Search Results - "Tungsuchat‐Huang, Tarinee"
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Posttranscriptional tuning of gene expression over a large dynamic range in synthetic tobacco chloroplast operons
Published in The Plant journal : for cell and molecular biology (01-09-2024)“…SUMMARY Achieving optimally balanced gene expression within synthetic operons requires regulatory elements capable of providing a spectrum of expression…”
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Independent translation of ORFs in dicistronic operons, synthetic building blocks for polycistronic chloroplast gene expression
Published in The Plant journal : for cell and molecular biology (01-09-2020)“…SUMMARY We designed a dicistronic plastid marker system that relies on the plastid's ability to translate polycistronic mRNAs. The identification of…”
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Plastid transformation in Nicotiana tabacum and Nicotiana sylvestris by biolistic DNA delivery to leaves
Published in Methods in molecular biology (Clifton, N.J.) (2014)“…The protocol we report here is based on biolistic delivery of the transforming DNA to tobacco leaves, selection of transplastomic clones by spectinomycin…”
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Visual marker and Agrobacterium‐delivered recombinase enable the manipulation of the plastid genome in greenhouse‐grown tobacco plants
Published in The Plant journal : for cell and molecular biology (01-05-2012)“…Summary Successful manipulation of the plastid genome (ptDNA) has been carried out so far only in tissue‐culture cells, a limitation that prevents plastid…”
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A Guide to Choosing Vectors for Transformation of the Plastid Genome of Higher Plants
Published in Plant physiology (Bethesda) (01-12-2007)“…Plastid transformation, originally developed in tobacco (Nicotiana tabacum), has recently been extended to a number of crop species enabling in vivo probing of…”
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Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinase
Published in Methods in molecular biology (Clifton, N.J.) (2021)“…Here we describe a protocol for the excision of plastid marker genes directly in tobacco (Nicotiana tabacum) plants by the Cre recombinase. The example of the…”
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Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering
Published in Methods in molecular biology (Clifton, N.J.) (2021)“…The protocol we report here is based on biolistic delivery of transforming DNA to tobacco leaves, selection of transplastomic clones by spectinomycin or…”
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Plastid marker gene excision in greenhouse-grown tobacco by agrobacterium-delivered Cre recombinase
Published in Methods in molecular biology (Clifton, N.J.) (2014)“…Uniform transformation of the thousands of plastid genome (ptDNA) copies in a cell is driven by selection for plastid markers. When each of the plastid genome…”
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Visual spectinomycin resistance (aadAau) gene for facile identification of transplastomic sectors in tobacco leaves
Published in Plant molecular biology (01-07-2011)“…Identification of a genetically stable Nicotiana tabacum (tobacco) plant with a uniform population of transformed plastid genomes (ptDNA) takes two cycles of…”
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Next generation synthetic vectors for transformation of the plastid genome of higher plants
Published in Plant molecular biology (01-07-2009)“…Plastid transformation vectors are E. coli plasmids carrying a plastid marker gene for selection, adjacent cloning sites and flanking plastid DNA to target…”
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Study of Plastid Genome Stability in Tobacco Reveals That the Loss of Marker Genes Is More Likely by Gene Conversion Than by Recombination between 34-bp loxP Repeats1[C][OA]
Published in Plant physiology (Bethesda) (01-05-2010)“…In transformed tobacco (Nicotiana tabacum) plastids, we flank the marker genes with recombinase target sites to facilitate their posttransformation excision…”
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Study of Plastid Genome Stability in Tobacco Reveals That the Loss of Marker Genes Is More Likely by Gene Conversion Than by Recombination between 34-bp loxP Repeats
Published in Plant physiology (Bethesda) (01-05-2010)“…In transformed tobacco (Nicotiana tabacum) plastids, we flank the marker genes with recombinase target sites to facilitate their posttransformation excision…”
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A Guide to Choosing Vectors for Transformation of the Plastid Genome of Higher Plants1[C][W][OA]
Published in Plant physiology (Bethesda) (01-12-2007)“…Plastid transformation, originally developed in tobacco (Nicotiana tabacum), has recently been extended to a number of crop species enabling in vivo probing of…”
Get full text
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