Search Results - "Tycko, Josh"

Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes by Hess, Gaelen T., Tycko, Josh, Yao, David, Bassik, Michael C.

“…The past several years have seen an explosion in development of applications for the CRISPR-Cas9 system, from efficient genome editing, to high-throughput…”
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Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity by Tycko, Josh, Myer, Vic E., Hsu, Patrick D.

“…Advances in the development of delivery, repair, and specificity strategies for the CRISPR-Cas9 genome engineering toolbox are helping researchers understand…”
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Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements by Mumbach, Maxwell R, Satpathy, Ansuman T, Boyle, Evan A, Dai, Chao, Gowen, Benjamin G, Cho, Seung Woo, Nguyen, Michelle L, Rubin, Adam J, Granja, Jeffrey M, Kazane, Katelynn R, Wei, Yuning, Nguyen, Trieu, Greenside, Peyton G, Corces, M Ryan, Tycko, Josh, Simeonov, Dimitre R, Suliman, Nabeela, Li, Rui, Xu, Jin, Flynn, Ryan A, Kundaje, Anshul, Khavari, Paul A, Marson, Alexander, Corn, Jacob E, Quertermous, Thomas, Greenleaf, William J, Chang, Howard Y

“…High-resolution contact maps of active enhancers and target genes generated by H3K27ac HiChIP in primary human cells provide rational guides to link noncoding…”
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Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements by Tycko, Josh, Wainberg, Michael, Marinov, Georgi K., Ursu, Oana, Hess, Gaelen T., Ego, Braeden K., Aradhana, Li, Amy, Truong, Alisa, Trevino, Alexandro E., Spees, Kaitlyn, Yao, David, Kaplow, Irene M., Greenside, Peyton G., Morgens, David W., Phanstiel, Douglas H., Snyder, Michael P., Bintu, Lacramioara, Greenleaf, William J., Kundaje, Anshul, Bassik, Michael C.

“…Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these…”
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CasKAS: direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping by Marinov, Georgi K, Kim, Samuel H, Bagdatli, S Tansu, Higashino, Soon Il, Trevino, Alexandro E, Tycko, Josh, Wu, Tong, Bintu, Lacramioara, Bassik, Michael C, He, Chuan, Kundaje, Anshul, Greenleaf, William J

“…Detecting and mitigating off-target activity is critical to the practical application of CRISPR-mediated genome and epigenome editing. While numerous methods…”
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Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells by Tycko, Josh, Barrera, Luis A., Huston, Nicholas C., Friedland, Ari E., Wu, Xuebing, Gootenberg, Jonathan S., Abudayyeh, Omar O., Myer, Vic E., Wilson, Christopher J., Hsu, Patrick D.

“…Therapeutic genome editing with Staphylococcus aureus Cas9 (SaCas9) requires a rigorous understanding of its potential off-target activity in the human genome…”
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Modeling the efficacy of CRISPR gene drive for snail immunity on schistosomiasis control by Grewelle, Richard E, Perez-Saez, Javier, Tycko, Josh, Namigai, Erica K. O, Rickards, Chloe G, De Leo, Giulio A

“…CRISPR gene drives could revolutionize the control of infectious diseases by accelerating the spread of engineered traits that limit parasite transmission in…”
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Gene drives for schistosomiasis transmission control by Maier, Theresa, Wheeler, Nicolas James, Namigai, Erica K O, Tycko, Josh, Grewelle, Richard Ernest, Woldeamanuel, Yimtubezinash, Klohe, Katharina, Perez-Saez, Javier, Sokolow, Susanne H, De Leo, Giulio A, Yoshino, Timothy P, Zamanian, Mostafa, Reinhard-Rupp, Jutta

“…Schistosomiasis is one of the most important and widespread neglected tropical diseases (NTD), with over 200 million people infected in more than 70 countries;…”
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Lessons learned from the clinical development and market authorization of Glybera by Bryant, Laura M, Christopher, Devin M, Giles, April R, Hinderer, Christian, Rodriguez, Jesse L, Smith, Jenessa B, Traxler, Elizabeth A, Tycko, Josh, Wojno, Adam P, Wilson, James M

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Publisher Correction: Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells by Tycko, Josh, Barrera, Luis A., Huston, Nicholas C., Friedland, Ari E., Wu, Xuebing, Gootenberg, Jonathan S., Abudayyeh, Omar O., Myer, Vic E., Wilson, Christopher J., Hsu, Patrick D.

“…The original HTML version of this Article incorrectly listed an affiliation of Josh Tycko as ‘Department of Genetics, Stanford University School of Medicine,…”
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Large-scale mapping and mutagenesis of human transcriptional effector domains by DelRosso, Nicole, Tycko, Josh, Suzuki, Peter, Andrews, Cecelia, Aradhana, Mukund, Adi, Liongson, Ivan, Ludwig, Connor, Spees, Kaitlyn, Fordyce, Polly, Bassik, Michael C., Bintu, Lacramioara

“…Human gene expression is regulated by more than 2,000 transcription factors and chromatin regulators 1 , 2 . Effector domains within these proteins can…”
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Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome by Durrant, Matthew G., Fanton, Alison, Tycko, Josh, Hinks, Michaela, Chandrasekaran, Sita S., Perry, Nicholas T., Schaepe, Julia, Du, Peter P., Lotfy, Peter, Bassik, Michael C., Bintu, Lacramioara, Bhatt, Ami S., Hsu, Patrick D.

