Search Results - "Tunitskaya, V. L."

Inhibition of DNA gyrase by levofloxacin and related fluorine-containing heterocyclic compounds by Tunitskaya, V L, Khomutov, A R, Kochetkov, S N, Kotovskaya, S K, Charushin, V N

“…Fluoroquinolones are an important class of modern and efficient antibacterial drugs with a broad spectrum of activity. Levofloxacin (the optically active form…”
Get full text

Synthesis and biological properties of pyrimidine 4′-fluoronucleosides and 4′-fluorouridine 5′-O-triphosphate by Ivanov, M. A, Ludva, G. S, Mukovnya, A. V, Kochetkov, S. N, Tunitskaya, V. L, Alexandrova, L. A

“…4′-Fluoro-2′,3′-O-isopropylidenecytidine was synthesized by the treatment of 5′-O-acetyl-4′-fluoro-2′,3′-O-isopropylideneuridine with triazole and…”
Get full text

Structural-functional analysis of bacteriophage T7 RNA polymerase by Tunitskaya, V L, Kochetkov, S N

“…This review summarizes our results of the structural and functional studies of bacteriophage T7 DNA-dependent RNA polymerase (T7 RNAP). Particular features of…”
Get full text

Biogenic polyamines spermine and spermidine activate RNA polymerase and inhibit RNA helicase of hepatitis C virus by Korovina, A. N., Tunitskaya, V. L., Khomutov, M. A., Simonian, A. R., Khomutov, A. R., Ivanov, A. V., Kochetkov, S. N.

“…Influence of the biogenic polyamines spermine, spermidine, and putrescine as well as their derivatives on the replication enzymes of hepatitis C virus (HCV)…”
Get full text

Analysis of the Domains of Hepatitis C Virus Core and NS5A Proteins that Activate the Nrf2/ARE Cascade by Smirnova, O A, Ivanova, O N, Mukhtarov, F Sh, Tunitskaya, V L, Jansons, J, Isaguliants, M G, Kochetkov, S N, Ivanov, A V

“…The hepatitis C virus (HCV) triggers a chronic disease that is often accompanied by a spectrum of liver pathologies and metabolic alterations. The oxidative…”
Get full text

Recent studies of T7 RNA polymerase mechanism by Kochetkov, S.N, Rusakova, E.E, Tunitskaya, V.L

“…Bacteriophage T7 RNA polymerase (T7 RNAP) is known to be one of the simplest enzymes catalyzing RNA synthesis. In contrast to most RNA polymerases known, this…”
Get full text

New non-nucleoside inhibitors of hepatitis C virus RNA-dependent RNA polymerase by Ivanov, A V, Kozlov, M V, Kuzyakin, A O, Kostyuk, D A, Tunitskaya, V L, Kochetkov, S N

“…Recombinant RNA-dependent RNA polymerase of hepatitis C virus was purified using a bacterial expression system (Escherichia coli). The system for enzyme…”
Get full text

Enhancement of the immune response by codelivery of hepatitis C virus recombinant DNA and proteins of the replicative complex by Masalova, O. V., Lesnova, E. I., Permyakova, K. Yu, Ivanov, A. V., Tunitskaya, V. L., Kushch, A. A.

“…The plasmids encoding amino acid sequences corresponding to the hepatitis C virus (HCV) are regarded as promising candidates for anti-HCV vaccines. However,…”
Get full text

Interaction of HIV-1 reverse transcriptase with modified oligonucleotide primers containing 2'-O-beta-D-ribofuranosyladenosine by Golubeva, A S, Ermolinsky, B S, Efimtseva, E V, Tunitskaya, V L, van Aerschot, A, Herdewijn, P, Mikhailov, S N, Kochetkov, S N

“…Modified synthetic oligodeoxyribonucleotides containing 2'-O-beta-D-ribofuranosyladenosine were used as primers in the RNA-dependent DNA synthesis catalyzed by…”
Get full text

Synthesis of mixed ribo/deoxyribopolynucleotides by mutant T7 RNA polymerase by Gudima, S.O, Kostyuk, D.A, Grishchenko, O.I, Tunitskaya, V.L, Memelova, L.V, Kochetkov, S.N

“…Synthesis of deoxynucleotide-containing RNA-like single-stranded polynucleotides (dcRNAs) using the Y639F, S641A mutant of T7 RNA polymerase (T7 RNAP) was…”
Get full text

The combined application of nucleotide and amino acid sequences of NS3 hepatitis C virus protein, DNA encoding granulocyte macrophage colony-stimulating factor, and inhibitor of regulatory T cells induces effective immune responce against Hepatitis C virus by Masalova, O. V., Lesnova, E. I., Shingarova, L. N., Tunitskaya, V. L., Ulanova, T. I., Burkov, A. N., Kushch, A. A.

