Search Results - "Tymiak, A. A."

Approach To Evaluating Dried Blood Spot Sample Stability during Drying Process and Discovery of a Treated Card To Maintain Analyte Stability by Rapid On-Card pH Modification by Liu, Guowen, Ji, Qin C, Jemal, Mohammed, Tymiak, Adrienne A, Arnold, Mark E

“…Unstable drug candidates often lead to complexity for both sample collection and bioanalysis. Dried blood spot (DBS) technology is believed to be a viable…”
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Enantioselective chromatography in drug discovery by Zhang, Yingru, Wu, Dauh-Rurng, Wang-Iverson, David B., Tymiak, Adrienne A.

“…The majority of the drugs approved today as well as the drug candidates entering the development process are single enantiomers. Dramatic advances in chiral…”
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Characterization of disulfide linkages in recombinant human granulocyte-colony stimulating factor by Mo, Jingjie, Tymiak, Adrienne A., Chen, Guodong

“…RATIONALE Recombinant human G granulocyte‐colony stimulating factor (rhG‐CSF) produced in Escherichia coli is a non‐glycosylated polypeptide containing five…”
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A Tris (2-Carboxyethyl) Phosphine (TCEP) Related Cleavage on Cysteine-Containing Proteins by Liu, Peiran, O'Mara, Brian W., Warrack, Bethanne M., Wu, Wei, Huang, Yunping, Zhang, Yihong, Zhao, Rulin, Lin, Mei, Ackerman, Michael S., Hocknell, Peter K., Chen, Guodong, Tao, Li, Rieble, Siegfried, Wang, Jack, Wang-Iverson, David B., Tymiak, Adrienne A., Grace, Michael J., Russell, Reb J.

“…Introduced in the late 1980s as a reducing reagent, Tris (2-carboxyethyl) phosphine (TCEP) has now become one of the most widely used protein reductants. To…”
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Optimization and Simulation of Tandem Column Supercritical Fluid Chromatography Separations Using Column Back Pressure as a Unique Parameter by Wang, Chunlei, Tymiak, Adrienne A, Zhang, Yingru

“…Tandem column supercritical fluid chromatography (SFC) has demonstrated to be a useful technique to resolve complex mixtures by serially coupling two columns…”
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Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications by Wei, Hui, Mo, Jingjie, Tao, Li, Russell, Reb J., Tymiak, Adrienne A., Chen, Guodong, Iacob, Roxana E., Engen, John R.

“…•Hydrogen/deuterium exchange mass spectrometry (HDX-MS) technology for assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS…”
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Physicochemical Characterization of a Ouabain Isomer Isolated from Bovine Hypothalamus by Tymiak, Adrienne A., Norman, Jon A., Bolgar, Mark, DiDonato, Gerald C., Lee, Helen, Parker, William L., Lo, Lee-Chiang, Berova, Nina, Nakanishi, Koji, Haber, Edgar, Haupert, Garner T.

“…Recent reports have shown the presence of a ouabain-like inhibitor of Na+/K+-ATPase in humans. We have purified a bovine hypothalamic Na+/K+-ATPase inhibitory…”
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Development and implementation of a stereoselective normal-phase liquid chromatography–tandem mass spectrometry method for the determination of intrinsic metabolic clearance in human liver microsomes by Zhang, Yingru, Caporuscio, Christian, Dai, Jun, Witkus, Michael, Rose, Anne, Santella, Joseph, D’Arienzo, Celia, Wang-Iverson, David B., Tymiak, Adrienne A.

“…The stereoselective determination of stereoisomers in biological samples provides vital information on stereospecific metabolism and pharmacokinetic profiles…”
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Fast Photochemical Oxidation of Proteins (FPOP) Maps the Epitope of EGFR Binding to Adnectin by Yan, Yuetian, Chen, Guodong, Wei, Hui, Huang, Richard Y.-C., Mo, Jingjie, Rempel, Don L., Tymiak, Adrienne A., Gross, Michael L.

“…Epitope mapping is an important tool for the development of monoclonal antibodies, mAbs, as therapeutic drugs. Recently, a class of therapeutic mAb…”
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Orthogonal Mass Spectrometry-Based Footprinting for Epitope Mapping and Structural Characterization: The IL‑6 Receptor upon Binding of Protein Therapeutics by Li, Ke Sherry, Chen, Guodong, Mo, Jingjie, Huang, Richard Y.-C, Deyanova, Ekaterina G, Beno, Brett R, O’Neil, Steve R, Tymiak, Adrienne A, Gross, Michael L

