Search Results - "UCHIDA, Kazuhisa"

Stabilization of a single-stranded DNA of adeno-associated virus by inverted terminal repeats by Yuan, Yuzhe, Higashiyama, Kiyoko, Ito-Kudo, Emi, Masumi-Koizumi, Kyoko, Yusa, Keisuke, Uchida, Kazuhisa

“…Parvoviruses have evolved to possess a linear single-stranded DNA (ssDNA) genome ranging from 4 to 6.3 kb. Adeno-associated virus (AAV), a member of the…”
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Novel Approach for Obtaining Variable Domain of New Antigen Receptor with Different Physicochemical Properties from Japanese Topeshark ( Hemitriakis japanica ) by Nakada-Masuta, Tomofumi, Takeda, Hiroyuki, Uchida, Kazuhisa

“…Diverse candidate antibodies are needed to successfully identify therapeutic and diagnostic applications. The variable domain of IgNAR (VNAR), a shark…”
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A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies by Kono, Ken, Kataoka, Kiyoko, Yuan, Yuzhe, Yusa, Keisuke, Uchida, Kazuhisa, Sato, Yoji

“…Several xenogenic cell-based therapeutic products are currently under development around the world for the treatment of human diseases. Porcine islet cell…”
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The Absence of Fucose but Not the Presence of Galactose or Bisecting N-Acetylglucosamine of Human IgG1 Complex-type Oligosaccharides Shows the Critical Role of Enhancing Antibody-dependent Cellular Cytotoxicity by Shinkawa, Toyohide, Nakamura, Kazuyasu, Yamane, Naoko, Shoji-Hosaka, Emi, Kanda, Yutaka, Sakurada, Mikiko, Uchida, Kazuhisa, Anazawa, Hideharu, Satoh, Mitsuo, Yamasaki, Motoo, Hanai, Nobuo, Shitara, Kenya

“…An anti-human interleukin 5 receptor (hIL-5R) humanized immunoglobulin G1 (IgG1) and an anti-CD20 chimeric IgG1 produced by rat hybridoma YB2/0 cell lines…”
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Glycoform-dependent conformational alteration of the Fc region of human immunoglobulin G1 as revealed by NMR spectroscopy by Yamaguchi, Yoshiki, Nishimura, Mamiko, Nagano, Mayumi, Yagi, Hirokazu, Sasakawa, Hiroaki, Uchida, Kazuhisa, Shitara, Kenya, Kato, Koichi

“…The Fc portion of immunoglobulin G (IgG) expresses the biantennary complex type oligosaccharides at Asn297 of the C H2 domain of each heavy chain with…”
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Association between adeno‐associated virus genomic titers and intracellular plasmid levels by Masumi‐Koizumi, Kyoko, Yuan, Yuzhe, Higashiyama, Kiyoko, Yusa, Keisuke, Uchida, Kazuhisa

“…The recombinant adeno‐associated viral (rAAV) vector is one of the most effective viral vectors in gene therapy because of its low immunogenicity, high…”
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バイオ医薬品の製造・品質管理に関する人材育成の実践とAPEC CoEとしての国際貢献 by 内田, 和久

“…「はじめに」近年, 抗体医薬等の組換えタンパク質技術を応用したバイオ医薬品が多く開発され, これまで治療が難しかった疾患も克服できるようになってきた. 最近では, バイオ医薬品の主力である抗体医薬から, さらに開発の難易度が上がる低分子抗体や化学修飾した抗体, さらには遺伝子治療薬や細胞医薬へと領域が広がりつつある…”
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Engineering Chinese hamster ovary cells to maximize effector function of produced antibodies using FUT8 siRNA by Mori, Katsuhiro, Kuni-Kamochi, Reiko, Yamane-Ohnuki, Naoko, Wakitani, Masako, Yamano, Kazuya, Imai, Harue, Kanda, Yutaka, Niwa, Rinpei, Iida, Shigeru, Uchida, Kazuhisa, Shitara, Kenya, Satoh, Mitsuo

“…We explored the possibility of converting established antibody‐producing cells to cells producing high antibody‐dependent cellular cytotoxicity (ADCC)…”
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Practice of Human Resource Development Related to Biopharmaceutical Manufacturing and Quality Control and the International Contribution as Centers of Excellence of Asia-Pacific Economic Cooperation-Life Sciences Innovation Forum-Regulatory Harmonization Steering Committee by Uchida, Kazuhisa

“…In recent years, the development of biologics such as therapeutic antibodies has increased. Around 1980, Japan was the world's leading country in terms of…”
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Enhanced Fc-Dependent Cellular Cytotoxicity of Fc Fusion Proteins Derived from TNF Receptor II and LFA-3 by Fucose Removal from Asn-Linked Oligosaccharides by Shoji-Hosaka, Emi, Kobayashi, Yukari, Wakitani, Masako, Uchida, Kazuhisa, Niwa, Rinpei, Nakamura, Kazuyasu, Shitara, Kenya

“…Fucose removal from complex-type oligosaccharide of human IgGs results in a major enhancement of Fc-dependent cellular cytotoxicity. The aim of this study was…”
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Fucose Removal from Complex-Type Oligosaccharide Enhances the Antibody-Dependent Cellular Cytotoxicity of Single-Gene-Encoded Bispecific Antibody Comprising of Two Single-Chain Antibodies Linked to the Antibody Constant Region by Natsume, Akito, Wakitani, Masako, Yamane-Ohnuki, Naoko, Shoji-Hosaka, Emi, Niwa, Rinpei, Uchida, Kazuhisa, Satoh, Mitsuo, Shitara, Kenya

