences
mononuclear leukocytes
Physiology
quantitative polymerase chain reaction
Real-Time Polymerase Chain Reaction
rearing
Regular Articles
Respiratory Rate
Seasons
summer
temperature
Veterinary Medicine/Veterinary Science
winter
Zoology
Tarai buffalo
HSP70
Thermotolerance
PBMC
Humidity
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Summary:Heat and humidity stress is a constant challenge to buffalo rearing under tropical climatic conditions. Heat shock proteins (HSPs) constitute a ubiquitous class of highly conserved proteins that contribute to cell survival during different conditions of stress. The present study was carried out in Tarai buffaloes to study the expression of HSP70 in their peripheral blood mononuclear cells during different seasons and establish it as a marker of heat and humidity stress in buffaloes. Blood samples were collected from each healthy, non-lactating and non-pregnant buffalo above 2 years of age, once in the month of January (temperature-humidity index (THI) < 72) and in the month of May (THI > 72). Blood samples were also collected during October (THI = 72) to be used as calibrator/control. Real-time PCR was used to profile the HSP70 gene expression in the peripheral blood mononuclear cells (PBMCs). The relative expression values of HSP70 in Tarai buffalo was found to be significantly higher (P < 0.05) during summer season (2.37 ± 0.12) as compared to winter season (0.29 ± 0.04). The expression positively correlated with changes in physiological parameters like respiration rate (RR), pulse rate (PR) and rectal temperature (RT). In conclusion, it can be said that RR and HSP70 may act as characteristic physiological and cellular markers of heat and humidity stress in buffaloes.
Bibliography:http://dx.doi.org/10.1007/s11250-014-0692-4
ObjectType-Article-1
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ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-014-0692-4