Quantitative analysis of S. mutans and S. sobrinus cultivated independently and adhered to polished orthodontic composite resins

In orthodontics, fixed appliances placed in the oral cavity are colonized by microorganisms. The purpose of this study was to quantitatively determine the independent bacterial colonization of S. mutans and S. sobrinus in orthodontic composite resins. Seven orthodontic composite adhesives for bondin...

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Bibliographic Details
Published in:	Journal of applied oral science Vol. 20; no. 5; pp. 544 - 549
Main Authors:	Velazquez-Enriquez, Ulises, Scougall-Vilchis, Rogelio Jose, Contreras-Bulnes, Rosalia, Flores-Estrada, Jaime, Uematsu, Shinsuke, Yamaguchi, Ryozo
Format:	Journal Article
Language:	English
Published:	Brazil Faculdade de Odontologia de Bauru da Universidade de São Paulo 01-10-2012 Faculdade De Odontologia De Bauru - USP University of São Paulo
Subjects:	Acrylic Resins Analysis of Variance Bacterial Adhesion - physiology Bacterial Load Bisphenol A-Glycidyl Methacrylate Composite Resins Dental Cements Dental Polishing Dentistry DENTISTRY, ORAL SURGERY & MEDICINE Original Orthodontic Brackets - microbiology Phosphoric Acids Radioisotope Resin Cements Streptococcus mutans Streptococcus mutans - growth & development Streptococcus sobrinus Streptococcus sobrinus - growth & development Surface Properties White spot White spot Radioisotope Composite resins Streptococcus mutans Streptococcus sobrinus
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Summary:	In orthodontics, fixed appliances placed in the oral cavity are colonized by microorganisms. The purpose of this study was to quantitatively determine the independent bacterial colonization of S. mutans and S. sobrinus in orthodontic composite resins. Seven orthodontic composite adhesives for bonding brackets were selected and classified into 14 groups; (GIm, GIs) Enlight, (GIIm, GIIs) Grengloo, (GIIIm, GIIIs) Kurasper F, (GIVm, GIVs) BeautyOrtho Bond, (GVm, GVs) Transbond CC, (GVIm, GVIs) Turbo Bond II, (GVIIm, GVIIs) Blugloo. 60 blocks of 4x4x1 mm of each orthodontic composite resin were made (total 420 blocks), and gently polished with sand-paper and ultrasonically cleaned. S. mutans and S. sobrinus were independently cultivated. For the quantitative analysis, a radioactive marker was used to codify the bacteria (³H) adhered to the surface of the materials. The blocks were submerged in a solution with microorganisms previously radiolabeled and separated (210 blocks for S. mutans and 210 blocks for S. sobrinus) for 2 hours at 37 ºC. Next, the blocks were placed in a combustion system, to capture the residues and measure the radiation. The statistical analysis was calculated with the ANOVA test (Sheffè post-hoc). Significant differences of bacterial adhesion were found amongst the groups. In the GIm and GIs the significant lowest scores for both microorganisms were shown; in contrast, the values of GVII for both bacteria were significantly the highest. This study showed that the orthodontic composite resin evaluated in the GIm and GIs, obtained the lowest adherence of S. mutans and S. sobrinus, which may reduce the enamel demineralization and the risk of white spot lesion formation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1678-7757 1678-7765 1678-7765 1678-7757
DOI:	10.1590/S1678-77572012000500009