Collaborative environmental DNA sampling from petal surfaces of flowering cherry Cerasus × yedoensis ‘Somei-yoshino’ across the Japanese archipelago

Collaborative environmental DNA sampling from petal surfaces of flowering cherry Cerasus × yedoensis ‘Somei-yoshino’ across the Japanese archipelago

Recent studies have shown that environmental DNA is found almost everywhere. Flower petal surfaces are an attractive tissue to use for investigation of the dispersal of environmental DNA in nature as they are isolated from the external environment until the bud opens and only then can the petal surf...

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Bibliographic Details
Published in:Journal of plant research Vol. 131; no. 4; pp. 709 - 717
Main Authors: Ohta, Tazro, Kawashima, Takeshi, Shinozaki, Natsuko O., Dobashi, Akito, Hiraoka, Satoshi, Hoshino, Tatsuhiko, Kanno, Keiichi, Kataoka, Takafumi, Kawashima, Shuichi, Matsui, Motomu, Nemoto, Wataru, Nishijima, Suguru, Suganuma, Natsuki, Suzuki, Haruo, Taguchi, Y-h., Takenaka, Yoichi, Tanigawa, Yosuke, Tsuneyoshi, Momoka, Yoshitake, Kazutoshi, Sato, Yukuto, Yamashita, Riu, Arakawa, Kazuharu, Iwasaki, Wataru
Format: Journal Article
Language:English
Published: Tokyo Springer Japan 01-07-2018
Springer Nature B.V
Subjects:
Archipelagoes
Biomedical and Life Sciences
Cedar
Chloroplasts - genetics
Collaboration
Cultivars
Cyanobacteria - genetics
Data collection
Deoxyribonucleic acid
Dispersal
DNA
DNA - genetics
DNA sequencing
DNA, Plant - genetics
Ecological monitoring
Ecological studies
Environment
Environmental DNA
Flowering
Flowers & plants
Flowers - genetics
Flowers - microbiology
Genetic testing
Japan
Life Sciences
Plant Biochemistry
Plant Ecology
Plant Physiology
Plant Sciences
Plant species
Pollen
Proteobacteria - genetics
Prunus - genetics
Prunus - microbiology
Sampling
Sequence Alignment
Sequence Analysis, DNA
Technical Note
Crowdsourcing
Environmental DNA
Cherry blossom
Amplicon sequencing
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Description
Summary:Recent studies have shown that environmental DNA is found almost everywhere. Flower petal surfaces are an attractive tissue to use for investigation of the dispersal of environmental DNA in nature as they are isolated from the external environment until the bud opens and only then can the petal surface accumulate environmental DNA. Here, we performed a crowdsourced experiment, the “Ohanami Project”, to obtain environmental DNA samples from petal surfaces of Cerasus  ×  yedoensis ‘Somei-yoshino’ across the Japanese archipelago during spring 2015. C . × yedoensis is the most popular garden cherry species in Japan and clones of this cultivar bloom simultaneously every spring. Data collection spanned almost every prefecture and totaled 577 DNA samples from 149 collaborators. Preliminary amplicon-sequencing analysis showed the rapid attachment of environmental DNA onto the petal surfaces. Notably, we found DNA of other common plant species in samples obtained from a wide distribution; this DNA likely originated from the pollen of the Japanese cedar. Our analysis supports our belief that petal surfaces after blossoming are a promising target to reveal the dynamics of environmental DNA in nature. The success of our experiment also shows that crowdsourced environmental DNA analyses have considerable value in ecological studies.
ISSN:0918-9440
1618-0860
DOI:10.1007/s10265-018-1017-x