“…Higher-order structure (HOS) is a crucial determinant for the biological functions and quality attributes of protein therapeutics. Mass spectrometry (MS)-based…”
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Mapping the Energetic Epitope of an Antibody/Interleukin-23 Interaction with Hydrogen/Deuterium Exchange, Fast Photochemical Oxidation of Proteins Mass Spectrometry, and Alanine Shave Mutagenesis by Li, Jing, Wei, Hui, Krystek, Stanley R, Bond, Derek, Brender, Ty M, Cohen, Daniel, Feiner, Jena, Hamacher, Nels, Harshman, Johanna, Huang, Richard Y.-C, Julien, Susan H, Lin, Zheng, Moore, Kristina, Mueller, Luciano, Noriega, Claire, Sejwal, Preeti, Sheppard, Paul, Stevens, Brenda, Chen, Guodong, Tymiak, Adrienne A, Gross, Michael L, Schneeweis, Lumelle A

“…Epitope mapping the specific residues of an antibody/antigen interaction can be used to support mechanistic interpretation, antibody optimization, and epitope…”
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The role of chromatographic and chiroptical spectroscopic techniques and methodologies in support of drug discovery for atropisomeric drug inhibitors of Bruton’s tyrosine kinase by Dai, Jun, Wang, Chunlei, Traeger, Sarah C., Discenza, Lorell, Obermeier, Mary T., Tymiak, Adrienne A., Zhang, Yingru

“…•An array of systematic analytical methods meets the challenges of atropisomerism.•Chromatography and chiroptical spectroscopy play key roles to study…”
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Probing Conformational Dynamics of Tau Protein by Hydrogen/Deuterium Exchange Mass Spectrometry by Huang, Richard Y.-C., Iacob, Roxana E., Sankaranarayanan, Sethu, Yang, Ling, Ahlijanian, Michael, Tao, Li, Tymiak, Adrienne A., Chen, Guodong

“…Fibrillization of the microtubule-associated protein tau has been recognized as one of the signature pathologies of the nervous system in Alzheimer’s disease,…”
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Characterization and quantification of histidine degradation in therapeutic protein formulations by size exclusion-hydrophilic interaction two dimensional-liquid chromatography with stable-isotope labeling mass spectrometry by Wang, Chunlei, Chen, Sike, Brailsford, John A., Yamniuk, Aaron P., Tymiak, Adrienne A., Zhang, Yingru

“…•An SEC-HILIC 2DLC method to characterize histidine degradation in protein formulations.•First usage of stable-isotope dilution MS for heart-cutting 2DLC…”
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Structural mass spectrometry in biologics discovery: advances and future trends by Mo, Jingjie, Tymiak, Adrienne A., Chen, Guodong

“…► We present the workflow for MS-based structural characterization of biologics in biopharmaceutical drug discovery. ► We describe ETD-MS and HDX-MS techniques…”
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Na,K-ATPase Inhibitors from Bovine Hypothalamus and Human Plasma Are Different from Ouabain: Nanogram Scale CD Structural Analysis by Zhao, Ning, Lo, Lee-Chiang, Berova, Nina, Nakanishi, Koji, Tymiak, Adrienne A, Ludens, James H, Haupert, Garner T

“…The specific, high affinity binding of plant-derived digitalis glycosides by the mammalian sodium and potassium transporting adenosine triphosphatase…”
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High-resolution MS for structural characterization of protein therapeutics: advances and future directions by Wei, Hui, Tymiak, Adrienne A, Chen, Guodong

“…High-resolution MS (HRMS) is a central analytical technique for the study of biomolecules and is widely used in the biopharmaceutical industry. This paper…”
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Utility of Ion Mobility Mass Spectrometry for Drug-to-Antibody Ratio Measurements in Antibody-Drug Conjugates by Huang, Richard Y.-C., Deyanova, Ekaterina G., Passmore, David, Rangan, Vangipuram, Deshpande, Shrikant, Tymiak, Adrienne A., Chen, Guodong

“…Antibody-drug conjugates (ADCs) are emerging modalities in the pharmaceutical industry. Characterization of ADC’s drug-to-antibody ratio (DAR) becomes a key…”
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Characterization of Aggregation Propensity of a Human Fc-Fusion Protein Therapeutic by Hydrogen/Deuterium Exchange Mass Spectrometry by Huang, Richard Y.-C., Iacob, Roxana E., Krystek, Stanley R., Jin, Mi, Wei, Hui, Tao, Li, Das, Tapan K., Tymiak, Adrienne A., Engen, John R., Chen, Guodong

“…Aggregation of protein therapeutics has long been a concern across different stages of manufacturing processes in the biopharmaceutical industry. It is often…”
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An automated high throughput liquid chromatography-mass spectrometry process to assess the metabolic stability of drug candidates by Drexler, Dieter M, Belcastro, James V, Dickinson, Kenneth E, Edinger, Kurt J, Hnatyshyn, Serhiy Y, Josephs, Jonathan L, Langish, Robert A, McNaney, Colleen A, Santone, Kenneth S, Shipkova, Petia A, Tymiak, Adrienne A, Zvyaga, Tatyana A, Sanders, Mark

“…An automated high throughput process, termed the MetFast assay, is described to assess in vitro the general microsomal cytochrome P450 beta-nicotinamide…”
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