The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses

The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses

Genome research using appropriately collected pathological tissue samples is expected to yield breakthroughs in the development of biomarkers and identification of therapeutic targets for diseases such as cancers. In this connection, the Japanese Society of Pathology (JSP) has developed “The JSP Gui...

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Published in:Pathology international Vol. 68; no. 2; pp. 63 - 90
Main Authors: Kanai, Yae, Nishihara, Hiroshi, Miyagi, Yohei, Tsuruyama, Tatsuhiro, Taguchi, Kenichi, Katoh, Hiroto, Takeuchi, Tomoyo, Gotoh, Masahiro, Kuramoto, Junko, Arai, Eri, Ojima, Hidenori, Shibuya, Ayako, Yoshida, Teruhiko, Akahane, Toshiaki, Kasajima, Rika, Morita, Kei‐ichi, Inazawa, Johji, Sasaki, Takeshi, Fukayama, Masashi, Oda, Yoshinao
Format: Journal Article
Language:English
Published: Australia Wiley Subscription Services, Inc 01-02-2018
Subjects:
biobank
Biomarkers
Bleeding
Data processing
Deoxyribonucleic acid
DNA
Empirical analysis
Formaldehyde
genome research
Genomes
Guidelines
Handling
HyperText Markup Language
Liquid nitrogen
Operators
Paraffin
pathological tissue sample
Pathology
sample collection and storage
Servers
Target recognition
Technicians
Tissue analysis
empirical analysis
pathological tissue sample
biobank
genome research
sample collection and storage
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Summary:Genome research using appropriately collected pathological tissue samples is expected to yield breakthroughs in the development of biomarkers and identification of therapeutic targets for diseases such as cancers. In this connection, the Japanese Society of Pathology (JSP) has developed “The JSP Guidelines on the Handling of Pathological Tissue Samples for Genomic Research” based on an abundance of data from empirical analyses of tissue samples collected and stored under various conditions. Tissue samples should be collected from appropriate sites within surgically resected specimens, without disturbing the features on which pathological diagnosis is based, while avoiding bleeding or necrotic foci. They should be collected as soon as possible after resection: at the latest within about 3 h of storage at 4°C. Preferably, snap‐frozen samples should be stored in liquid nitrogen (about −180°C) until use. When intending to use genomic DNA extracted from formalin‐fixed paraffin‐embedded tissue, 10% neutral buffered formalin should be used. Insufficient fixation and overfixation must both be avoided. We hope that pathologists, clinicians, clinical laboratory technicians and biobank operators will come to master the handling of pathological tissue samples based on the standard operating procedures in these Guidelines to yield results that will assist in the realization of genomic medicine.
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ISSN:1320-5463
1440-1827
DOI:10.1111/pin.12631