Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection

Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection

We propose and demonstrate dendrimer-based coatings for a sensitive biochip surface that enhance the high-performance sorption of small molecules (i.e., biomolecules with low molecular weights) and the sensitivity of a label-free, real-time photonic crystal surface mode (PC SM) biosensor. Biomolecul...

Full description

Saved in:
Bibliographic Details
Published in:Polymers Vol. 15; no. 12; p. 2607
Main Authors: Shakurov, Ruslan, Sizova, Svetlana, Dudik, Stepan, Serkina, Anna, Bazhutov, Mark, Stanaityte, Viktorija, Tulyagin, Petr, Konopsky, Valery, Alieva, Elena, Sekatskii, Sergey, Bespyatykh, Julia, Basmanov, Dmitry
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 08-06-2023
MDPI
Subjects:
Biochips
Biomolecules
biosensor coating
Biosensors
Coatings
Crystal surfaces
dendrimer
Dextran
Dextrans
hyperbranched polymers
Low molecular weights
Microfluidics
Oligonucleotides
PAMAM
PC SM
photonic crystal surface mode biosensor
Photonic crystals
Proteins
Quantum dots
Sensors
Signal to noise ratio
Sorption
Surface plasmon resonance
Russia
Germany
PC SM
label-free detection
dendrimer
oligonucleotides
photonic crystal surface mode biosensor
biomolecular interaction
biosensor coating
microfluidics
PAMAM
hyperbranched polymers
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We propose and demonstrate dendrimer-based coatings for a sensitive biochip surface that enhance the high-performance sorption of small molecules (i.e., biomolecules with low molecular weights) and the sensitivity of a label-free, real-time photonic crystal surface mode (PC SM) biosensor. Biomolecule sorption is detected by measuring changes in the parameters of optical modes on the surface of a photonic crystal (PC). We describe the step-by-step biochip fabrication process. Using oligonucleotides as small molecules and PC SM visualization in a microfluidic mode, we show that the PAMAM (poly-amidoamine)-modified chip's sorption efficiency is almost 14 times higher than that of the planar aminosilane layer and 5 times higher than the 3D epoxy-dextran matrix. The results obtained demonstrate a promising direction for further development of the dendrimer-based PC SM sensor method as an advanced label-free microfluidic tool for detecting biomolecule interactions. Current label-free methods for small biomolecule detection, such as surface plasmon resonance (SPR), have a detection limit down to pM. In this work, we achieved for a PC SM biosensor a Limit of Quantitation of up to 70 fM, which is comparable with the best label-using methods without their inherent disadvantages, such as changes in molecular activity caused by labeling.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2073-4360
2073-4360
DOI:10.3390/polym15122607