Ribosome-messenger recognition in the absence of the Shine-Dalgarno interactions

In an attempt to understand how Escherichia coli ribosomes recognize the initiator codon on mRNAs lacking the Shine-Dalgamo (SD) sequence, we have studied 30S initiation complex formation in extension inhibition (toeprinting) experiments using (-SD)mRNAs which are known to be reliably translated in...

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Bibliographic Details
Published in:	FEBS letters Vol. 337; no. 2; pp. 189 - 194
Main Authors:	Tzareva, N.V., Makhno, V.I., Boni, I.V.
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 10-01-1994
Subjects:	A1MV RNA 4, the subgenomic mRNA for alfalfa mosaic virus coat protein alfalfa mosaic virus Alfalfa mosaic virus - genetics AMV RTase, reverse transcriptase from avian myoblastosis virus Base Sequence Codon - metabolism DNA Primers Escherichia coli Escherichia coli - metabolism Footprinting Glucuronidase - biosynthesis GUS, the sequence coding for β-glucuronidase of Escherichia coli Molecular Sequence Data Omega (Ω), 5'-untranslated sequence of tobacco mosaic virus (TMV) RNA Plant viral RNA leader Protein Biosynthesis Protein S1 Ribosomal Proteins - metabolism Ribosomes - metabolism RNA, Messenger - biosynthesis RNA, Messenger - metabolism RNA, Viral - metabolism RNA-protein interaction RT, reverse transcription tobacco mosaic virus Tobacco Mosaic Virus - genetics Toeprinting Translational initiation Footprinting Omega (Ω), 5'-untranslated sequence of tobacco mosaic virus (TMV) RNA Translational initiation Toeprinting A1MV RNA 4, the subgenomic mRNA for alfalfa mosaic virus coat protein Plant viral RNA leader RT, reverse transcription AMV RTase, reverse transcriptase from avian myoblastosis virus RNA-protein interaction GUS, the sequence coding for β-glucuronidase of Escherichia coli Protein S1
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Summary:	In an attempt to understand how Escherichia coli ribosomes recognize the initiator codon on mRNAs lacking the Shine-Dalgamo (SD) sequence, we have studied 30S initiation complex formation in extension inhibition (toeprinting) experiments using (-SD)mRNAs which are known to be reliably translated in E. coli: the plant viral messenger A1MV RNA 4 and two chimaeric mRNAs coding for β-glucuronidase (GUS) and bearing the 5'-untranslated sequence of TMV RNA Ω or the Ω-derived sequence (CAA) n as 5'-leaders. Ribosomal protein Sl and IF3 have been found to be indispensable for translational initiation. Protein S1 appears to be a key recognition element. S1 binds to sequences within the leaders of (-SD)mRNAs thus providing their affinity to E. coli ribosomes.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(94)80271-8