The CCR4-NOT complex is implicated in the viability of aneuploid yeasts

To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast Schizosaccharomyces pombe, in which most types of aneuploidy are eventually lethal to the cell. Aneuploids remain viable for a period of time and can form colonie...

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Bibliographic Details
Published in:	PLoS genetics Vol. 8; no. 6; p. e1002776
Main Authors:	Tange, Yoshie, Kurabayashi, Atsushi, Goto, Bunshiro, Hoe, Kwang-Lae, Kim, Dong-Uk, Park, Han-Oh, Hayles, Jacqueline, Chikashige, Yuji, Tsutumi, Chihiro, Hiraoka, Yasushi, Yamao, Fumiaki, Nurse, Paul, Niwa, Osami
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 01-06-2012 Public Library of Science (PLoS)
Subjects:	Aneuploidy Biology Cell Survival - genetics Chromosomes Exoribonucleases - genetics Exoribonucleases - metabolism Gene expression Gene Expression Regulation, Fungal Genetic aspects Genetics Genome, Fungal Health aspects Homologous Recombination - genetics Medicine Meiosis Microbial genetics Mutation Physiological aspects Proteins RNA Stability - genetics RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Saccharomyces Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Schizosaccharomyces - genetics Schizosaccharomyces - growth & development Schizosaccharomyces pombe Proteins - genetics Schizosaccharomyces pombe Proteins - metabolism Sequence Deletion Spores - genetics Spores - growth & development Yeast South Korea Japan
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Summary:	To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast Schizosaccharomyces pombe, in which most types of aneuploidy are eventually lethal to the cell. Aneuploids remain viable for a period of time and can form colonies by reducing the extent of the aneuploidy. We hypothesized that a reduction in colony formation efficiency could be used to screen for gene deletions that compromise aneuploid viability. Deletion mutants were used to measure the effects on the viability of spores derived from triploid meiosis and from a chromosome instability mutant. We found that the CCR4-NOT complex, an evolutionarily conserved general regulator of mRNA turnover, and other related factors, including poly(A)-specific nuclease for mRNA decay, are involved in aneuploid viability. Defective mutations in CCR4-NOT complex components in the distantly related yeast Saccharomyces cerevisiae also affected the viability of spores produced from triploid cells, suggesting that this complex has a conserved role in aneuploids. In addition, our findings suggest that the genes required for homologous recombination repair are important for aneuploid viability.
Bibliography:	Conceived and designed the experiments: YT ON. Performed the experiments: YT AK BG CT ON. Analyzed the data: YT YC YH ON. Contributed reagents/materials/analysis tools: K-LH D-UK H-OP JH YC CT YH FY. Wrote the paper: PN ON.
ISSN:	1553-7404 1553-7390 1553-7404
DOI:	10.1371/journal.pgen.1002776