“…Bispecific antibodies (bsAbs) have the potential to extend binding selectivity, increase avidity and exert potent cytotoxicity due to the combination of dual…”
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A Chromatographic Approach for Elevating the Antibody-Dependent Cellular Cytotoxicity of Antibody Composites by Tojo, Setsuko, Okazaki, Akira, Wakitani, Masako, Shinkawa, Toyohide, Uchida, Kazuhisa, Suzawa, Toshiyuki

“…Recent studies have shown that antibodies with low fucose content in their oligosaccharides exhibit highly potent antibody-dependent cellular cytotoxicity…”
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Evaluation of next-generation sequencing performance for in vitro detection of viruses in biological products by Hirai, Takamasa, Kataoka, Kiyoko, Yuan, Yuzhe, Yusa, Keisuke, Sato, Yoji, Uchida, Kazuhisa, Kono, Ken

“…Next-Generation Sequencing (NGS) can detect nucleic acid sequences in a massively parallel sequencing. This technology is expected to be widely applied for the…”
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Applications and Limitations of Equilibrium Density Gradient Analytical Ultracentrifugation for the Quantitative Characterization of Adeno-Associated Virus Vectors by Hirohata, Kiichi, Yamaguchi, Yuki, Maruno, Takahiro, Shibuya, Risa, Torisu, Tetsuo, Onishi, Takayuki, Chono, Hideto, Mineno, Junichi, Yuzhe, Yuan, Higashiyama, Kiyoko, Masumi-Koizumi, Kyoko, Uchida, Kazuhisa, Yamamoto, Takenori, Uchida, Eriko, Okada, Takashi, Uchiyama, Susumu

“…Adeno-associated virus (AAV) vectors are produced as a mixture of the desired particle (full particle, FP), which is filled with the designed DNA,…”
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Quantitation of Residual Host Cell DNA in Recombinant Adeno-Associated Virus Using Droplet Digital Polymerase Chain Reaction by Higashiyama, Kiyoko, Yuan, Yuzhe, Hashiba, Noriko, Masumi-Koizumi, Kyoko, Yusa, Keisuke, Uchida, Kazuhisa

“…Recombinant adeno-associated virus (rAAV) is a viral vector commonly used in gene therapy. Residual host cell DNA is an impurity that has been associated with…”
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Infectivity assessment of porcine endogenous retrovirus using high-throughput sequencing technologies by Kono, Ken, Kataoka, Kiyoko, Yuan, Yuzhe, Yusa, Keisuke, Uchida, Kazuhisa, Sato, Yoji

“…Xenogenic cell-based therapeutic products are expected to alleviate the chronic shortage of human donor organs. For example, porcine islet cell products are…”
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Establishment of FUT8 knockout Chinese hamster ovary cells: An ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity by Yamane-Ohnuki, Naoko, Kinoshita, Satoko, Inoue-Urakubo, Miho, Kusunoki, Machi, Iida, Shigeru, Nakano, Ryosuke, Wakitani, Masako, Niwa, Rinpei, Sakurada, Mikiko, Uchida, Kazuhisa, Shitara, Kenya, Satoh, Mitsuo

“…To generate industrially applicable new host cell lines for antibody production with optimizing antibody‐dependent cellular cytotoxicity (ADCC) we disrupted…”
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Fucose Depletion from Human IgG1 Oligosaccharide Enhances Binding Enthalpy and Association Rate Between IgG1 and FcγRIIIa by Okazaki, Akira, Shoji-Hosaka, Emi, Nakamura, Kazuyasu, Wakitani, Masako, Uchida, Kazuhisa, Kakita, Shingo, Tsumoto, Kouhei, Kumagai, Izumi, Shitara, Kenya

“…Depletion of fucose from human IgG1 oligosaccharide improves its affinity for Fcγ receptor IIIa (FcγRIIIa). This is the first case where a glycoform…”
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Defucosylated chimeric anti-CC chemokine receptor 4 IgG1 with enhanced antibody-dependent cellular cytotoxicity shows potent therapeutic activity to T-cell leukemia and lymphoma by RINPEI NIWA, SHOJI-HOSAKA, Emi, SAKURADA, Mikiko, SHINKAWA, Toyohide, UCHIDA, Kazuhisa, NAKAMURA, Kazuyasu, MATSUSHIMA, Kouji, UEDA, Ryuzo, HANAI, Nobuo, SHITARA, Kenya

“…Human IgG1 antibodies with low fucose contents in their asparagine-linked oligosaccharides have been shown recently to exhibit potent antibody-dependent…”
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Preparation and Characterization of Alkoxide-Derived Lead-Free Piezoelectric Barium Zirconate Titanate Thin Films with Different Compositions by Ohno, Tomoya, Uchida, Kazuhisa, Sakamoto, Naonori, Fu, Desheng, Wakiya, Naoki, Matsuda, Takeshi, Suzuki, Hisao

“…Lead-free barium zirconate titanate Ba(Zr 1-x Ti x )O 3 ; BZT100$x$ $0\leq x\leq 0.1$) thin films were deposited on lanthanum nickel oxide (LNO)/Pt/SiO 2 /Si…”
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