“…Large serine recombinases (LSRs) are DNA integrases that facilitate the site-specific integration of mobile genetic elements into bacterial genomes. Only a few…”
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High-Throughput Discovery and Characterization of Human Transcriptional Effectors by Tycko, Josh, DelRosso, Nicole, Hess, Gaelen T., Aradhana, Banerjee, Abhimanyu, Mukund, Aditya, Van, Mike V., Ego, Braeden K., Yao, David, Spees, Kaitlyn, Suzuki, Peter, Marinov, Georgi K., Kundaje, Anshul, Bassik, Michael C., Bintu, Lacramioara

“…Thousands of proteins localize to the nucleus; however, it remains unclear which contain transcriptional effectors. Here, we develop HT-recruit, a pooled assay…”
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A genome-wide genetic screen uncovers determinants of human pigmentation by Bajpai, Vivek K, Swigut, Tomek, Mohammed, Jaaved, Naqvi, Sahin, Arreola, Martin, Tycko, Josh, Kim, Tayne C, Pritchard, Jonathan K, Bassik, Michael C, Wysocka, Joanna

“…Skin color, one of the most diverse human traits, is determined by the quantity, type, and distribution of melanin. In this study, we leveraged the…”
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Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways by Tycko, Josh, Adam, Virginie S, Crosariol, Marco, Ohlstein, Jason, Sanmiguel, Julio, Tretiakova, Anna P, Roy, Soumitra, Worgall, Stefan, Wilson, James M, Limberis, Maria P

“…Infants and older adults are especially vulnerable to infection by respiratory syncytial virus (RSV), which can cause significant illness and irreparable…”
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Development of compact transcriptional effectors using high-throughput measurements in diverse contexts by Tycko, Josh, Van, Mike V., Aradhana, DelRosso, Nicole, Ye, Hanrong, Yao, David, Valbuena, Raeline, Vaughan-Jackson, Alun, Xu, Xiaoshu, Ludwig, Connor, Spees, Kaitlyn, Liu, Katherine, Gu, Mingxin, Khare, Venya, Mukund, Adi Xiyal, Suzuki, Peter H., Arana, Sophia, Zhang, Catherine, Du, Peter P., Ornstein, Thea S., Hess, Gaelen T., Kamber, Roarke A., Qi, Lei S., Khalil, Ahmad S., Bintu, Lacramioara, Bassik, Michael C.

“…Transcriptional effectors are protein domains known to activate or repress gene expression; however, a systematic understanding of which effector domains…”
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Multicenter integrated analysis of noncoding CRISPRi screens by Yao, David, Tycko, Josh, Oh, Jin Woo, Bounds, Lexi R., Gosai, Sager J., Lataniotis, Lazaros, Mackay-Smith, Ava, Doughty, Benjamin R., Gabdank, Idan, Schmidt, Henri, Guerrero-Altamirano, Tania, Siklenka, Keith, Guo, Katherine, White, Alexander D., Youngworth, Ingrid, Andreeva, Kalina, Ren, Xingjie, Barrera, Alejandro, Luo, Yunhai, Yardımcı, Galip Gürkan, Tewhey, Ryan, Kundaje, Anshul, Greenleaf, William J., Sabeti, Pardis C., Leslie, Christina, Pritykin, Yuri, Moore, Jill E., Beer, Michael A., Gersbach, Charles A., Reddy, Timothy E., Shen, Yin, Engreitz, Jesse M., Bassik, Michael C., Reilly, Steven K.

“…The ENCODE Consortium’s efforts to annotate noncoding cis -regulatory elements (CREs) have advanced our understanding of gene regulatory landscapes. Pooled,…”
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The sound of silence: Transgene silencing in mammalian cell engineering by Cabrera, Alan, Edelstein, Hailey I, Glykofrydis, Fokion, Love, Kasey S, Palacios, Sebastian, Tycko, Josh, Zhang, Meng, Lensch, Sarah, Shields, Cara E, Livingston, Mark, Weiss, Ron, Zhao, Huimin, Haynes, Karmella A, Morsut, Leonardo, Chen, Yvonne Y, Khalil, Ahmad S, Wong, Wilson W, Collins, James J, Rosser, Susan J, Polizzi, Karen, Elowitz, Michael B, Fussenegger, Martin, Hilton, Isaac B, Leonard, Joshua N, Bintu, Lacramioara, Galloway, Kate E, Deans, Tara L

“…To elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims to build and integrate synthetic…”
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High-throughput functional characterization of combinations of transcriptional activators and repressors by Mukund, Adi X, Tycko, Josh, Allen, Sage J, Robinson, Stephanie A, Andrews, Cecelia, Sinha, Joydeb, Ludwig, Connor H, Spees, Kaitlyn, Bassik, Michael C, Bintu, Lacramioara

“…Despite growing knowledge of the functions of individual human transcriptional effector domains, much less is understood about how multiple effector domains…”
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High-throughput discovery and characterization of viral transcriptional effectors in human cells by Ludwig, Connor H, Thurm, Abby R, Morgens, David W, Yang, Kevin J, Tycko, Josh, Bassik, Michael C, Glaunsinger, Britt A, Bintu, Lacramioara

“…Viruses encode transcriptional regulatory proteins critical for controlling viral and host gene expression. Given their multifunctional nature and high…”
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