Search Results - "Uhlenbeck, O"

Uniform Binding of Aminoacyl-tRNAs to Elongation Factor Tu by Thermodynamic Compensation by LaRiviere, Frederick J., Wolfson, Alexey D., Uhlenbeck, Olke C.

“…Elongation factor Tu (EF-Tu) binds all elongator aminoacyl-transfer RNAs (aa-tRNAs) for delivery to the ribosome during protein synthesis. Here, we show that…”
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tRNA residues that have coevolved with their anticodon to ensure uniform and accurate codon recognition by Olejniczak, Mikołaj, Uhlenbeck, Olke C.

“…The structure, phylogeny and in vivo function of the base pair formed between nucleotides 32 and 38 of the tRNA anticodon loop are reviewed. The A32–U38 pair,…”
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Keeping RNA happy by Uhlenbeck, O C

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Escherichia coli DbpA is an RNA helicase that requires hairpin 92 of 23S rRNA by Diges, Camille M., Uhlenbeck, Olke C.

“…Escherichia coli DbpA is a member of the DEAD/H family of proteins which has been shown to have robust ATPase activity only in the presence of a specific…”
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A re-investigation of the thio effect at the hammerhead cleavage site by Scott, Edmund C., Uhlenbeck, Olke C.

“…The effect of introducing a phosphorothioate at the hammerhead cleavage site was investigated using a kinetically well-characterized hammerhead. In buffers…”
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The Escherichia coli DEAD protein DbpA recognizes a small RNA hairpin in 23S rRNA by TSU, CHRISTOPHER A., KOSSEN, KARL, UHLENBECK, OLKE C.

“…The Escherichia coli DEAD protein DbpA is an RNA-specific ATPase that is activated by a 153-nt fragment within domain V of 23S rRNA. A series of RNA…”
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Is the sequence-specific binding of aminoacyl-tRNAs by EF-Tu universal among bacteria? by Schrader, Jared M., Uhlenbeck, Olke C.

“…Three base pairs in the T-stem are primarily responsible for the sequence-specific interaction of tRNA with Escherichia coli and Thermus thermophilus EF-Tu…”
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Amino acid specificity in translation by Dale, Taraka, Uhlenbeck, Olke C.

“…Recent structural and biochemical experiments indicate that bacterial elongation factor Tu and the ribosomal A-site show specificity for both the amino acid…”
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A small catalytic oligoribonucleotide by Uhlenbeck, Olke C

“…A 19-nucleotide RNA fragment can cause rapid, highly specific cleavage of a 24-nucleotide RNA fragment under physiological conditions. Because each 19-mer can…”
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Evidence for the role of solvated metal hydroxide in the hammerhead cleavage mechanism by Dahm, SueAnn C., Derrick, Wesley B., Uhlenbeck, Olke C.

“…The log of the rate of the chemical step of hammerhead cleavage in Mg2+ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates…”
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Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro by Sampson, J.R, Uhlenbeck, O.C

“…A recombinant plasmid was constructed with six synthetic DNA oligomers such that the DNA sequence corresponding to yeast tRNAPhe is flanked by a T7 promoter…”
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Role of divalent metal ions in the hammerhead RNA cleavage reaction by Dahm, SueAnn C, Uhlenbeck, Olke C

“…A hammerhead self-cleaving domain composed of two oligoribonucleotides was used to study the role of divalent metal ions in the cleavage reaction. Cleavage…”
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A Kinetic and Thermodynamic Framework for the Hammerhead Ribozyme Reaction by Hertel, Klemens J, Herschlag, Daniel, Uhlenbeck, Olke C

“…A hammerhead ribozyme (HH16) with eight potential base pairs in each of the substrate recognition helices stabilized product binding sufficiently to enable…”
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Synthesis of small RNAs using T7 RNA polymerase by Milligan, J F, Uhlenbeck, O C

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The hammerhead ribozyme by Blount, K F, Uhlenbeck, O C

“…The hammerhead ribozyme is a small RNA motif consisting of three helices that intersect at a conserved core. When correctly folded, the hammerhead ribozyme…”
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Numbering system for the hammerhead by HERTEL, K. J, PARDI, A, SYMONS, R. H, UHLENBECK, O. C, KOIZUMI, M, OHTSUKA, E, UESUGI, S, CEDERGREN, R, ECKSTEIN, F, GERLACH, W. L, HODGSON, R

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The Affinity of Elongation Factor Tu for an Aminoacyl-tRNA Is Modulated by the Esterified Amino Acid by Dale, Taraka, Sanderson, Lee E, Uhlenbeck, Olke C

“…When different mutations were introduced into the anticodon loop and at position 73 of YFA2, a derivative of yeast tRNAPhe, a single tRNA body was misacylated…”
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Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates by MILLIGAN, J. F, GROEBE, D. R, WITHERELL, G. W, UHLENBECK, O. C

“…A method is described to synthesize small RNAs of defined length and sequence using T7 RNA polymerase and templates of synthetic DNA which contain the T7…”
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Kinetics of intermolecular cleavage by hammerhead ribozymes by Fedor, Martha J, Uhlenbeck, Olke C

“…The hammerhead catalytic RNA effects cleavage of the phosphodiester backbone of RNA through a transesterification mechanism that generates products with…”
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Kinetic Characterization of Intramolecular and Intermolecular Hammerhead RNAs with Stem II Deletions by Long, David M., Uhlenbeck, Olke C.

“…A method is described to obtain intramolecular cleavage rates for the hammerhead ribozyme during in vitro transcription. By avoiding RNA purification and…”
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