Characterization of Ornithobacterium rhinotracheale from commercial layer chickens in eastern Japan

Abstract From a total of 72 commercial layer and pullet farms that were monitored in the eastern Japan area, 4 farms had mild to severe respiratory disease accompanied by decreased feed intake and drop in egg production. Microbiological analysis showed that 3 of the 4 farms, particularly from Fukush...

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Bibliographic Details
Published in:	Poultry science Vol. 97; no. 1; pp. 24 - 29
Main Authors:	Umali, D V, Shirota, K, Sasai, K, Katoh, H
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-01-2018
Subjects:	Animals Anti-Infective Agents - pharmacology Chickens Drug Resistance, Bacterial Female Flavobacteriaceae Infections - microbiology Flavobacteriaceae Infections - veterinary Japan Ornithobacterium - drug effects Ornithobacterium - physiology Poultry Diseases - microbiology layer chicken eastern Japan characterization Ornithobacterium rhinotracheale
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Summary:	Abstract From a total of 72 commercial layer and pullet farms that were monitored in the eastern Japan area, 4 farms had mild to severe respiratory disease accompanied by decreased feed intake and drop in egg production. Microbiological analysis showed that 3 of the 4 farms, particularly from Fukushima, Tochigi, and Ibaraki prefectures, were positive for Ornithobacterum rhinotracheale (ORT). Out of 65 birds examined, ORT was isolated in 21 birds (32.31%). All isolates were Gram-negative pleomorphic rods with a colony size of 0.05 mm, translucent with grayish coloration, and with butyric smell after 48 h of incubation in 10% chicken blood agar at 37°C under microaerophilic conditions. All isolates reacted positively in the p-nitrophenyl-β-d-galactopyranoside test within 3 h and were positive in cytochrome oxidase tests with an API 20NE identification system biocode of 0-0-2-0-0-0-4. An agar gel precipitation test showed that all isolates were serotype-A. All strains were positive in PCR by yielding a 784 bp amplicon of the 16S rRNA gene. All strains were resistant to amikacin, colistin, gentamicin, kanamycin, neomycin, polymyxin b, streptomycin, and sulfamethoxazole trimethoprim and susceptible to amoxicillin clavulanic acid, ampicillin, doxycycline, spectinomycin, and tetracycline. This study is the first characterization of ORT from commercial layer chickens in eastern Japan.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0032-5791 1525-3171
DOI:	10.3382/ps/pex254