Search Results - "Tullis, Gregory"

Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors by Reed, Sharon E., Staley, Elizabeth M., Mayginnes, John P., Pintel, David J., Tullis, Gregory E.

“…We have developed a simple protocol to transfect mammalian cells using linear polyethylenimine (PEI). Our linear PEI protocol is as effective as commercial…”
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Restoration of SMN Function: Delivery of a Trans-splicing RNA Re-directs SMN2 Pre-mRNA Splicing by Coady, Tristan H, Shababi, Monir, Tullis, Gregory E, Lorson, Christian L

“…Spinal muscular atrophy (SMA) is caused by loss of survival motor neuron-1 (SMN1). A nearly identical copy gene called SMN2 is present in all SMA patients;…”
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Immunological Barriers to Stem Cell Therapy in the Central Nervous System by Tullis, Gregory E., Kirk, Mark D., Spears, Kathleen

“…The central nervous system is vulnerable to many neurodegenerative disorders such as Alzheimer’s disease that result in the extensive loss of neuronal cells…”
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Stimulating Full-Length SMN2 Expression by Delivering Bifunctional RNAs via a Viral Vector by Baughan, Travis, Shababi, Monir, Coady, Tristan H, Dickson, Alexa M, Tullis, Gregory E, Lorson, Christian L

“…Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disorder that is the leading genetic cause of infant mortality. SMA is caused by the loss…”
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Phenotypic characterization of a mouse model of juvenile neuronal ceroid lipofuscinosis by Katz, Martin L, Johnson, Gary S, Tullis, Gregory E, Lei, Bo

“…Abstract Juvenile neuronal ceroid lipofuscinosis (JNCL) is an autosomal recessively inherited neurodegenerative disorder that results from mutations in the…”
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Characterization of the Transcription Profile of Adeno-Associated Virus Type 5 Reveals a Number of Unique Features Compared to Previously Characterized Adeno-Associated Viruses by Qiu, Jianming, Nayak, Ramnath, Tullis, Gregory E, Pintel, David J

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Ocular phenotype in a mouse gene knockout model for infantile neuronal ceroid lipofuscinosis by Lei, Bo, Tullis, Gregory E., Kirk, Mark D., Zhang, Keqing, Katz, Martin L.

“…Mutations in the human protein palmitoyl thioesterase‐1 (PPT‐1) gene result in an autosomal recessive neurodegenerative disorder designated neuronal ceroid…”
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Quantitation of encapsidated recombinant adeno-associated virus DNA in crude cell lysates and tissue culture medium by quantitative, real-time PCR by Mayginnes, John P., Reed, Sharon E., Berg, Heath G., Staley, Elizabeth M., Pintel, David J., Tullis, Gregory E.

“…Recombinant AAV vectors are produced by transient transfection of mammalian cells. The virus is usually purified from a combination of lysed cells and spent…”
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Neural precursors isolated from the developing cat brain show retinal integration following transplantation to the retina of the dystrophic cat by Klassen, Henry, Schwartz, Philip H, Ziaeian, Boback, Nethercott, Hubert, Young, Michael J, Bragadottir, Ragnheidur, Tullis, Gregory E, Warfvinge, Karin, Narfstrom, Kristina

“…The cat has served as an important nonrodent research model for neurophysiology and retinal degenerative disease processes, yet very little is known about…”
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Behavioral assessment in mouse models of neuronal ceroid lipofuscinosis using a light-cued T-maze by Wendt, Kristy D., Lei, Bo, Schachtman, Todd R., Tullis, Gregory E., Ibe, Margaret E., Katz, Martin L.

“…Learning impairment is a common feature of the neuronal ceroid lipofuscinoses (NCL), a family of lysosomal storage disorders associated with progressive…”
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415. Stimulating Full-Length SMN2 Expression by Delivering Bi-Functional RNAs Via a Viral Vector by Baughan, Travis D., Shababi, Monir, Tullis, Gregory E., Lorson, Christian L.

“…Spinal Muscular Atrophy is an autosomal recessive neuromuscular disorder that is the leading genetic cause of infant mortality. SMA is caused by the homozygous…”
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Efficient Replication of Adeno-Associated Virus Type 2 Vectors: a cis-Acting Element outside of the Terminal Repeats and a Minimal Size by Tullis, G E, Shenk, T

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Detection of Calcium Transients in Embryonic Stem Cells and Their Differentiated Progeny by Meyer, Jason S, Tullis, Gregory, Pierret, Christopher, Spears, Kathleen M, Morrison, Jason A, Kirk, Mark D

“…A central issue in stem cell biology is the determination of function and activity of differentiated stem cells, features that define the true phenotype of…”
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Adenovirus E1A proteins interact with the cellular YY1 transcription factor by Lewis, B A, Tullis, G, Seto, E, Horikoshi, N, Weinmann, R, Shenk, T

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Nonsense mutations inhibit splicing of MVM RNA in cis when they interrupt the reading frame of either exon of the final spliced product by NAEGER, L. K, SCHOBORG, R. V, QIHONG ZHAO, TULLIS, G. E, PINTEL, D. J

“…mRNAs R1 and R2 of the autonomous parvovirus minute virus of mice (MVM), which encode the viral nonstructural proteins NS1 and NS2, respectively, are processed…”
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The minor capsid protein VP1 of the autonomous parvovirus minute virus of mice is dispensable for encapsidation of progeny single-stranded DNA but is required for infectivity by TULLIS, G. E, BURGER, L. R, PINTEL, D. J

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The trypsin-sensitive RVER domain in the capsid proteins of minute virus of mice is required for efficient cell binding and viral infection but not for proteolytic processing in vivo by Tullis, G E, Burger, L R, Pintel, D J

“…Analysis of a series of mutations in the trypsin-sensitive RVER region of the amino terminal domain in the capsid proteins (VP1 and VP2) of the autonomous…”
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Post-Transcriptional Regulation of RNA Polymerase II Levels in Caenorhabditis elegans by Dalley, B. K, Rogalski, T. M, Tullis, G. E, Riddle, D. L, Golomb, M

“…To investigate the regulation of RNA polymerase II levels in Caenorhabditis elegans, we have constructed nematode strains having one, two, or three copies of…”
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