Evaluation of ribotyping as epidemiologic tool for typing Escherichia coli serogroup O157 isolates

Evaluation of ribotyping as epidemiologic tool for typing Escherichia coli serogroup O157 isolates

A total of 121 representative Escherichia coli O157:H7 and O157:NM (nonmotile) isolates were characterized by ribotype, phage type, verotoxin genotype, and genomic fingerprints generated by pulsed-field gel electrophoresis. Ribotyping was not able to discriminate between O157:H7 isolates, and phage...

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Bibliographic Details
Published in:Journal of Clinical Microbiology Vol. 34; no. 3; pp. 720 - 723
Main Authors: Martin, I.E, Tyler, S.D, Tyler, K.D, Khakharia, R, Johnson, W.M
Format: Journal Article
Language:English
Published: Washington, DC American Society for Microbiology 01-03-1996
Subjects:
Bacterial Typing Techniques
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Electrophoresis, Gel, Pulsed-Field
Escherichia coli
Escherichia coli O157 - classification
food contamination
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Serotyping
Typing
Investigation method
Ribosomal RNA
Escherichia coli
Molecular epidemiology
Bacteria
Genotype
Clinical isolate
Enterobacteriaceae
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Summary:A total of 121 representative Escherichia coli O157:H7 and O157:NM (nonmotile) isolates were characterized by ribotype, phage type, verotoxin genotype, and genomic fingerprints generated by pulsed-field gel electrophoresis. Ribotyping was not able to discriminate between O157:H7 isolates, and phage typing and pulsed-field gel electrophoresis were the most valuable and discriminatory techniques.
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ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.34.3.720-723.1996