Search Results - "Tunitskaya, V.L."

Recent studies of T7 RNA polymerase mechanism by Kochetkov, S.N, Rusakova, E.E, Tunitskaya, V.L

“…Bacteriophage T7 RNA polymerase (T7 RNAP) is known to be one of the simplest enzymes catalyzing RNA synthesis. In contrast to most RNA polymerases known, this…”
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Synthesis of mixed ribo/deoxyribopolynucleotides by mutant T7 RNA polymerase by Gudima, S.O, Kostyuk, D.A, Grishchenko, O.I, Tunitskaya, V.L, Memelova, L.V, Kochetkov, S.N

“…Synthesis of deoxynucleotide-containing RNA-like single-stranded polynucleotides (dcRNAs) using the Y639F, S641A mutant of T7 RNA polymerase (T7 RNAP) was…”
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Mutant T7 RNA polymerase is capable of catalyzing DNA primer extension reaction by Rusakova, E.E, Tunitskaya, V.L, Memelova, L.V, Kochetkova, S.V, Kostyuk, D.A, Kochetkov, S.N

“…The mutant T7 RNA polymerase (T7 RNAP), containing two substitutions (Y639F, S641A) was earlier shown to utilize both rNTP and dNTP in a transcription-like…”
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Biogenic polyamines spermine and spermidine activate RNA polymerase and inhibit RNA helicase of hepatitis C virus by Korovina, A. N., Tunitskaya, V. L., Khomutov, M. A., Simonian, A. R., Khomutov, A. R., Ivanov, A. V., Kochetkov, S. N.

“…Influence of the biogenic polyamines spermine, spermidine, and putrescine as well as their derivatives on the replication enzymes of hepatitis C virus (HCV)…”
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Inhibition of DNA gyrase by levofloxacin and related fluorine-containing heterocyclic compounds by Tunitskaya, V L, Khomutov, A R, Kochetkov, S N, Kotovskaya, S K, Charushin, V N

“…Fluoroquinolones are an important class of modern and efficient antibacterial drugs with a broad spectrum of activity. Levofloxacin (the optically active form…”
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Hepatitis C virus RNA-dependent RNA polymerase: study on the inhibition mechanism by pyrogallol derivatives by Kozlov, M V, Polyakov, K M, Ivanov, A V, Filippova, S E, Kuzyakin, A O, Tunitskaya, V L, Kochetkov, S N

“…Pyrogallol reversibly and noncompetitively inhibits the activity of the hepatitis C RNA-dependent RNA polymerase. Based on molecular modeling of the inhibitor…”
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Base-Modified Ribonucleosides as Potential Anti-Hepatitis C virus Agents by Ivanov, A.V., Smirnova, O.A., Golubeva, N.A., Ivanov, M.A., Tunitskaya, V.L., Shipitsyn, A.V., Alexandrova, L.A.

“…Abstract Four novel series of base modified ribonucleoside analogues were synthesized and evaluated as potential anti-HCV agents. For two compounds notable…”
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Interaction of HIV-1 reverse transcriptase with modified oligonucleotide primers containing 2'-O-beta-D-ribofuranosyladenosine by Golubeva, A S, Ermolinsky, B S, Efimtseva, E V, Tunitskaya, V L, van Aerschot, A, Herdewijn, P, Mikhailov, S N, Kochetkov, S N

“…Modified synthetic oligodeoxyribonucleotides containing 2'-O-beta-D-ribofuranosyladenosine were used as primers in the RNA-dependent DNA synthesis catalyzed by…”
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Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA by Kostyuk, D.A., Dragan, S.M., Lyakhov, D.L., Rechinsky, V.O., Tunitskaya, V.L., Chernov, B.K., Kochetkov, S.N.

“…A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but is…”
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Substrate properties of C′-methyl UTP derivatives in T7 RNA polymerase reactions. Evidence for N-type NTP conformation by Tunitskaya, V.L, Rusakova, E.E, Padyukova, N.Sh, Ermolinsky, B.S, Chernyi, A.A, Kochetkov, S.N, Lysov, Yu.P, Mikhailov, S.N

“…The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7 RNA polymerase (T7…”
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On the functional role of the Tyr-639 residue of bacteriophage T7 RNA polymerase by Rechinsky, V.O., Kostyuk, D.A., Tunitskaya, V.L., Kochetkov, S.N.

“…Substitution or Asp for a Tyr residue normally present at position 639 of the bacteriophage T7 RNA polymerase leads to a drastic drop in the enzymatic…”
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Tyr-571 is involved in the T7 RNA polymerase binding to its promoter by Rechinsky, V.O., Tunitskaya, V.L., Dragan, S.M., Kostyuk, D.A., Kochetkov, S.N.

“…The in vitro studies of three T7 RNA polymerase point mutants suggest that substitutions of Ala and Thr for Pro-563 and of Ser for Tyr-571 have little effect…”
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Targeted mutagenesis identifies Asp-569 as a catalytically critical residue in T7 RNA polymerase by Rechinsky, V O, Chernov, B K, Dragan, S M, Kostyuk, D A, Tunitskaya, V L, Kochetkov, S N

“…In order to look more closely at a well-conserved region in T7 RNA polymerase (T7 RNAP) containing, as shown earlier, the functionally essential residues…”
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The mechanisms of the cyclic AMP-dependent regulation of the enzymes of the 2′,5′-oligoadenylate system by Itkes, A.V., Turpaev, K.T., Kartasheva, O.N., Tunitskaya, V.L., Kafiani, C.A.

“…The cAMP-dependent induction of 2,5-oligoadenylate (2-5A) synthetase and cAMP-dependent inhibition of 2-5A phosphodiesterase are shown. Variations in…”
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Activities of cAMP-dependent protein kinase and enzymes of 2',5'-oligoadenylate metabolism in NIH 3T3 cells deepening into the resting state by Itkes, A V, Kartasheva, O N, Tunitskaya, V L, Turpaev, K T, Kafiani, C A, Severin, E S

“…The activity of cAMP-dependent protein kinase was found to increase continuously in the NIH 3T3 cells, deepening into the resting state. The increase…”
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Adenosine-5′( p-fluorosulfonylphenylphosphate) as a tool for investigation of cooperative properties of Na,K-ATPase by Emelyanov, S.A., Tunitskaya, V.L., Boldyrev, A.A.

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Regulation of 2′,5′-oligo(A) synthetase activity in theophylline-treated NIH 3T3 cells by Itkes, A.V., Turpaev, K.T., Kartasheva, O.N., Tunitskaya, V.L., Kafiani, C.A., Severin, E.S.

“…Sequential treatment of NIH 3T3 cells with theophylline and actinomycin D results in an enhancement of 2′,5′-oligo(A) synthetase activity 2–3-fold exceeding…”
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Investigation of the adenosine 3', 5'-cyclic phosphate binding site of pig brain histone kinase with the aid of some analogues of adenosine 3', 5'-cyclic phosphate by Severin, E S, Nesterova, M V, Sashchenko, L P, Rasumova, V V

“…2'-O-Chloroacetyl cyclic AMP, 2'-O-acrylyl cyclic AMP and N-6, 2'-O-diacrylyl cyclic AMP were synthesized by the reaction of cyclic AMP with chloroacetic and…”
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