Search Results - "UBEIRA, Florencio M"
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Field evaluation of the enhanced MM3-COPRO ELISA test for the diagnosis of Fasciola hepatica infection in sheep
Published in PloS one (24-03-2022)“…Fasciolosis is a severe zoonosis responsible for major economic losses in livestock. The enhanced MM3-COPRO test (eMM3-COPRO) and the commercial version BIO K…”
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Increased specificity of Fasciola hepatica excretory-secretory antigens combining negative selection on hydroxyapatite and salt precipitation
Published in Scientific reports (16-02-2024)“…A single and rapid method to obtain an antigenic fraction of excretory-secretory antigens (ESAs) from Fasciola hepatica suitable for serodiagnosis of…”
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Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens
Published in Scientific reports (08-03-2022)“…The family Anisakidae, mainly represented by Anisakis simplex s.l. and Pseudoterranova decipiens , encompasses zoonotic nematodes infecting many marine fish…”
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Review of MARCH-INSIDE & complex networks prediction of drugs: ADMET, anti-parasite activity, metabolizing enzymes and cardiotoxicity proteome biomarkers
Published in Current drug metabolism (01-05-2010)“…In this communication we carry out an in-depth review of a very versatile QSPR-like method. The method name is MARCH-INSIDE (MARkov CHains Ivariants for…”
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Rapid Enhanced MM3-COPRO ELISA for Detection of Fasciola Coproantigens
Published in PLoS neglected tropical diseases (20-07-2016)“…ELISA-based methods of detecting Fasciola cathepsins in feces are powerful techniques for diagnosing infections by F. hepatica and F. gigantica. In the last…”
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Predicting drugs and proteins in parasite infections with topological indices of complex networks: theoretical backgrounds, applications, and legal issues
Published in Current pharmaceutical design (01-01-2010)“…Quantitative Structure-Activity Relationship (QSAR) models have been used in Pharmaceutical design and Medicinal Chemistry for the discovery of anti-parasite…”
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Fasciola spp: Mapping of the MF6 epitope and antigenic analysis of the MF6p/HDM family of heme-binding proteins
Published in PloS one (21-11-2017)“…MF6p/FhHDM-1 is a small cationic heme-binding protein which is recognized by the monoclonal antibody (mAb) MF6, and abundantly present in parenchymal cells and…”
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Review of QSAR models for enzyme classes of drug targets: Theoretical background and applications in parasites, hosts, and other organisms
Published in Current pharmaceutical design (01-01-2010)“…The number of protein 3D structures without function annotation in Protein Data Bank (PDB) has been steadily increased. Many of these proteins are relevant for…”
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Anisakis Sensitization in the Croatian fish processing workers: Behavioral instead of occupational risk factors?
Published in PLoS neglected tropical diseases (01-01-2020)“…We undertook the first study systematically evaluating the risk of Anisakis-sensitization in Croatian fish-processing workers and potential genetic…”
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Complex Network Study of the Immune Epitope Database for Parasitic Organisms
Published in Current topics in medicinal chemistry (09-02-2018)“…Complex network approach allows the representation and analysis of complex systems of interacting agents in an ordered and effective manner, thus increasing…”
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Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides
Published in PloS one (01-06-2016)“…Lipopolysaccharide (LPS), the major constituent of the outer membrane of Gram-negative bacteria, can trigger severe inflammatory responses during bacterial…”
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Anti-Anisakis IgE seroprevalence in the healthy Croatian coastal population and associated risk factors
Published in PLoS neglected tropical diseases (01-02-2014)“…The main objective of the study was to determine the degree of sensitization to Anisakis spp. antigens in healthy coastal population of Dalmatia given the high…”
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In-plate recapturing of a dual-tagged recombinant Fasciola antigen (FhLAP) by a monoclonal antibody (US9) prevents non-specific binding in ELISA
Published in PloS one (01-02-2019)“…Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged…”
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Temporal genetic variation of Fasciola hepatica from sheep in Galicia (NW Spain)
Published in Veterinary parasitology (30-04-2015)“…•We tested for possible changes in the genetic diversity and structure of Fasciola hepatica from sheep over time.•We found low genetic differentiation between…”
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Model for Vaccine Design by Prediction of B-Epitopes of IEDB Given Perturbations in Peptide Sequence, In Vivo Process, Experimental Techniques, and Source or Host Organisms
Published in Journal of immunology research (01-01-2014)“…Perturbation methods add variation terms to a known experimental solution of one problem to approach a solution for a related problem without known exact…”
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Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis
Published in PLoS neglected tropical diseases (01-11-2011)“…Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be…”
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Influence of life history traits on the population genetic structure of parasitic helminths: a minireview
Published in Folia parasitologica (06-11-2015)“…Parasite life history traits influence the rate of gene flow between populations and the effective population size, both of which determine the levels of…”
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Different Fish-Eating Habits and Cytokine Production in Chronic Urticaria with and without Sensitization against the Fish-Parasite Anisakis simplex
Published in Allergology international (01-06-2013)“…Anisakis simplex sensitization has been associated with acute, but also with chronic urticaria. The objective of this study is to characterize chronic…”
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Synthetic oxoisoaporphine alkaloids: in vitro, in vivo and in silico assessment of antileishmanial activities
Published in PloS one (2013)“…Leishmaniasis is a growing health problem worldwide. As there are certain drawbacks with the drugs currently used to treat human leishmaniasis and resistance…”
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ANISERP: a new serpin from the parasite Anisakis simplex
Published in Parasites & vectors (28-07-2015)“…Serine proteinase inhibitors (serpins) finely regulate serine proteinase activity via a suicide substrate-like inhibitory mechanism. In parasitic nematodes,…”
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