Search Results - "Tungsuchat‐Huang, Tarinee"

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  1. 1

    Posttranscriptional tuning of gene expression over a large dynamic range in synthetic tobacco chloroplast operons by Yu, Qiguo, TungsuchatHuang, Tarinee, Ioannou, Alexander, Barkan, Alice, Maliga, Pal

    “…SUMMARY Achieving optimally balanced gene expression within synthetic operons requires regulatory elements capable of providing a spectrum of expression…”
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    Journal Article
  2. 2

    Independent translation of ORFs in dicistronic operons, synthetic building blocks for polycistronic chloroplast gene expression by Yu, Qiguo, TungsuchatHuang, Tarinee, Verma, Kanak, Radler, Megan R., Maliga, Pal

    “…SUMMARY We designed a dicistronic plastid marker system that relies on the plastid's ability to translate polycistronic mRNAs. The identification of…”
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    Journal Article
  3. 3

    Plastid transformation in Nicotiana tabacum and Nicotiana sylvestris by biolistic DNA delivery to leaves by Maliga, Pal, Tungsuchat-Huang, Tarinee

    “…The protocol we report here is based on biolistic delivery of the transforming DNA to tobacco leaves, selection of transplastomic clones by spectinomycin…”
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    Journal Article
  4. 4

    Visual marker and Agrobacterium‐delivered recombinase enable the manipulation of the plastid genome in greenhouse‐grown tobacco plants by TungsuchatHuang, Tarinee, Maliga, Pal

    “…Summary Successful manipulation of the plastid genome (ptDNA) has been carried out so far only in tissue‐culture cells, a limitation that prevents plastid…”
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    Journal Article
  5. 5

    A Guide to Choosing Vectors for Transformation of the Plastid Genome of Higher Plants by Lutz, Kerry Ann, Azhagiri, Arun Kumar, Tungsuchat-Huang, Tarinee, Maliga, Pal

    Published in Plant physiology (Bethesda) (01-12-2007)
    “…Plastid transformation, originally developed in tobacco (Nicotiana tabacum), has recently been extended to a number of crop species enabling in vivo probing of…”
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    Journal Article
  6. 6

    Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinase by Tungsuchat-Huang, Tarinee, Maliga, Pal

    “…Here we describe a protocol for the excision of plastid marker genes directly in tobacco (Nicotiana tabacum) plants by the Cre recombinase. The example of the…”
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    Journal Article
  7. 7

    Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering by Maliga, Pal, Tungsuchat-Huang, Tarinee, Lutz, Kerry Ann

    “…The protocol we report here is based on biolistic delivery of transforming DNA to tobacco leaves, selection of transplastomic clones by spectinomycin or…”
    Get more information
    Journal Article
  8. 8

    Plastid marker gene excision in greenhouse-grown tobacco by agrobacterium-delivered Cre recombinase by Tungsuchat-Huang, Tarinee, Maliga, Pal

    “…Uniform transformation of the thousands of plastid genome (ptDNA) copies in a cell is driven by selection for plastid markers. When each of the plastid genome…”
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    Journal Article
  9. 9

    Visual spectinomycin resistance (aadAau) gene for facile identification of transplastomic sectors in tobacco leaves by Tungsuchat-Huang, Tarinee, Slivinski, Kristina Marie, Sinagawa-Garcia, Sugey Ramona, Maliga, Pal

    Published in Plant molecular biology (01-07-2011)
    “…Identification of a genetically stable Nicotiana tabacum (tobacco) plant with a uniform population of transformed plastid genomes (ptDNA) takes two cycles of…”
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    Journal Article
  10. 10

    Next generation synthetic vectors for transformation of the plastid genome of higher plants by Sinagawa-García, Sugey Ramona, Tungsuchat-Huang, Tarinee, Paredes-López, Octavio, Maliga, Pal

    Published in Plant molecular biology (01-07-2009)
    “…Plastid transformation vectors are E. coli plasmids carrying a plastid marker gene for selection, adjacent cloning sites and flanking plastid DNA to target…”
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    Journal Article
  11. 11

    Study of Plastid Genome Stability in Tobacco Reveals That the Loss of Marker Genes Is More Likely by Gene Conversion Than by Recombination between 34-bp loxP Repeats1[C][OA] by Tungsuchat-Huang, Tarinee, Sinagawa-García, Sugey Ramona, Paredes-López, Octavio, Maliga, Pal

    Published in Plant physiology (Bethesda) (01-05-2010)
    “…In transformed tobacco (Nicotiana tabacum) plastids, we flank the marker genes with recombinase target sites to facilitate their posttransformation excision…”
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    Journal Article
  12. 12

    Study of Plastid Genome Stability in Tobacco Reveals That the Loss of Marker Genes Is More Likely by Gene Conversion Than by Recombination between 34-bp loxP Repeats by Tungsuchat-Huang, Tarinee, Sinagawa-García, Sugey Ramona, Paredes-López, Octavio, Maliga, Pal

    Published in Plant physiology (Bethesda) (01-05-2010)
    “…In transformed tobacco (Nicotiana tabacum) plastids, we flank the marker genes with recombinase target sites to facilitate their posttransformation excision…”
    Get full text
    Journal Article
  13. 13

    A Guide to Choosing Vectors for Transformation of the Plastid Genome of Higher Plants1[C][W][OA] by Lutz, Kerry Ann, Azhagiri, Arun Kumar, Tungsuchat-Huang, Tarinee, Maliga, Pal

    Published in Plant physiology (Bethesda) (01-12-2007)
    “…Plastid transformation, originally developed in tobacco (Nicotiana tabacum), has recently been extended to a number of crop species enabling in vivo probing of…”
    Get full text
    Journal Article
  14. 14