The use of electron beam lithographic graft-polymerization on thermoresponsive polymers for regulating the directionality of cell attachment and detachment

The use of electron beam lithographic graft-polymerization on thermoresponsive polymers for regulating the directionality of cell attachment and detachment

Abstract A simple process for nano-patterned cell culture substrates by direct graft-polymerization has been developed using an electron beam (EB) lithography system requiring no photo-masks or EB-sensitive resists. The compound N -isopropylacrylamide (IPAAm) was locally polymerized and grafted dire...

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Bibliographic Details
Published in:Biomaterials Vol. 30; no. 11; pp. 2095 - 2101
Main Authors: Idota, Naokazu, Tsukahara, Takahiko, Sato, Kae, Okano, Teruo, Kitamori, Takehiko
Format: Journal Article
Language:English
Published: Netherlands Elsevier Ltd 01-04-2009
Subjects:
Acrylic Resins - chemistry
Advanced Basic Science
Animals
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Cattle
Cell Adhesion - drug effects
Cell Line
Cell morphology
Cell patterning
Dentistry
Electron beam
Electrons
Mice
Polymerization
Polymers - chemistry
Polymers - pharmacology
Temperature
Thermally responsive material
Tissue Engineering - methods
Electron beam
Polymerization
Thermally responsive material
Cell patterning
Cell morphology
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Summary:Abstract A simple process for nano-patterned cell culture substrates by direct graft-polymerization has been developed using an electron beam (EB) lithography system requiring no photo-masks or EB-sensitive resists. The compound N -isopropylacrylamide (IPAAm) was locally polymerized and grafted directly by EB lithographic exposure onto hydrophilic polyacrylamide (PAAm)-grafted glass surfaces. The size of the surface grafted polymers was controlled by varying the area of EB dose, and a minimal stripe pattern with a 200 nm line-width could be fabricated onto the surface. On the stripe-patterned surfaces, above the lower critical solution temperature (LCST), the cells initially adhered and spread with an orientation along the pattern direction. The magnitude of the spreading angle and elongation of adhered cells depended on the pattern intervals of the grafted PIPAAm. When culture temperature was lower than the LCST, cultured cells detached from the surfaces with strong shrinkage along the pattern direction, and sometimes folded and became parallel with the stripe pattern. This patterned cell recovery technique may be useful for the construction of muscle cell sheets with efficient shrinkage/relaxation in a specific direction and spheroidal 3D cell structures, with application to tissue engineering and microfluidic cellular devices.
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ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2008.12.058