Search Results - "Tullman, Jennifer"
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Recovery of an HMWP/hmwBP (pUL48/pUL47) complex from virions of human cytomegalovirus: subunit interactions, oligomer composition, and deubiquitylase activity
Published in Journal of virology (01-08-2014)“…We report that the human cytomegalovirus (HCMV) high-molecular-weight tegument protein (HMWP, pUL48; 253 kDa) and the HMWP-binding protein (hmwBP, pUL47; 110…”
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Strategies for Development of a Next-Generation Protein Sequencing Platform
Published in Trends in biochemical sciences (Amsterdam. Regular ed.) (01-01-2020)“…Proteomic analysis can be a critical bottleneck in cellular characterization. The current paradigm relies primarily on mass spectrometry of peptides and…”
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3
A single amino acid substitution alters ClpS2 binding specificity
Published in Proteins, structure, function, and bioinformatics (01-09-2020)“…ClpS2 is a small protein under development as a probe for selectively recognizing N‐terminal amino acids of N‐degron peptide fragments. To understand the…”
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A ClpS-based N-terminal amino acid binding reagent with improved thermostability and selectivity
Published in Biochemical engineering journal (15-02-2020)“…•Engineering thermostable into ClpS protein variants for biotechnology applications.•Thermostable ClpS variants retain original N-terminal amino acid…”
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Leveraging nature’s biomolecular designs in next-generation protein sequencing reagent development
Published in Applied microbiology and biotechnology (01-09-2020)“…Next-generation approaches for protein sequencing are now emerging that could have the potential to revolutionize the field in proteomics. One such sequencing…”
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Engineering ClpS for selective and enhanced N-terminal amino acid binding
Published in Applied microbiology and biotechnology (01-03-2019)“…One of the central challenges in the development of single-molecule protein sequencing technologies is achieving high-fidelity sequential recognition and…”
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Real-time dynamic single-molecule protein sequencing on an integrated semiconductor device
Published in Science (American Association for the Advancement of Science) (14-10-2022)“…Studies of the proteome would benefit greatly from methods to directly sequence and digitally quantify proteins and detect posttranslational modifications with…”
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Enzymatic protein switches built from paralogous input domains
Published in Biotechnology and bioengineering (01-04-2016)“…ABSTRACT Protein switches have a variety of potential applications in biotechnology and medicine that motivate efforts to accelerate their development…”
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Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA
Published in Biotechnology and bioengineering (01-11-2011)“…We demonstrate that S1 nuclease converts supercoiled plasmid DNA to unit‐length, linear dsDNA through the creation of a single, double‐stranded break in a…”
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Protein switch engineering by domain insertion
Published in Methods in enzymology (2013)“…The switch-like regulation of protein activity by molecular signals is abundant in native proteins. The ability to engineer proteins with novel regulation has…”
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Design rules for protein switch construction and the creation of periplasmic binding protein-beta-lactamase switches
Published 01-01-2011“…Protein engineering techniques can be used to create novel allosterically regulated enzymes by fusing two naturally occurring proteins so that the activity of…”
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Dissertation -
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Tunable Assembly of Peptide-coated Gold Nanoparticles
Published in Plasmonics (Norwell, Mass.) (01-09-2007)Get full text
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A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination
Published in Biotechnology for biofuels (06-06-2016)“…Saccharification of lignocellulosic material by xylanases and other glycoside hydrolases is generally conducted at high concentrations of the final reaction…”
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14
Design rules for protein switch construction and the creation of periplasmic binding protein-beta-lactamase switches
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Dissertation -
15
Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase
Published in Biotechnology for biofuels (15-08-2015)“…Product inhibition can reduce catalytic performance of enzymes used for biofuel production. Different mechanisms can cause this inhibition and, in most cases,…”
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Journal Article