Search Results - "Tsuji, Akihiko"
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Structural basis of EHEP-mediated offense against phlorotannin-induced defense from brown algae to protect akuBGL activity
Published in eLife (01-11-2023)“…The defensive–offensive associations between algae and herbivores determine marine ecology. Brown algae utilize phlorotannin as their chemical defense against…”
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Cellulose-binding activity of a 21-kDa endo-ß-1,4-glucanase lacking cellulose-binding domain and its synergy with other cellulases in the digestive fluid of Aplysia kurodai
Published in PloS one (01-11-2018)“…Endo-ß-1,4-glucanase AkEG21 belonging to glycosyl hydrolase family 45 (GHF45) is the most abundant cellulase in the digestive fluid of sea hare (Aplysia…”
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3
Citrus peel polymethoxyflavones, sudachitin and nobiletin, induce distinct cellular responses in human keratinocyte HaCaT cells
Published in Bioscience, biotechnology, and biochemistry (02-12-2018)“…A variety of polyphenols have been isolated from plants, and their biological activities have been examined. Sudachitin…”
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4
Identification and Characterization of a 25 kDa Protein That Is Indispensable for the Efficient Saccharification of Eisenia bicyclis in the Digestive Fluid of Aplysia kurodai
Published in PloS one (27-01-2017)“…The digestive fluid of the sea hare Aplysia kurodai can liberate approximately 2.5 mg of glucose from 10 mg of dried Eisenia bicyclis powder. Although…”
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Comprehensive enzymatic analysis of the cellulolytic system in digestive fluid of the Sea Hare Aplysia kurodai. Efficient glucose release from sea lettuce by synergistic action of 45 kDa endoglucanase and 210 kDa ß-glucosidase
Published in PloS one (06-06-2013)“…Although many endo-ß-1,4-glucanases have been isolated in invertebrates, their cellulolytic systems are not fully understood. In particular, gastropod feeding…”
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6
Conformational Transition of Giant DNA in a Confined Space Surrounded by a Phospholipid Membrane
Published in Biophysical journal (16-09-2009)“…It has been established that a long DNA molecule exhibits a large discrete conformational change from a coiled state to a highly folded state in aqueous…”
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7
Alisol B, a triterpene from Alismatis rhizoma (dried rhizome of Alisma orientale), inhibits melanin production in murine B16 melanoma cells
Published in Bioscience, biotechnology, and biochemistry (01-03-2017)“…To develop new whitening agents from natural products, we screened 80 compounds derived from crude drugs in Kampo medicine in a melanin synthesis inhibition…”
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Sudachitin, a Polymethoxyflavone from Citrus sudachi, Suppresses Lipopolysaccharide-Induced Inflammatory Responses in Mouse Macrophage-Like RAW264 Cells
Published in Bioscience, biotechnology, and biochemistry (2012)“…Although some polymethoxyflavones possess several important biological properties, including neuroprotective, anticancer, and anti-inflammatory ones,…”
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Estrogen stimuli promote osteoblastic differentiation via the subtilisin-like proprotein convertase PACE4 in MC3T3-E1 cells
Published in Journal of bone and mineral metabolism (01-01-2015)“…Estrogenic compounds include endogenous estrogens such as estradiol as well as soybean isoflavones, such as daidzein and its metabolite equol, which are known…”
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10
Real-Time Monitoring of RNA Synthesis in a Phospholipid-Coated Microdroplet as a Live-Cell Model
Published in Chembiochem : a European journal of chemical biology (15-02-2010)“…We constructed a phospholipid-coated water-in-oil microdroplet (20-60 μm in diameter) that encapsulated plasmid DNA containing a human β-actin cDNA sequence,…”
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11
Purification and characterization of cellulase from North Pacific krill (Euphausia pacifica). Analysis of cleavage specificity of the enzyme
Published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology (01-11-2012)“…Krill are filter feeders that consume algae, plankton and detritus, indicating that krill possess an adequate cellulose digesting system. However, less is…”
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12
Production of n-octanoyl-modified Ghrelin in Cultured Cells Requires Prohormone Processing Protease and Ghrelin O-acyltransferase, as well as n-octanoic Acid
Published in Journal of biochemistry (Tokyo) (01-11-2009)“…Ghrelin was originally isolated from rat stomach as an endogenous ligand for the GH secretagogue receptor. The major active form of ghrelin is a 28-amino acid…”
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13
Enzymatic characterization of germination-specific cysteine protease-1 expressed transiently in cotyledons during the early phase of germination
Published in Journal of biochemistry (Tokyo) (01-01-2013)“…Papain-like cysteine protease activity that shows a unique transient expression profile in cotyledons of daikon radish during germination was detected. The…”
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14
Identification of a plant aminopeptidase with preference for aromatic amino acid residues as a novel member of the prolyl oligopeptidase family of serine proteases
Published in Journal of biochemistry (Tokyo) (01-11-2011)“…Genome analysis has indicated that plants, like animals, possess a variety of protease genes. However, bulk of putative proteases has not been characterized at…”
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15
Structural and biochemical studies on Pompe disease and a "pseudodeficiency of acid α-glucosidase"
Published in Journal of human genetics (01-11-2007)“…We constructed structural models of the catalytic domain and the surrounding region of human wild-type acid alpha-glucosidase and the enzyme with amino acid…”
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Possible role of inter-domain salt bridges in oligopeptidase B from Trypanosoma brucei: critical role of Glu172 of non-catalytic β-propeller domain in catalytic activity and Glu490 of catalytic domain in stability of OPB
Published in Journal of biochemistry (Tokyo) (01-11-2013)“…Oligopeptidase B (OPB) is a member of the prolyl oligopeptidase (POP) family of serine proteases. OPB in trypanosomes is an important virulence factor and…”
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17
Comprehensive enzymatic analysis of the amylolytic system in the digestive fluid of the sea hare, Aplysia kurodai: Unique properties of two α-amylases and two α-glucosidases
Published in FEBS open bio (01-01-2014)“…•The amylolytic system of the digestive fluid of sea hare (Aplysia kurodai) was studied.•Two α-amylases and two α-glucosidases were purified from the digestive…”
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18
Transcriptional regulation of cGMP-dependent protein kinase II (cGK-II) in chondrocytes
Published in Bioscience, biotechnology, and biochemistry (01-01-2010)“…The C-type natriuretic peptide/natriuretic peptide receptor-B/cGMP pathway plays an important role in the regulation of endochondral ossification. In…”
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19
Direct Observation of Specific Messenger RNA in a Single Living Cell under a Fluorescence Microscope
Published in Biophysical journal (01-06-2000)“…We observed the expression of human c- fos mRNA in a living transfected Cos7 cell under a fluorescence microscope by detecting hybrid formed with two…”
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20
Identification of Oligopeptidase B in Higher Plants. Purification and Characterization of Oligopeptidase B from Quiescent Wheat Embryo, Triticum aestivum
Published in Journal of biochemistry (Tokyo) (01-11-2004)“…Proteolytic enzymes in general, and cysteine proteases in particular, play key roles in seed germination and early seedling growth. However, the precise…”
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