“…Hepatitis C is related to the most important socially significant human infectious diseases. However, there is no vaccine for the hepatitis C virus. The…”
Get full text

Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA by Kostyuk, D.A., Dragan, S.M., Lyakhov, D.L., Rechinsky, V.O., Tunitskaya, V.L., Chernov, B.K., Kochetkov, S.N.

“…A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but is…”
Get full text

Hepatitis C virus helicase/NTPase: an efficient expression system and new inhibitors by Mukovnya, A. V., Tunitskaya, V. L., Khandazhinskaya, A. L., Golubeva, N. A., Zakirova, N. F., Ivanov, A. V., Kukhanova, M. K., Kochetkov, S. N.

“…A method has been developed for obtaining a full-length protein NS3 of hepatitis C virus with the yield of 6.5 mg/liter of cell culture, and conditions for…”
Get full text

Photoaffinity Modification of Bacteriophage T7 DNA-Dependent RNA Polymerase with a Reaction Product Containing an Azido Derivative of UTP by Tunitskaya, V. L., Memelova, L. V., Skoblov, Yu. S., Kochetkov, S. N.

“…The possibility of using 5-[3-(E)-(4-azido-2,3,5,6-tetrafluorobenzamido)-propenyl-1]-UTP (N^sub 3^-TFBP-UTP) as a photoaffinity modifier of T7 DNA-dependent…”
Get full text

Mutant T7 RNA polymerase is capable of catalyzing DNA primer extension reaction by Rusakova, E.E, Tunitskaya, V.L, Memelova, L.V, Kochetkova, S.V, Kostyuk, D.A, Kochetkov, S.N

“…The mutant T7 RNA polymerase (T7 RNAP), containing two substitutions (Y639F, S641A) was earlier shown to utilize both rNTP and dNTP in a transcription-like…”
Get full text

On the functional role of the Tyr-639 residue of bacteriophage T7 RNA polymerase by Rechinsky, V.O., Kostyuk, D.A., Tunitskaya, V.L., Kochetkov, S.N.

“…Substitution or Asp for a Tyr residue normally present at position 639 of the bacteriophage T7 RNA polymerase leads to a drastic drop in the enzymatic…”
Get full text

Interactions between the hepatitis C virus protein NS3 and polymethylene derivatives of nucleic bases by Mukovnya, A. V., Komissarov, V. V., Kritsyn, A. M., Mitkevich, V. A., Tunitskaya, V. L., Kochetkov, S. N.

“…Nonstructural protein 3 (NS3) of hepatitis C virus plays a key role in the functioning of the virus. NS3 displays three enzymatic activities, namely, protease…”
Get full text

Point Contacts of T7 RNA Polymerase in the Promoter Complex, as Determined with Phosphate-Activated Oligonucleotide Derivatives by Filippova, S E, Ivanovskaya, M G, Romanova, E A, Tunitskaya, V L, Kochetkov, S N

“…The contacts between phosphate groups of promoter DNA and Lys or His of T7 RNA polymerase (Pol) in the Pol-promoter complex were studied with single- and…”
Get full text

New furano- and pyrrolo[2,3-d]pyrimidine nucleosides and their 5′-O-triphosphates: Synthesis and biological properties by Ivanov, M. A., Ivanov, A. V., Krasnitskaya, I. A., Smirnova, O. A., Karpenko, I. L., Belanov, E. F., Prasolov, V. S., Tunitskaya, V. L., Alexandrova, L. A.

“…Bicyclic furano[2,3- d ]pyrimidine ribonucleosides were synthesized by Pd(0)-and CuI-catalyzed coupling of 5-iodouridine with terminal alkynes. The treatment…”
Get full text

Hepatitis C virus RNA-dependent RNA polymerase: study on the inhibition mechanism by pyrogallol derivatives by Kozlov, M V, Polyakov, K M, Ivanov, A V, Filippova, S E, Kuzyakin, A O, Tunitskaya, V L, Kochetkov, S N

“…Pyrogallol reversibly and noncompetitively inhibits the activity of the hepatitis C RNA-dependent RNA polymerase. Based on molecular modeling of the inhibitor…”
Get full text